Nanopriming has been shown to significantly improve seed germination and seedling vigor in several agricultural crops. However, this approach has not been applied to native upland boreal forest species with complex seed dormancy to improve propagation. Herein, we demonstrate the effectiveness of carbon nanoparticles functionalized with carboxylic acids in resolving seed dormancy and improved the propagation of two native upland boreal forest species. Seed priming with carbon nanoparticles functionalized with carboxylic acids followed by stratification were observed to be the most effective in improving germination to 90% in green alder (Alnus viridis L.) compared to 60% in the control. Conversely, a combination of carbon nanoparticles (CNPs), especially multiwall carbon nanoparticles functionalized with carboxylic acid (MWCNT–COOH), cold stratification, mechanical scarification and hormonal priming (gibberellic acid) was effective for buffaloberry seeds (Shepherdia canadensis L.). Both concentrations (20 µg and 40 µg) of MWCNT–COOH had a higher percent germination (90%) compared to all other treatments. Furthermore, we observed the improvement in germination, seedling vigor and resolution of both embryo and seed coat dormancy in upland boreal forest species appears to be associated with the remodeling of C18:3 enriched fatty acids in the following seed membrane lipid molecular species: PC18:1/18:3, PG16:1/18:3, PE18:3/18:2, and digalactosyldiacylglycerol (DGDG18:3/18:3). These findings suggest that nanopriming may be a useful approach to resolve seed dormancy issues and improve the seed germination in non-resource upland boreal forest species ideally suited for forest reclamation following resource mining.
Application of the nanopriming technique to alleviate seed dormancy has shown promising results in various agricultural crop species. However, there is a dearth of knowledge regarding its potential use in native peatland boreal forest species to alleviate seed dormancy and improve their propagation or vigor for forest reclamation activities. Herein, we demonstrate the use of nanopriming with carbon nanotubes (CNT) to alleviate seed dormancy, improved seed germination, and seedling vigor in two boreal peatland species. Bog birch (Betula pumila L.) and Labrador tea (Rhododendron groenlandicum L.) seeds with embryo or seed coat dormancy were nanoprimed with either 20 or 40 µg/mL CNT, cold stratified at 2–4 °C for 15 days, and allowed to germinate at room temperature. The emerged seedlings’ lipidome was assessed to decipher the role of lipid metabolism in alleviating seed dormancy. We observed significant (p < 0.05) improvement in seedling germination and seedling vigor in seeds primed with multiwalled carbon nanotubes functionalized with carboxylic acids. Phosphatidylcholine (PC 18:1/18:3), phosphatidylglycerol (PG 16:1/18:3), and lysophosphatidylcholine (LPC 18:3) molecular species (C18:3 enriched) were observed to be highly correlated with the increased seed germination percentages and the enhanced seedling vigor. Mechanistically, it appears that carbon nanoprimed seeds following stratification are effective in mediating seed dormancy by remodeling the seed membrane lipids (C18:3 enriched PC, PG, and LPC) in both peatland boreal forest species. The study results demonstrate that nanopriming may provide a solution to resolve seed dormancy issues by enhancing seed germination, propagation, and seedling vigor in non-resource boreal forest species ideally suited for forest reclamation following anthropogenic disturbances.
A pot experiment was conducted at Plant Breeding and Biotechnology Laboratory of Agrotechnology Discipline, Khulna University, during the period from Yellow Anne) were used as treatment variables. Layout system was Completely Randomized Design (CRD) under factorial arrangement with three replications. Spray formulation had significant influence on growth of the two cultivars tested. Significantly highest plant height was obtained (39.83 cm) at 290 DAP from Mokara Diehard Red Variety with spray formulation 3 compared to other spray formulations. Highest leaf area index (0.16) was obtained from Mokara Diehard Red Variety with spray formulation 3 at 290 DAP mainly due to increased number of leaf and total leaf area. Mokara Diehard Red cultivar with spray formulation 2 enhanced plant growths and resulted greater leaf number (21.33) and leaf length (26.83 cm) to other cultivars cultivated. Plant height as well as other growth parameters was gradually increased due to suitable treatment combination. Results of the study revealed that spray formulation 3 would be suitable for Mokara Diehard Red cultivars cultivation under pot condition.
Internet of things (IoT) is not a single word, but it has gathered billions of devices in the same lane. The Internet of things has given the lives of things. Machines have a sense now like a human. It works remotely as the program has been settled inside the chip. The system has become so smart and reliable. The Internet of things has brought out changes in most of the sectors of humankind. Meanwhile, agriculture is the main strength of a country. The more the production of agricultural products increased, the world will be more completeness from food shortage. The production of agriculture can be increased when the IoT system can be entirely implemented in the agricultural sector. Most of the approaches for IoT based agriculture have been reviewed in this paper. Related to IoT based agriculture, most of the architecture and methodology have been interpreted and have been critically analyzed based on previous related work of the researchers. This paper will be able to provide a complete idea with the architecture and methodology in the field of IoT based agriculture. Moreover, the challenges for agricultural IoT are discussed with the methods provided by the researchers.
Wireless Fidelity (Wi-Fi) fingerprinting is a remarkable approach developed by modern science to detect the user’s location efficiently. Today, the Global Positioning System (GPS) is used to keep track of our current location for outdoor positioning. In GPS technology, satellite signals cannot reach indoor environments as they are shielded from obstructions so that indoor environments with a lack of Line of Sight (LoS) do not provide enough satellite signal accuracy. Since indoor environments are very difficult to track, thus, a wide variety of techniques for dealing with them have been suggested. The best way to offer an indoor positioning service with the current technology is Wi-Fi since most commercial infrastructure is well equipped with Wi-Fi routers. The use of Wi-Fi fingerprinting techniques is becoming more common in the field of indoor positioning systems (IPS). For the purpose of indoor location localization, each method for obtaining a Wi-Fi fingerprint has been analyzed and discussed in this work. In this study, the majority of the algorithms that are associated with Wi-Fi fingerprinting have been interpreted, and the earlier works of other researchershave been critically evaluated, in order to get a more in-depth understanding of how the Wi-Fi fingerprinting process works.
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