Viability of Carica papaya L. Seeds by the Tetrazolium Test

Abstract

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome

“…Several studies have demonstrated that tetrazolium test is well suited to determine seed viability. For example, the viability of Carica papaya [ 25 ], Nitraria tangutorum , and Nitraria sibirica seed lots [ 27 ] were assessed using the tetrazolium test. Consistent with our findings, these authors demonstrated that a 1% tetrazolium solution worked efficiently and quickly in evaluating the viability and quality of these seed lots prior to germination evaluations.…”

“…Viable seeds were stained bright red, while non-viable or dead seeds were unstained or stained white or light red. These evaluations led to three classification of seeds as follows: totally stained, unstained, and partially stained [ 25 ].…”

“…Twenty-five seeds (25) from each species were primed overnight in glass vials containing 8 mL solutions of either 20 µg/mL or 40 µg/mL of CNTs dissolved in DI water [5]. CNTs were thoroughly dissolved in DI water following sonication for 15 min (Qsonica Q700, Model: CL-334, Boston Laboratory Equipment, Newtown, CT, USA).…”

Carbon Nanoparticles Functionalized with Carboxylic Acid Improved the Germination and Seedling Vigor in Upland Boreal Forest Species

“…Viable seed stained bright red, while pink and very dark red stains are indicative of dead tissue. As such, seeds were classified into three categories based on the following staining patterns: completely stained, completely unstained, and partially stained [55].…”