Variation and Divergence of Multilocus Genome Markers in the Species of the Genus Chironomus (Diptera, Chironomidae)

Гундерина, Л. И.; Kiknadze, I. I.; Istomina, A. G.; Golygina, Veronika V.

doi:10.1007/s11177-006-0006-7

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…Thus, the use of another nuclear gene might be more appropriate for Chironomus species identifications. Other studies have used the nuclear internal transcribed spacer (ITS) region to separate C. plumosus from C. entis as well as other Chironomus species (Gunderina & Katokhin 2011;Martin 2011;Gunderina 2012). The nuclear carbamoylphosphate synthetase (CAD) region has also been successfully used to identify chironomid species (Carew et al 2011).…”

Section: Approaches Used To Delimit Chironomus Speciesmentioning

confidence: 99%

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Proulx

Martin

Carew

et al. 2013

Zootaxa

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Chironomus Meigen (Diptera, Chironomidae) larvae are usually the largest sediment-burrowing chironomids, and as such often constitute a major part of the freshwater infaunal biomass. However, use of this genus in ecological, environmental and paleoecological studies is hampered by the fact that Chironomus larvae are difficult to identify to species because the larvae of many species are morphologically similar. We used a combination of morphological, cytological and genetic techniques to distinguish Chironomus larvae collected from 31 water bodies located in eastern Canada, producing 17 distinguishable groupings. These groups of larvae were ultimately identified as belonging to 14 known species (C. anthracinus, C. bifurcatus, C. cucini, C. decorus-group sp. 2, C. dilutus, C. entis, C. frommeri, C. harpi, C. maturus, C. nr. atroviridis (sp. 2i), C. ochreatus, C. plumosus, C. staegeri and C. 'tigris') and three other species that remain unidentified (C. sp. NAI-III). No single approach served to delimit and identify larvae of all 17 Chironomus species that we collected. Although we expected that morphological criteria alone would be insufficient, our results suggest that DNA barcoding, using either the mitochondrial cox1 or the nuclear gb2β gene, was also inadequate for separating some Chironomus species. Thus we suggest that multiple approaches will often be needed to correctly identify Chironomus larvae to species.

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Section: Approaches Used To Delimit Chironomus Speciesmentioning

confidence: 99%

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Proulx

Martin

Carew

et al. 2013

Zootaxa

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“…In spite of relative geographic proximity, the genetic distances between Caucasian populations are quite large (Table 6), and they do not form a single cluster of Caucasian populations. The distance value between populations of Central and South Caucasus (0.3853) does not exceed the distance range (0.136–0.474) for different population of the one species (Gunderina 2001). At the same time, the distance value between populations of Central Caucasus and Eastern Ciscaucasia (0.5318) in one hand and the distance value between populations of Eastern Ciscaucasia and South Caucasus (0.8232) in other hand exceeds those ranges and fall in the distance range (0.474–2.815) for different subspecies (Gunderina 2001).…”

Section: Resultsmentioning

confidence: 83%

“…The distance value between populations of Central and South Caucasus (0.3853) does not exceed the distance range (0.136–0.474) for different population of the one species (Gunderina 2001). At the same time, the distance value between populations of Central Caucasus and Eastern Ciscaucasia (0.5318) in one hand and the distance value between populations of Eastern Ciscaucasia and South Caucasus (0.8232) in other hand exceeds those ranges and fall in the distance range (0.474–2.815) for different subspecies (Gunderina 2001). One can see that separation of the population of Eastern Ciscaucasia from other Caucasian populations is relatively big and even reaches a level of subspecies.…”

Section: Resultsmentioning

confidence: 83%

Karyotype characteristics and chromosomal polymorphism of Chironomus “annularius” sensu Strenzke (1959) (Diptera, Chironomidae) from the Caucasus region

Karmokov¹

2018

CCG

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The study presents data on the karyotype characteristics and features of chromosomal polymorphism of Chironomus “annularius” sensu Strenzke (1959) (Diptera, Chironomidae) from three populations of the Caucasus region (South and Central Caucasus, and Eastern Ciscaucasia). We found 17 banding sequences in the Caucasian populations. We observed inversion polymorphism in almost all chromosome arms except for arm G. The genetic distances between all the studied populations of Ch. “annularius” were calculated using Nei criteria (1972). In spite of relative geographic proximity, the genetic distances between populations of the Caucasus are quite large, and they do not form a single cluster of Caucasian populations. The population of the South Caucasus goes to the European cluster, the population of the Central Caucasus goes to the Asian cluster and the population of Eastern Ciscaucasia does not belong to any of the outlined clusters. Principal component analysis (PCA) shows a similar picture. Two of the Caucasian populations do not follow Hardy-Weinberg expectation, there being a marked deficiency of heterozygotes in arms A, B and C, arguably, due to negative selection of heterozygotes or founder effect. All the obtained data are indicative of the complex genetic structure of Caucasian populations of Ch. “annularius” and total complexity microevolution processes occurring in the Caucasus region.

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“…A positive control (C+) was used to guarantee that a negative amplification result was determined by the absence of the sequence corresponding to the primer binding site rather than an unsuccessful PCR determined, for example, by a poor DNA quality or any other reasons. PCR with the primer pair 5'-GTAACAAGGTTTCCGTAGG-3' (chir5F) and 5'-CGACACTCAACCATATGTACC-3' (chir5R), giving a full-sized ITS1-5.8S rDNA fragment with a length of ~480 bp (Gunderina & Katokhin 2011), was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…The ITS1 nucleotide sequences of chironomids are highly polymorphic, while 5.8S rDNA are highly conserved. Extended species-specific sequences with their length sufficient for constructing primers have been found in the ITS1 region (Gunderina & Katokhin 2011). Correspondingly, ITS1 and 5.8S rDNA nucleotide sequences were used to design the speciesspecific primers for C. plumosus and C. balatonicus.…”

Section: Introductionmentioning

confidence: 99%

Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera)

Гундерина

2012

Fauna Norv.

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Species-specific PCr primers for identification of the sibling species Chironomus plumosus (linnaeus, 758) and Chironomus balatonicus (devai, wuelker et Scholl, 83) (Chironomidae, diptera) larissa I. gunderina Gunderina LI. 2012. Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera). Fauna norvegica 31: 151-157. Applicability of the polymerase chain reaction (PCR) with species-specific primers to obtaining molecular markers for identification of the sibling species of Chironomus plumosus group-C. plumosus and C. balatonicus-has been estimated. The nucleotide sequences of internal transcribed spacer (ITS) from the locus encoding ribosomal RNA (rRNA) were used as the source for designing the species-specific primers. The primers allowing for identification of C. plumosus and C. balatonicus were constructed. One primer pair (plu107F/plu363R) gives the PCR product MAR2, specific of C. plumosus, and the other (bal86F/plu363R), the PCR product MAR6, specific of C. balatonicus. The testing involving 18 species of the genus Chironomus confirmed the specificity of the primers. The results suggest that the PCR with species-specific primers is promising for construction of molecular markers for identification not only of these two, but also of other Chironomus species.

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Variation and Divergence of Multilocus Genome Markers in the Species of the Genus Chironomus (Diptera, Chironomidae)

Cited by 9 publications

References 15 publications

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Karyotype characteristics and chromosomal polymorphism of Chironomus “annularius” sensu Strenzke (1959) (Diptera, Chironomidae) from the Caucasus region

Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera)

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