A Bone Sample Containing a Bone Graft Substitute Analyzed by Correlating Density Information Obtained by X-ray Micro Tomography with Compositional Information Obtained by Raman Microscopy

Charwat-Pessler, Johann; Musso, Maurizio; Petutschnigg, Alexander; Entacher, Karl; Plank, Bernhard; Wernersson, Lars‐Erik; Tangl, Stefan; Schuller‐Götzburg, Peter

doi:10.3390/ma8073831

Cited by 3 publications

(2 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of animal models seems to be a required step in testing new bone regeneration materials [96]. The in vivo applicability of bioengineered 3D constructs [97] and bone graft substitutes is usually evaluated in animals models as rats, rabbits, mini pigs bone defects, and applications are recorded in dentistry [98][99][100][101][102] as in MFS [103][104][105]. Mainly, the biomaterials and bone grafts investigated for tissue engineering applications are:…”

Section: Biomaterials and Bone Tissue Regenerationmentioning

confidence: 99%

See 1 more Smart Citation

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

Campioni¹,

Pecci

Bedini

2020

Applied Sciences

View full text Add to dashboard Cite

Micro-computed tomography (micro-CT) is a consolidated imaging technology allowing non-destructive three-dimensional (3D) qualitative and quantitative analysis by the observation of microstructures with high resolution. This paper aims at delivering a structured overview of literature about studies performed using micro-CT in dentistry and maxillofacial surgery (MFS) by analyzing the entire set of articles to portray the state of the art of the last ten years of scientific publications on the topic. It draws the scenario focusing on biomaterials, in vitro and in/ex vivo applications, bone structure analysis, and tissue engineering. It confirms the relevance of the micro-CT analysis for traditional research applications and mainly in dentistry with respect to MFS. Possible developments are discussed in relation to the use of the micro-CT combined with other, traditional, and not, techniques and technologies, as the elaboration of 3D models based on micro-CT images and emerging numerical methods. Micro-CT results contribute effectively with whose ones obtained from other techniques in an integrated multimethod approach and for multidisciplinary studies, opening new possibilities and potential opportunities for the next decades of developments.

show abstract

Section: Biomaterials and Bone Tissue Regenerationmentioning

confidence: 99%

“…Raman spectroscopic imaging, a laser-based microscopic device, was applied with micro-CT to examine bone samples and Charwat-Pessler et al [98] reported that the two different imaging techniques provide complementary and mutually supportive information.…”

Section: Micro-ct Analysis Combined With Other Techniques or Technolomentioning

confidence: 99%

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

Campioni¹,

Pecci

Bedini

2020

Applied Sciences

View full text Add to dashboard Cite

show abstract

RESULTS of MORPHOLOGICAL ANALYSIS of ANIMAL HARD TISSUES IN NORMAL AND SIMULATED OSTEOPOROSIS USING A NON-INVASIVE COMPUTED MICROTOMOGRAPHY TECHNIQUE

Долгалев

Rzhepakovsky

Danaev

et al. 2022

IJIM

View full text Add to dashboard Cite

Introduction. X-ray microtomography is a non-destructive method of microstructural analysis, which has a high level of detail and allows the possibility of assessing the internal architecture of organs and tissues using 3D-analysis[1]. The speciﬁcs of working with such equipment can be divided into in vivo and in vitro, i.e. working with live laboratory animals (mice, rats, rabbits) under anesthesia or studying organs and tissues separated from the animal [2].The aim of the work was to study the microstructure of sheep bone tissues in normal and simulated osteoporosis using computed microtomography.Materials and methods. We performed microCT analysis of different sheep bones in normal and experimental osteoporosis. Bone tissue of the jaw, iliac and femur, and teeth were collected from control and experimental animals. Bone tissue samples were ﬁxed in 10% buffered formalin. X-ray microCT scanner Skyscan 1176 (BrukermicroCT, Belgium) and software Skyscan 1176 control program (10.0.0.0), Nrecon (1.7.4.2), DataViewer (1.5.6.2), CT-analyser (1.18.4.0), CTvox (3.3.0r1403) were used to scan and process materials.Results. MicroCT examination and 3D-imaging conﬁrmed the elimination of trabeculae in the metaphyseal region of the femur in sheep with experimental osteoporosis from the centre to the periphery; in addition, 3D-analysis showed a 15.1% decrease in bone percentage, a 7.8% decrease in bone mineral density, and an increase in Tb. Sp. (trabecular separation), Tb. Pf. (trabecular pattern factor) and SMI (structure model index) by 30.2%, 20.8% and 23.6%, respectively, and a decrease in Tb.N. (trabecular number) index by 18.6%, indicating calcium washout, decreased trabecular connectivity and a transition from a lamellar to a rod-like architecture. Similar changes were found in the 3D-analysis of the jaw bone tissue. Thus, a decrease of 18.9% in mineral density was found, as well as a signiﬁcant increase of 11.58 and 2.21 in the indices, particularly Tb. Pf. and SMI. 3D-analysis of iliac microtomography also indicates a simulation of osteoporosis, as evidenced by a signiﬁcant increase in the main indices characterising the development of this pathology.Conclusions. The obtained results not only objectively testify to the development of osteoporosis in the experimental animals, but also indicate signs of the adaptation-compensatory reactions of the body, characterized by appearance of large single trabeculae in the metaphysis of the femur as well as by not expressed reduction of bone mineral density and bone tissue area.

show abstract

High Potential of Bacterial Adhesion on Block Bone Graft Materials

et al. 2020

View full text Add to dashboard Cite

Bone graft infections represent a challenge in daily clinics, resulting in increased patient discomfort and graft removal. The aim of this study was to investigate the initial adhesion of five representative pathogens on three different block bone graft materials (xenogeneic, alloplastic and allogeneic) and to assess if chlorhexidine (CHX) can effectively control the initial bacterial adhesion. Three different block bone grafting materials (Tutobone®, Endobon® and human spongiosa) were incubated with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Pseudomonas aeruginosa in the presence or absence of 0.2% CHX solution. Bacterial adhesion was assessed by the direct counting of the colony-forming units (CFUs) and visualized by scanning electron microscopy (SEM). Overall, the selected bacterial species adhered successfully to all tested bone replacement scaffolds, which showed similar bacterial counts. The lg CFU values ranged from 5.29 ± 0.14 to 5.48 ± 0.72 for E. coli, from 4.37 ± 0.62 to 5.02 ± 0.48 for S. aureus, from 4.92 ± 0.34 to 4.95 ± 0.21 for S. mutans, from 4.97 ± 0.40 to 5.22 ± 0.13 for E. faecalis and from 4.23 ± 0.54 to 4.58 ± 0.26 for P. aeruginosa. CHX did not interfere with initial microbial adhesion, and yet it killed all adhered bacterial cells. Thus, CHX can be used to prevent subsequent biofilm infections.

show abstract

A Bone Sample Containing a Bone Graft Substitute Analyzed by Correlating Density Information Obtained by X-ray Micro Tomography with Compositional Information Obtained by Raman Microscopy

Cited by 3 publications

References 51 publications

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

RESULTS of MORPHOLOGICAL ANALYSIS of ANIMAL HARD TISSUES IN NORMAL AND SIMULATED OSTEOPOROSIS USING A NON-INVASIVE COMPUTED MICROTOMOGRAPHY TECHNIQUE

High Potential of Bacterial Adhesion on Block Bone Graft Materials

Contact Info

Product

Resources

About