Versatile strategies for bioproduction of hyaluronic acid driven by synthetic biology

Yao, Zhi Yuan; Qin, Jiufu; Gong, Jin Song; Ye, Yun Hui; Qian, Jian Ying; Li, Heng; Xu, Zheng; Shi, Jin Song

doi:10.1016/j.carbpol.2021.118015

Cited by 37 publications

(21 citation statements)

References 129 publications

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“…However, the key constraint of producing HA of relatively uniform length has not yet been resolved. In addition, obstacles regarding the viscosity of the medium over 4 g L −1 of HA that limits the oxygen transfer creates an anaerobic environment that directs the carbon flow more to the production of biomass and not to HA, with the corresponding creation of contaminating by-products such as lactate [ 24 , 26 ]. The production of HA through different microbial routes and fermentations was summarized in ( Table 1 ).…”

Section: Microorganisms and Biosynthesis Of Hyaluronic Acidmentioning

confidence: 99%

“…After that, it was obtained by fermentation using pathogenic bacteria. However, both methods of HA production require extensive purification protocols to prevent toxin contamination which are expensive and may cause cross infections [ 24 ]. Recently, the possibility of producing HA from microorganisms appeared, which offered the opportunity to create a more straightforward, economical, without raw material limitations, and environmentally favorable [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, both methods of HA production require extensive purification protocols to prevent toxin contamination which are expensive and may cause cross infections [ 24 ]. Recently, the possibility of producing HA from microorganisms appeared, which offered the opportunity to create a more straightforward, economical, without raw material limitations, and environmentally favorable [ 24 ]. HAs disaccharide units show the structural property in ß configuration, a very energetically stable structure with functional groups of (carboxyl, hydroxyl, acetamido, and anomeric carbon).…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive review on biotechnological production of hyaluronic acid: status, innovation, market and applications

et al. 2022

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The growing, existing demand for low-cost and high-quality hyaluronic acid (HA) needs an outlook of different possible production strategies from renewable resources with the reduced possibility of cross-infections. Recently, the possibility of producing HA from harmless microorganisms appeared, which offers the opportunity to make HA more economical, without raw material limitations, and environmentally friendly. HA production is mainly reported with Lancefield Streptococci A and C , particularly from S. equi and S. zooepidemicus . Various modes of fermentation such as batch, repeated batch, fed-batch, and continuous culture have been investigated to optimize HA production, particularly from S. zooepidemicus , obtaining a HA yield of 2.5 g L −1 – 7.0 g L −1 . Among the different utilized DSP approaches of HA production, recovery with cold ethanol (4°C) and cetylpyridinium chloride is the ideal strategy for lab-scale HA production. On the industrial scale, besides using isopropanol, filtration (0.22 um), ultrafiltration (100 kDa), and activated carbon absorption are employed to obtain HA of low molecular weight and additional ultrafiltration to purify HA of higher MW. Even though mature technologies have already been developed for the industrial production of HA, the projections of increased sales volume and the expansion of application possibilities require new processes to obtain HA with higher productivity, purity, and specific molecular weights. In this review, we have put forth the progress of HA technological research by discussing the microbial biosynthetic aspects, fermentation and downstream strategies, industrial-scale scenarios of HA, and the prospects of HA production to meet the current and ongoing market demands.

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Section: Microorganisms and Biosynthesis Of Hyaluronic Acidmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comprehensive review on biotechnological production of hyaluronic acid: status, innovation, market and applications

et al. 2022

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“…Other polysaccharide-based materials are alginate, chitin, chitosan, or hyaluronan. The production and properties of these materials can be engineered as well using synthetic biology strategies as recently reviewed elsewhere [ [59] , [60] , [61] ].…”

Section: Engineering Cells To Synthesize (Precursors Of) Non-living Materialsmentioning

confidence: 99%

Synthetic biology as driver for the biologization of materials sciences

Burgos-Morales

Gueye

Lacombe

et al. 2021

Materials Today Bio

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Materials in nature have fascinating properties that serve as a continuous source of inspiration for materials scientists. Accordingly, bio-mimetic and bio-inspired approaches have yielded remarkable structural and functional materials for a plethora of applications. Despite these advances, many properties of natural materials remain challenging or yet impossible to incorporate into synthetic materials. Natural materials are produced by living cells, which sense and process environmental cues and conditions by means of signaling and genetic programs, thereby controlling the biosynthesis, remodeling, functionalization, or degradation of the natural material. In this context, synthetic biology offers unique opportunities in materials sciences by providing direct access to the rational engineering of how a cell senses and processes environmental information and translates them into the properties and functions of materials. Here, we identify and review two main directions by which synthetic biology can be harnessed to provide new impulses for the biologization of the materials sciences: first, the engineering of cells to produce precursors for the subsequent synthesis of materials. This includes materials that are otherwise produced from petrochemical resources, but also materials where the bio-produced substances contribute unique properties and functions not existing in traditional materials. Second, engineered living materials that are formed or assembled by cells or in which cells contribute specific functions while remaining an integral part of the living composite material. We finally provide a perspective of future scientific directions of this promising area of research and discuss science policy that would be required to support research and development in this field.

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“…Because mass production of naturally-derived biomaterials could be achieved through microbial fermentation technologies with high yield, low cost and less-limited raw material sources, the manufacture of some naturally-derived biomaterials including collagens are inclined to shift from animal tissue extraction to biosynthesis. For example, sodium hyaluronate was firstly found and extracted from rooster combs, but the majority of the commercial medical-grade sodium hyaluronate is now manufactured with synthetic biology [ 13 , 14 ]. With the advancement of technologies, it is expected that rCols may play an increasingly important role in the medical and healthcare field [ 15 ].…”

mentioning

confidence: 99%