VEGF as a potential target in lung cancer

Frezzetti, Daniela; Gallo, Marianna; Maiello, Monica R.; D’Alessio, Amelia; Esposito, Claudia; Chicchinelli, Nicoletta; Normanno, Nicola; Luca, Antonella De

doi:10.1080/14728222.2017.1371137

Cited by 176 publications

(109 citation statements)

References 77 publications

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“…VEGF, PDGF and IGF gene expressions were found higher in uses of low dose dextrose than in control (Table-1). These genes are active in angiogenesis and healing process (29,30). Due to our analyses, dextrose increases angiogenesis and apopitosis via VEGF, PDGF, IGF, CASP3 and CASP8 genes.…”

Section: Discussionmentioning

confidence: 68%

Dextrose solution used for prolotherapy decreases cell viability and increases gene expressions of angiogenic and apopitotic factors

Guran¹,

Çoban²,

Karasimav³

et al. 2018

Gulhane Med J

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Dextrose solutions (in 1%, 5%, 10%, 15%, 20% and 25% concentrations) were applied on adult human fibroblasts in vitro culture condition. Cell proliferation assay was used for finding the cell deaths in cell culture. Up to 80% fibroblast cells died in high dextrose concentrations (15%, 20% and 25%). The deaths of cells were visualized on inverted microscope. In low dextrose concentrations (1%, 5% and 10%), viable cell ratio was above 80%. The gene expression analysis were performed on selected genes which have roles on angiogenesis and apopitosis [VEGFA (Vascular Endothelial Growth Factor A-OMIM ABSTRACTObjectives: Hypertonic dextrose injection in prolotherapy is an injection-based treatment used in chronic musculoskeletal conditions. Dextrose prolotherapy raises growth factor levels and enhances tissue repair, reduces musculoskeletal pain. Despite of uses for many years, the effect of dextrose solution on cellular and molecular base is not fully clear. Here, the roles of dextrose solutions was tried to find out in different concentrations on human fibroblasts in vitro. Gene expression alterations were analyzed in uses of dextrose solutions on growth and apoptotic factors. Methods:The effects of dextrose solution (1%, 5%, 10%-low doses, 15%, 20% and 25%-high doses) were evaluated in vitro by using human fibroblast culture. In each condition total RNA extraction and cDNA synthesis were performed. The gene expression levels of angiogenic and apoptotic factors were analyzed by using real-time polymerase chain reaction. The gene expression results of growth and apoptotic factors were correlated with control results. Results:Results;Dextrose solutions were affected the viability of fibroblast cells in culture flask in high concentrations. In high doses dextrose concentrations, up to 80% of fibroblasts were died because of toxic conditions. Viable fibroblast cell ratios were decreased proportionally due to the dextrose concentration. Low dextrose concentrations increased gene expressions in angiogenic (VEGFA, PDGFA, PDGFB, IGF1) and in apopitotic factors (CASP3 and CASP8) in fibroblasts. Conclusions: Conclusion; Dextrose solution in high concentrations, decreases viable cell ratios on adult fibroblast cell line. Dextrose solutions in proper concentrations increase the gene expressions of angiagenic and apopitotic factors on viable cells in adult fibroblast cell culture.

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Section: Discussionmentioning

confidence: 68%

Dextrose solution used for prolotherapy decreases cell viability and increases gene expressions of angiogenic and apopitotic factors

Guran¹,

Çoban²,

Karasimav³

et al. 2018

Gulhane Med J

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“…Angiogenesis is mainly regulated by several different growth factors. e VEGF family and its receptors are likely to be the most important tissue factors responsible for angioblast differentiation and lymphangiogenesis, which activates the tyrosine kinase receptors of endothelial cells and induces endothelial cell growth and invasion [24,25]. PDGF and its receptor play an important regulatory role in tumour angiogenesis, stimulating vascular endothelial cell growth and chemotaxis and contributing to tumour angiogenesis and remodelling [26,27].…”

Section: Discussionmentioning

confidence: 99%

Antiangiogenesis Roles of Exosomes with Fei-Liu-Ping Ointment Treatment are Involved in the Lung Carcinoma with the Lewis Xenograft Mouse Model

Zheng

Liu²,

Fan

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Exosomes display efficient biocompatibility and represent valuable vehicles for drug or effective material delivery in a tumourtherapeutic approach. Following treatment with Fei-Liu-Ping (FLP) ointment, a traditional Chinese herbal formula, which is used for treating lung cancer patients, could inhibit lung carcinoma growth in clinical and animal studies. In the present study, the values of VEGF and PDGF, which were closely related to angiogenesis, were estimated in serum and carcinoma tissue exosomes to unveil the FLP effects on angiogenesis. e common inflammatory factors of IL-6, IL-1β, TNF-α, and TGF-β in serum exosomes were also detected with the Lewis xenograft model. Methods. Male C57BL/6 mice were randomly divided into four groups, namely, normal, model, cyclophosphamide (CTX), and FLP treatment groups. Histological structures were observed and imaged by H&E. CD31 expressions in tumour tissues were detected by immunofluorescence (IF) and western blot (WB). VEGF, PDGF, and PDGFR levels in exosomes, serum, tumour, and lung tissues were detected by enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and WB, respectively. IL-6, IL-1β, TNF-α, and TGF-β levels in exosomes were measured by multiplex immunoassay panels. Results. e results showed that FLP had tumour growth inhibition rate (39.31%). CD31 protein expression was obviously decreased in tumour tissues of CTX-and FLP-treated MO mice, compared to that of MO mice (P < 0.05 or P < 0.001). VEGF, PDGF, and PDGFR expression levels with FLP treatment were downregulated in exosomes, serum, tumour, and lung tissues compared to model group (P < 0.05 or P < 0.01). e expressions of IL-6, IL-1β, and TNF-α were downregulated in exosomes compared to the model group (P < 0.05 or P < 0.01). Conclusions. is study suggested that FLP had the ability of inhibiting tumourigenesis in a Lewis lung xenograft mouse model, whose therapeutic mechanisms might relate with the downregulation of angiogenesis factor and tumour inflammatory cytokines levels.

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“…CCKR signaling map, insulin/IGF pathway-protein kinase B signaling cascade, apoptosis signaling pathway, p53 pathway feedback loops 2, oxidative stress response, and VEGF signaling pathway were related to mentioned targets which are important pathways in physiology and pathophysiology of lung. 48,[65][66][67][68][69][70] Therefore, regulators of the targets play important roles in lung cancer.…”

Section: Discussionmentioning

confidence: 99%

A panel of noncoding RNAs in non–small‐cell lung cancer

Bagheri

Khorshid

Tavallaie

et al. 2018

J of Cellular Biochemistry

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Non–small‐lung cancer (NSCLC) is the leading cause of cancer death. Early detection of NSCLC could pave the way for effective therapies. Analysis of molecular genetic biomarkers in biological fluids has been proposed as a useful tool for cancer diagnosis. Here, we aimed to develop a panel of noncoding RNAs (ncRNAs) in sputum for NSCLC early detection. Expression of 11 ncRNAs were analyzed by real–time polymerase chain reaction in sputum samples of 30 NSCLC patients and 30 sex‐ and age‐matched cancer‐free controls. Stability of endogenous microRNAs (miRNAs) in sputum was evaluated after 3 and 6 days at 4°C, 6 months, and 1 year at −80°C. Nine ncRNAs showed significant differences of their expression in sputum between NSCLC patients and controls. A logistic regression model with the best prediction was built based on miR‐145, miR‐126, and miR‐7. The composite of the three miRNAs produced 90% sensitivity and specificity in distinguishing NSCLC patients from the controls. Results indicate that miRNAs could be useful biomarkers based on their stability under various storage conditions and maintain differential changes between cancer and control groups. Moreover, measurement of miRNAs in sputum could be a noninvasive approach for detection of lung cancer.

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VEGF as a potential target in lung cancer

Cited by 176 publications

References 77 publications

Dextrose solution used for prolotherapy decreases cell viability and increases gene expressions of angiogenic and apopitotic factors

Dextrose solution used for prolotherapy decreases cell viability and increases gene expressions of angiogenic and apopitotic factors

Antiangiogenesis Roles of Exosomes with Fei-Liu-Ping Ointment Treatment are Involved in the Lung Carcinoma with the Lewis Xenograft Mouse Model

A panel of noncoding RNAs in non–small‐cell lung cancer

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