Vehicle influence on calcium hydroxide pastes diffusion in human and bovine teeth

Abstract

The purpose of this study was to compare the pH and calcium ion liberation after use of calcium hydroxide pastes with different paste vehicles in human or bovine teeth. Ninety-two single-rooted human and bovine roots were used. The roots were instrumented and an external cavity preparation was performed. The roots were divided in to human and bovine groups. Each group was subdivided into four subgroups (SB) according to the vehicle:SB1, detergent; SB2, saline; SB3, polyethylenoglycol + camphorated paramonochlorophenol (Calen PMCC) and SB4, polyethylenoglycol + furacyn paramonochlorophenol (FPMC). Specimens were immersed into saline solution at 37 degrees C and after 7 and 14 days pH and calcium ion measurements were made. The results were analyzed by anova and Tukey tests (P < 0.05). There was no statistical difference between bovine and human teeth in the pH analysis (P < 0.05), but bovine teeth provided larger calcium ion liberation than human teeth. Calen PMCC was statistically more effective for pH increase and calcium ion liberation in all analyses, followed by FPMC and saline. Detergent showed the lowest pH alterations and calcium ion liberation. The period of 14 days showed more calcium ionic liberation than the 7-day period.

Bovine teeth as substitute for human teeth in dental research: a review of literature

“…Therefore, 68 papers were included in the final review. The studies covered seven main categories: morphology (5 studies) (22)(23)(24)(25)(26), chemical composition (11 studies) (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37), physical properties (12 studies) (38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49), dental caries (7 studies) (6,9,(50)(51)(52)(53)(54), dental erosion/abrasion (10 studies) (55)(56)(57)(58)(59)(60)(61)(62)(63)(64), bonding/adhesive strength (17 studies) (65)(66)(67)(68)…”

“…Eleven studies were reviewed (27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37). Bisaz et al (27) showed detectable amounts of inorganic pyrophosphate in enamel and dentin of both human and bovine teeth.…”

“…However, the study compared primary human teeth to bovine teeth and did not clarify if primary or permanent bovine teeth were used. Camargo et al (33) compared the calcium ion release and the pH of storage solutions between human and bovine teeth after the application of calcium hydroxide pastes with four different paste vehicles. The study revealed no significant difference between bovine and human teeth in the pH measurement, but bovine teeth showed significantly higher calcium ion release compared to human teeth.…”

Descontaminação intra-tubular de dentes bovinos por pastas de hidróxido de calcio associadas a diferentes veículos

“…Outro estudo (CAMARGO et al, 2006) não encontrou diferenças entre os dentes humanos e os bovinos em relação a difusão de íons através da dentina. Além disso, a estrutura dentinária principal de dentes bovinos e humanos não são significativamente diferentes (HAAPASALO e ORSTAVIK, 1987).…”

Tempo de ação antimicrobiana de pastas de hidróxido de cálcio associadas a agitação ultrassônica

“…A fim de obter-se uma maior descontaminação dos espécimes utilizados, para que não houvesse interferências de outros micro-organismos previamente existentes, após a instrumentação dos tubos de dentina e previamente a esterilização, foram submetidos a um banho ultrassônico por 10 minutos imersos em (HAAPASALO e ØRSTAVIK, 1987), pois a dentina bovina é reconhecida pela similaridade com a dentina humana na estrutura e composição (EVANS et al, 2003), e não mostram diferenças na difusão de pH, apesar do diâmetro maior dos túbulos bovinos (CAMARGO et al, 2006). Os dentes foram seccionados duas vezes, a primeira para remoção dos 5mm apicais e a segunda para dar origem aos espécimes de tubos de dentina de 12mm de comprimento.…”