Biodiesel derived from plant species has been a major renewable source of energy and has received global interest mainly due to climate change issue. It has increasingly received worldwide attention as a promising alternative fuel. Growing interest in biodiesel production from edible oil brings scarcity in food supply. To overcome this problem, utilization of non-edible oils could be explored. Non-edible oil as biodiesel feedstock impressed in many factors such as energy sustainability and independence in certain areas, especially in rural community, creating job opportunities, elevating environmental merits, and avoiding monoculture of fuel resources. The present chapter reviews several such potentials, including fatty acid methyl ester (FAME) or biodiesel production process of nonedible oil resources as biodiesel feedstock in SouthEast Asian geographical region. The SouthEast Asian countries fall in the tropical region of the world and have many species as non-edible oil, viz., jatropha, karanja, polanga, neem, rubber, and mahua. The oils derived from these species have shown considerable potential as biodiesel feedstock.