Dextrose solutions (in 1%, 5%, 10%, 15%, 20% and 25% concentrations) were applied on adult human fibroblasts in vitro culture condition. Cell proliferation assay was used for finding the cell deaths in cell culture. Up to 80% fibroblast cells died in high dextrose concentrations (15%, 20% and 25%). The deaths of cells were visualized on inverted microscope. In low dextrose concentrations (1%, 5% and 10%), viable cell ratio was above 80%. The gene expression analysis were performed on selected genes which have roles on angiogenesis and apopitosis [VEGFA (Vascular Endothelial Growth Factor A-OMIM ABSTRACTObjectives: Hypertonic dextrose injection in prolotherapy is an injection-based treatment used in chronic musculoskeletal conditions. Dextrose prolotherapy raises growth factor levels and enhances tissue repair, reduces musculoskeletal pain. Despite of uses for many years, the effect of dextrose solution on cellular and molecular base is not fully clear. Here, the roles of dextrose solutions was tried to find out in different concentrations on human fibroblasts in vitro. Gene expression alterations were analyzed in uses of dextrose solutions on growth and apoptotic factors. Methods:The effects of dextrose solution (1%, 5%, 10%-low doses, 15%, 20% and 25%-high doses) were evaluated in vitro by using human fibroblast culture. In each condition total RNA extraction and cDNA synthesis were performed. The gene expression levels of angiogenic and apoptotic factors were analyzed by using real-time polymerase chain reaction. The gene expression results of growth and apoptotic factors were correlated with control results. Results:Results;Dextrose solutions were affected the viability of fibroblast cells in culture flask in high concentrations. In high doses dextrose concentrations, up to 80% of fibroblasts were died because of toxic conditions. Viable fibroblast cell ratios were decreased proportionally due to the dextrose concentration. Low dextrose concentrations increased gene expressions in angiogenic (VEGFA, PDGFA, PDGFB, IGF1) and in apopitotic factors (CASP3 and CASP8) in fibroblasts. Conclusions: Conclusion; Dextrose solution in high concentrations, decreases viable cell ratios on adult fibroblast cell line. Dextrose solutions in proper concentrations increase the gene expressions of angiagenic and apopitotic factors on viable cells in adult fibroblast cell culture.
Angiogenesis is needed for tumor growth and metastasis. For this reason, it represents an exciting target for cancer treatment. Some anti-angiogenic drugs may be useful to prevent angiogenesis during cancer treatment. The aim of this study is to investigate if Ankaferd (standardized herbal extract obtained from five different plants namely Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpina officinarum, and Urtica dioica) have an effect on angiogenesis in chick embryo chorioallontoic membrane. Ankaferd was applied at 1, 5, 20, 50% concentrations to the fertilized eggs on the 6th day. On the 7th and 8th days, all the fertilized eggs were opened and vessels were examined. No significant changes were observed in 1% Ankaferd group and control group. Significant inhibition in angiogenesis was observed in 5, 20, 50% Ankaferd groups. In chick embryo chorioallontoic membrane assay, the anti-angiogenic effect of Ankaferd was shown experimentally for the first time in the literature. The antiangiogenic affect of Ancafert was analyzed on vascular endothelial growth factors (VEGF-A,VEGF-C), and hypoxia-inducible factors (HIF1-A and HIF3-A) genes which have possible roles in angiogenesis on human mesenchimal stem cells and human umbilical vein endothelial cells. Ankaferd treatment increased VEGF-A gene expression levels in HUVECs. VEGF-A gene expression levels remained unchanced in hMSCs with %5 Ankaferd treatment. HIF1-A gene expression levels decreased in HUVECs, whereas increased in hMSCs with 5% Ankaferd treatment. The gene expression levels of HIF3-A increased both in HUVECs and hMSCs. In chick embryo chorioallontoic membrane assay, the anti-angiogenic effect of Ankaferd was shown experimentally for the first time in the literature. These findings may represents the potential uses of Ankaferd in cancer treatment as an anti-angiogenic agent. Due to gene expression analyses resuts in our study, Ankaferd affects the angiogenesis in anti-angiogenic procedure on VEGF and HIF genes.
We aimed to analyze the possible roles of FA and Zn on angiogenesis in vivo culture condition. CAM study revealed that FA and Zn decreased the vessel formation on CAM model. FGF1
Usnic acid is a secondary metabolite in lichens whose role has not been completely elucidated. Lichen extracts containing usnic acid have been utilized in medicine, perfumery, cosmetics, and ecology. Usnic asit inhibits cell growth, induces the cell cycle arrest and apoptosis in human lung carcinoma. Methods:Herein we analyzed the antitumoral effect of usnic acid on the same cell line. We analyzed the gene expression results on APOPT1, CYCS, APAF1, CASP3/9, TNF, BCL2, BCL2L1 and AIFM1 genes which have possible role on cell apopitosis. Results: Usnic acid has stimulatory effect on APOPT1, CYCS, APAF1, CASP3, CASP9 gene expressions in our experiments. Conclusion: Usnic acid has antitumoral effect on cancer cells, affecting on mithocondrial apopitotic genes.
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