Variation and genetic diversity for gliadins in Spanish spelt wheat accessions

Caballero, L.; Martín, L. M.; Álvarez, José Ramón Guzmán

doi:10.1023/b:gres.0000034575.97497.6e

Cited by 32 publications

(22 citation statements)

References 15 publications

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“…Sewa et al (2005) analysing the acid PAGE gliadin patterns of 156 Indian wheats (Triticum aestivum L.), reported extensive polymorphism [genetic diversity index (H) = 0.875] and identified 147 gliadins band patterns. Caballero et al (2004) studied gliadin composition analysis in 403 accessions of Spanish spelt wheat (Triticum aestivum ssp. spelta).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Zaefizadeh

Jamaati-e-Somarin

Ojaghi

et al. 2010

Pesq. agropec. bras.

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-The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

Zaefizadeh

Jamaati-e-Somarin

Ojaghi

et al. 2010

Pesq. agropec. bras.

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“…In addition, the variability found in Indian wheats (Triticum aestivum L.), H = 0.875 (Sewa et al 2005), and in durum wheat landraces in the northwest of Iran and Azerbaijan, H = 0.900 and H = 0.854, respectively (Zaefizadeh et al 2010) was lower than that revealed in the Algerian collection. This variation was also much higher than that of Spanish spelt wheat, H = 0.703 (Caballero et al 2004), Spanish common wheat, H = 0.844 (Metakovsky et al 2000), H = 0.804 (Ruiz et al 2002) and French common wheat, H = 0.714 (Metakovsky & Branlard 1998).…”

Section: Resultsmentioning

confidence: 68%

“…Although care was taken to separate all the Figure 1. Variations detected using Acid-PAGE for some representative samples of Aegilops geniculata; 1: G1, 2: G2, 3: G3, 4: G4, 5: G5, 6: G6, 7: G7, 8: G8, 9: G9, 10: G10, 11: G11, 12: G12, 13: G13, 14: G15, 15: G16 Tanaka et al (2003) and Caballero et al (2004) also reported larger variation in γ-and ω-gliadins than in α-and β-gliadins in Japanese cultivars and in Spanish spelt wheat, respectively. The gene coding for most α and β-gliadins occurs at the Gli-2 loci (Payne 1987); in our study, 35 different combinations were found assuming these two zones together, 34 accessions had a specific combination pattern.…”

Section: Resultsmentioning

confidence: 98%

“…The variation found among the analysed accessions is higher than that found in other materials, thus Harsch et al (1997) found 30 bands in sixteen spelt wheat cultivars. Sewa et al (2005) reported 45 different gliadin bands in 157 Indian wheats, Caballero et al (2004) Diversity index. The genetic diversity (H) based on gliadin patterns, observed in accessions of this species from Algeria (H = 0.959), showed higher polymorphism than in 17 Aegilops cylindrica populations from Iran (H = 0.085) (Khabiri et al 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Gliadins were extracted from a single seed following the procedure of Caballero et al (2004). Electrophoretic separation of gliadins was performed according to the standard Acid-PAGE method described by Caballero et al (2004).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The genetic diversity of gliadins in Aegilops geniculata from Algeria

Medouri¹,

Bellil²,

Khelifi³

2015

CZECH J GENET PLANT

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Medouri A., Bellil I., Khelifi D. (2015): The genetic diversity of gliadins in Aegilops geniculata from Algeria. Czech J. Genet. Plant Breed., 51: 9-15.The gliadins of the wild wheat Aegilops geniculata represent a valuable gene pool in breeding for bread making quality. The genetic diversity of gliadins in A. geniculata was studied among 36 of its accessions, collected in the north of Algeria, using acid polyacrylamide-gel electrophoresis (Acid-PAGE). In total, sixty-one polymorphic bands and 35 gliadin patterns were identified. Twenty-eight different bands and 34 patterns were found in the ω-gliadin region, 13 polymorphic bands and 33 patterns for γ-gliadins, 12 bands and 34 different patterns for β-gliadins and eight bands in combination resulted in 25 different patterns in the α-gliadin zone. Thirty-five patterns were found for each of the Gli-1 (γ/ω region) and Gli-2 (α/β region) loci. The genetic diversity index (H) was higher for ω-gliadins (0.968), followed by γ-and β-gliadins (0.964 and 0.961, respectively), and the lowest value was detected in α-gliadin patterns (0.944). Cluster analysis based on Ward's method divided the analysed collection into five separated groups in which genetic diversity did not follow the geographical distribution. The polymorphism observed in the electrophoretic patterns highlights close correlations between bioclimatic features and some ω-gliadin proteins.

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