Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Kimura-Suda, Hiromi; Kajiwara, M.; Sakamoto, Naoya; Kobayashi, Seigo; Ijiro, Kuniharu; Yurimoto, Hisayoshi; Yamato, Hideyuki

doi:10.1016/j.job.2013.04.006

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…Figure 1 shows FTIR images of the chemical composition of the sham rat femur (a) and CKD rat femur (b) showing the distribution of phosphate (PO 4 3− ). The image is comprised of color pixels corresponding to the intensity of a selected PO 4 3− band in the FTIR spectrum. The FTIR images of the femurs demonstrate that the total phosphate in the trabecular bone and cortical bone was lower in CKD rats than in the sham rats.…”

Section: Resultsmentioning

confidence: 99%

“…The mineral-to-collagen matrix ratio (PO 4 3− /amide I) was calculated by integrating the area of the phosphate band (PO 4 3− , 1180-911 cm −1 ) and dividing it by the area of the amide I band (1709-1606 cm −1 ). The carbonateto-phosphate ratio (CO 3 2− /PO 4 3− ) was calculated by integrating the area of the carbonate band (CO was obtained from the amide I bands (1709-1606 cm −1 ), which were collected by two polarized IR beams (0 • and 90…”

Section: Methodsmentioning

confidence: 99%

“…Bone strength, which is a barometer of bone health, primarily reflects the integration of two features: bone quantity (bone mineral density) and bone quality [2,3]. FTIR imaging is a powerful tool for the characterization of bone quality, including parameters such as the mineral-to-collagen matrix ratio, carbonateto-phosphate ratio in the hydroxyapatite, crystallinity, and collagen maturity [1,[4][5][6][7]. In our previous work [1], we used FTIR imaging to characterize the bone quality of the femur in a rat model of CKD induced by 5/6 nephrectomy (Nx).…”

Section: Introductionmentioning

confidence: 99%

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Collagen Fiber Orientation in the Femur of Rats with Chronic Kidney Disease

Kimura-Suda

Ito

Kanazawa

et al. 2015

e-J. Surf. Sci. Nanotech.

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Collagen fiber orientation in the femur of chronic kidney disease (CKD) rats was determined using Fourier transform infrared (FTIR) imaging with a wire grid polarizer in order to characterize bone quality in CKD. Elevenweek-old male rats underwent 5/6 nephrectomy (Nx) to replicate CKD. These CDK rats, as well as sham-operated rats (sham), were kept for 16 weeks. After sacrifice, femurs in the sham and CKD rats were removed and embedded in PMMA. Longitudinal sections (∼3 µm) of the femurs were prepared for FTIR imaging. IR dichroism images were calculated from the FTIR images obtained using two polarized IR beams (0 • and 90 • ). The IR dichroism images of the collagen fibers in the CKD rat femurs indicated that the collagen fibers in the cortical bone were aligned longitudinally, and the degree of collagen fiber orientation was decreased in the metaphysis compared to that in the diaphysis. The carbonate-to-phosphate ratio in the hydroxyapatite was reduced in both the cortical bone and trabecular bone of the CKD rat femurs. In the cortical bone, reductions in the carbonate-to-phosphate ratio and collagen fiber orientation were particularly evident in the metaphysis in the CKD rats.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Collagen Fiber Orientation in the Femur of Rats with Chronic Kidney Disease

Kimura-Suda

Ito

Kanazawa

et al. 2015

e-J. Surf. Sci. Nanotech.

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“…Fourier transform infrared (FTIR) spectroscopy has been extensively used in the analysis of molecular components of biomaterials and tissues (8). Studies on bone metabolism and chemical and structural changes caused by bone aging and changes caused by diseases, such as osteoporosis and effects of therapies, were performed in mineralized tissues with FTIR (9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Strontium Ranelate Effect on the Repair of Bone Defects and Molecular Components of the Cortical Bone of Rats

et al. 2016

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This study was conducted to evaluate the effects of treatment with strontium ranelate (SR) on the repair of bone defects and molecular components of bones in femurs. Adult female rats (n=27) were subjected to ovariectomy (OVX) or Sham surgery. Thirty days after surgery, a defect was made in the femur and the animals were then divided into three groups: OVX, SHAM and OVX+SR. Euthanasia was performed four weeks after the bone defect surgery. Repair in bone defect was assessed by computed microtomography (μCT) and chemical composition of cortical bone was analyzed by Fourier transform infrared (FTIR) spectroscopy and energy dispersive X-ray spectroscopy (EDS). The trabecular thickness (Tb.Th) of the newly formed bone in the OVX+SR group was significantly higher than that for the OVX group. The collagen maturity in the OVX+SR group was smaller than in the other two groups. In this group, a significant increase in the amount of strontium (Sr) and a decrease in the amount of calcium (Ca) embedded to bone tissue were also observed. Systemic treatment with SR improved microarchitecture of the newly formed bone inside the defect, but decreased cross-linking of mature collagen in cortical bone.

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“…Em tecidos mineralizados foram realizados estudos do metabolismo ósseo e das alterações químicas e estruturais causadas com o envelhecimento do osso e também das modificações causadas por patologias como a osteoporose e os efeitos das terapias (Camacho et al, 2003;Mendelsohn, 2005;Paschalis et al, 2011;KimuraSuda et al, 2013 (Veloso, 2013;Kimura-Suda et al, 2013). A figura 2.1 mostra um espectro representativo de FTIR do osso com as principais frequências de interesse.…”

Section: Espectroscopia No Infravermelhounclassified

Influência do ranelato de estrôncio na reparação de defeitos ósseos em fêmures e nos componentes moleculares do osso em ratas ovariectomizadas

Rosa¹

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Rosa JA. Influence of strontium ranelate on the repair of bone defects in femurs and molecular components of bone in ovariectomized rats [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2014. Versão Corrigida.Strontium ranelate (SR) decreases bone resorption and at the same time acts as an anabolic agent in this tissue. This study was conducted to evaluate the effects of ovariectomy and treatment with SR on the repair of bone defects and molecular components of bones in femurs. Adult female rats (n=27) were subjected to ovariectomy or Sham surgery. Thirty days after surgery, a defect was made in the femur and the animals were divided into three groups: ovariectomy (OVZ), Sham surgery (SHAM) and ovariectomy plus treatment with SR, 625 mg / kg / day (SR).Euthanasia was performed four weeks after surgery to cause the bone defect. Repair in bone defect was assessed by computed microtomography (μCT) and the chemical composition of bone tissue was analyzed by Fourier transform infrared (FTIR) spectroscopy and energy dispersive X-ray spectroscopy (EDS). The SHAM group showed mean values for bone volume (BV) higher than those of the OVZ group (p = 0.014) and the relative bone volume (BV / TV) was about 8% higher than that of the OVZ group. Comparison between SHAM and SR showed borderline values for these variables. In the SR group, the average value for trabecular thickness (Tb.Th) was significantly higher (~1.8%) than that in the OVZ (p = 0.049) group and did not differ from that of the SHAM group. Significant difference was not observed in the relation between inorganic and organic compounds in the three groups. The ratio of the areas of the bands in 1057 and 1023 cm-1 for the calculation of IC was 1,024 in the SHAM group, 1,015 in the RS group and 1,108 in OVZ group. In the study of the amide III band, a change in the proportion of secondary structure of matrix proteins was observed in ovariectomized animals, possibly a decrease in of α-helical and random coil (RC) and an increase in β-sheet structures. The collagen cross-linking network maturity estimated by the relation between amide I subbands (1660/1690) showed the smaller number in the SR group. In this group, a significant increase in the amount of strontium (Sr) and a decrease in the amount of calcium (Ca) embedded to bone tissue, as assessed by the EDS technique, were also observed. The results demonstrate that systemic treatment with SR promoted bone repair and improved microarchitecture of the newly formed bone within the defect. However, results of analyses with FTIR suggest that the SR has not reversed the alterations caused by ovariectomy in the organic matrix and decreased cross-linking of collagen mature.

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Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Cited by 10 publications

References 27 publications

Collagen Fiber Orientation in the Femur of Rats with Chronic Kidney Disease

Collagen Fiber Orientation in the Femur of Rats with Chronic Kidney Disease

Strontium Ranelate Effect on the Repair of Bone Defects and Molecular Components of the Cortical Bone of Rats

Influência do ranelato de estrôncio na reparação de defeitos ósseos em fêmures e nos componentes moleculares do osso em ratas ovariectomizadas

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