Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera)

Гундерина, Л. И.

doi:10.5324/fn.v31i0.1381

“…Thus, the use of another nuclear gene might be more appropriate for Chironomus species identifications. Other studies have used the nuclear internal transcribed spacer (ITS) region to separate C. plumosus from C. entis as well as other Chironomus species (Gunderina & Katokhin 2011;Martin 2011;Gunderina 2012). The nuclear carbamoylphosphate synthetase (CAD) region has also been successfully used to identify chironomid species (Carew et al 2011).…”

Section: Approaches Used To Delimit Chironomus Speciesmentioning

confidence: 99%

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Proulx

¹

,

Martin

²

,

Carew

³

et al. 2013

Zootaxa

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Chironomus Meigen (Diptera, Chironomidae) larvae are usually the largest sediment-burrowing chironomids, and as such often constitute a major part of the freshwater infaunal biomass. However, use of this genus in ecological, environmental and paleoecological studies is hampered by the fact that Chironomus larvae are difficult to identify to species because the larvae of many species are morphologically similar. We used a combination of morphological, cytological and genetic techniques to distinguish Chironomus larvae collected from 31 water bodies located in eastern Canada, producing 17 distinguishable groupings. These groups of larvae were ultimately identified as belonging to 14 known species (C. anthracinus, C. bifurcatus, C. cucini, C. decorus-group sp. 2, C. dilutus, C. entis, C. frommeri, C. harpi, C. maturus, C. nr. atroviridis (sp. 2i), C. ochreatus, C. plumosus, C. staegeri and C. 'tigris') and three other species that remain unidentified (C. sp. NAI-III). No single approach served to delimit and identify larvae of all 17 Chironomus species that we collected. Although we expected that morphological criteria alone would be insufficient, our results suggest that DNA barcoding, using either the mitochondrial cox1 or the nuclear gb2β gene, was also inadequate for separating some Chironomus species. Thus we suggest that multiple approaches will often be needed to correctly identify Chironomus larvae to species.

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“…Thus, the use of another nuclear gene might be more appropriate for Chironomus species identifications. Other studies have used the nuclear internal transcribed spacer (ITS) region to separate C. plumosus from C. entis as well as other Chironomus species (Gunderina & Katokhin 2011;Martin 2011;Gunderina 2012). The nuclear carbamoylphosphate synthetase (CAD) region has also been successfully used to identify chironomid species (Carew et al 2011).…”

Section: Approaches Used To Delimit Chironomus Speciesmentioning

confidence: 99%

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

PROULX¹,

Martin

²

,

Carew

³

et al. 2013

Zootaxa

View full text Add to dashboard Cite

Chironomus Meigen (Diptera, Chironomidae) larvae are usually the largest sediment-burrowing chironomids, and as such often constitute a major part of the freshwater infaunal biomass. However, use of this genus in ecological, environmental and paleoecological studies is hampered by the fact that Chironomus larvae are difficult to identify to species because the larvae of many species are morphologically similar. We used a combination of morphological, cytological and genetic techniques to distinguish Chironomus larvae collected from 31 water bodies located in eastern Canada, producing 17 distinguishable groupings. These groups of larvae were ultimately identified as belonging to 14 known species (C. anthraci-

show abstract

“…The results showed that the studied taxon is the species complex. Usually, species and larval forms are confined to the type of water bodies or specific environmental conditions [43][44][45]. In reviewing the literature, Spies and Saether [1] found that larvae of C. annularius sensu lato are found in water bodies of various types, including small freshwater bodies, the profundal zone of lakes, and saline waters such as the Baltic Sea littoral, and are widely spread geographically; it could be that different species were identified as C. "annularius".…”

Section: Discussionmentioning

confidence: 99%

“…In reviewing the literature, Spies and Saether [1] found that larvae of C. annularius sensu lato are found in water bodies of various types, including small freshwater bodies, the profundal zone of lakes, and saline waters such as the Baltic Sea littoral, and are widely spread geographically; it could be that different species were identified as C. "annularius". It is known that Chironomus has high variability at the chromosomal and biochemical levels of organization, allowing it to inhabit various types of water bodies [8,43,[46][47][48]. The cytogenetic distances between two distant reservoirs (for example lakes with similar conditions) can be minimal, and vice versa, the distances between two neighboring reservoirs (for example a lake and a river) can be very large, with karyoforms even arising [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Karyotypes and COI Gene Sequences of Chironomus annularius Sensu Lato (Diptera, Chironomidae) from Russia, Mongolia, and Armenia

Bolshakov,

Prokin,

Mayor

et al. 2023

Water

1

0

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The larvae of the genus Chironomus are a common object for hydrobiological studies, as well as a model object for cytogenetics. Morphologically, the species are very similar. One of these species or species complex is Chironomus “annularius”, which has a Holarctic distribution. It has chromosomal banding sequences characteristic of Nearctic and Palearctic populations. Using an integrated method that included morphology, cytogenetics, and molecular genetics, we analyzed populations from Russia, Mongolia, and Armenia. We found through cytogenetics and larval morphology that the populations have high similarity. Molecular genetic studies have shown significant differences between the populations. The genetic distances between the populations, in some cases, exceed the interspecific threshold of 3%, and are 6.5%. In the South Caucasian population (Lake Sevan), a chromosomal banding sequence, h’annD3, that was previously observed only in North America, was found for the first time. The larvae from Lake Sevan have large genetic distances from others, and are morphologically similar to the species Chironomus markosjani Shilova 1983, described from this lake without comparison with Ch. annularius nor an exact description of the karyotype. The sequences of the COI genes from Montenegro (Lake Skadar) and West Siberia (Novosibirsk) found in GenBank may belong to a new undescribed species, or a species not represented in the database. Thus, the analyzed data on Chironomus “annularius” support the presence of the complex of homosequential species under this name.

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Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera)

Cited by 6 publications

References 11 publications

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

Using various lines of evidence to identify Chironomus species (Diptera: Chironomidae) in eastern Canadian lakes

<p class="HeadingRunIn"><strong>Using various lines of evidence to identify <em>Chironomus</em> species (Diptera: Chironomidae) in eastern Canadian lakes</strong></p>

Karyotypes and COI Gene Sequences of Chironomus annularius Sensu Lato (Diptera, Chironomidae) from Russia, Mongolia, and Armenia

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