Variación somaclonal y selección in vitro con toxinas como herramienta en la búsqueda de resistencia a enfermedades en plantas: Revisión

Torres, Carlos Patiño

doi:10.22490/21456453.893

Cited by 2 publications

(1 citation statement)

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“…These include supplementing culture media with growth retardants, osmotic inhibitors or tissue dehydrators, and using lower temperatures or lower light conditions (Engelmann 1997;Daniells et al 2001;Buitink et al 2002;Engelmann 2004;Benson 2008;Reed 2008). However, this conservation strategy has been questioned, as it can lead to long-term genetic instability (Roca et al 1991;Patiño Torres 2010) and morphogenetic abnormalities (Adams et al 1999;Dumet & Benson 2000). In Musa, these problems have been overcome by cryopreserving apical meristems, a process that is generally performed by incorporating the tissue in the cryogenic process under conditions of dehydrating and cooling (Esterbauer et al 1988;Panis & Swennen 1995).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Proliferation and Cryoconservation of Banana and Plantain Elite Clones

Reyes

Garcı́a

Piña

et al. 2017

Journal of Horticultural Research

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Agriculture and modern biotechnology are increasingly becoming interdependent, and many new techniques have brought new opportunities for enhancing production and marketing. Germplasm storage is an alternative for the conservation of plant genetic diversity, contributing to the improvement and maintenance of propagation programs for species of interest. In this work, banana corms were collected as plant material from relatively young commercial plantations of three different cultivars: 'Williams', Valery (AAA genome; Cavendish subgroup), and 'Barraganete' (AAB genome; Plantain subgroup). Their shoot tips were introduced into in vitro conditions, and subcultured monthly to obtain the required number of shoots. The shoots were subsequently rooted and stimulated to invigoration in order to extract apical meristems (0.8-1.0 mm), which were prepared for cryopreservion in liquid nitrogen (−196 °C) following preconditioning in PVS2 vitrification solution. Thereafter, the explants were rapidly thawed and then recovered and regenerated using two different methods -by Panis (2009) and Korneva et al. (2009) -consisting of two different sets of recovery and subsequent regeneration media. Statistical analysis of the results showed that the banana cultivar 'Williams' demonstrated higher survival and regeneration rates after cryopreservation using the Korneva method, whereas in cultivars 'Valery' and 'Barraganete', there were no significant differences between the tested methods. The 'Barraganete' cultivar had the lowest survival and regeneration rates, regardless of the applied method.

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