The genetic diversity of gliadins in Aegilops geniculata from Algeria

Abstract

Medouri A., Bellil I., Khelifi D. (2015): The genetic diversity of gliadins in Aegilops geniculata from Algeria. Czech J. Genet. Plant Breed., 51: 9-15.The gliadins of the wild wheat Aegilops geniculata represent a valuable gene pool in breeding for bread making quality. The genetic diversity of gliadins in A. geniculata was studied among 36 of its accessions, collected in the north of Algeria, using acid polyacrylamide-gel electrophoresis (Acid-PAGE). In total, sixty-one polymorphic bands and 35 gliadin patterns were identified. Twenty-eight different bands and 34 patterns were found in the ω-gliadin region, 13 polymorphic bands and 33 patterns for γ-gliadins, 12 bands and 34 different patterns for β-gliadins and eight bands in combination resulted in 25 different patterns in the α-gliadin zone. Thirty-five patterns were found for each of the Gli-1 (γ/ω region) and Gli-2 (α/β region) loci. The genetic diversity index (H) was higher for ω-gliadins (0.968), followed by γ-and β-gliadins (0.964 and 0.961, respectively), and the lowest value was detected in α-gliadin patterns (0.944). Cluster analysis based on Ward's method divided the analysed collection into five separated groups in which genetic diversity did not follow the geographical distribution. The polymorphism observed in the electrophoretic patterns highlights close correlations between bioclimatic features and some ω-gliadin proteins.

Karyological study and meiotic analysis of four species ofAegilops(Poaceae) in Algeria

“…The Pearson correlation coefficient showed the influence of environmental factors on karyological characters. Significant correlations were found between other genetic characters of the Aegilops from Algeria, such as the glutenin subunits (Bandou et al 2009) and gliadin subunits (Medouri et al 2015), with environmental conditions.…”

Potential of Aegilops sp. for Improvement of Grain Processing and Nutritional Quality in Wheat (Triticum aestivum)

“…biuncialis (Kozub et al, 2012) and Ae. geniculata (Medouri et al, 2015) have been characterized on the basis of mobility on SDS PAGE (Table 3). Many ω-gliadins have been sequenced and characterized from Ae.…”

Intraspecific divergence of diploid grass Aegilops comosa is associated with structural chromosome changes

“…So far, polymorphism analyses of Aegilops based on gliadin loci were mainly focused on Ae. tauschii Cosson, 1850, the D-genome donor of common wheat (Yan et al 2003;Dudnikov 2018;Badaeva et al 2019a), and only few publications dealt with other Aegilops species (Cole et al 1981;Medouri et al 2015;Garg et al 2016). On the other hand, owing to similar structure of gliadin loci in wheat and Aegilops (Dong et al 2016;Huo et al 2018) and extremely high polymorphism, gliadins can serve as supplementary markers for the assessing genetic variability of Ae.…”