No abstract

No abstract

The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each other.

No abstract

El presente estudio evaluó el efecto de diferentes concentraciones y tiempo de exposición de colchicina, sobre la morfología foliar y los estomas de Kalanchoe daigremontiana. Inicialmente, se recolectaron y se sometieron las plántulas de K. daigremontiana a concentraciones de 0,025% y 0,1% (p/v) de colchicina y a dos tiempos de exposición (24 y 48 horas). Posteriormente, se realizaron estudios morfológicos como la altura de la planta (AP), anchura de la hoja (AH), número de hojas (NH), longitud foliar (LH), espesor de hoja (EH) y volumen foliar (VH) cada 15 días, durante 16 semanas después de la siembra. Luego, se caracterizaron los estomas, teniendo en cuenta el ancho, el largo, índice estomático y el número de cloroplasto por estoma. Se encontró un incremento significativo en la morfología foliar y estomática, en los tratamientos con colchicina de 0,025% a 24 y 48 horas. Lo que demuestra que el uso de la colchicina logra un mayor crecimiento en poco tiempo y aumento de la biomasa en la planta medicinal K. daigremontiana.

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

No abstract

No abstract