The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each other.
<p class="042abstractstekst">This research attempted to determine the efficacy of the tetrazolium test in the evaluation of the seed viability of two varieties of <em>Coffea arabica</em> L. (‘Castillo’ and ‘Cenicafé’). The fruits were obtained from crops located in the municipalities of Salazar de las Palmas and Arboledas (Norte de Santander - Colombia). The test was carried out with embryos manually extracted from the seeds using tweezers. Three pretreatments were established: distilled water, sodium hypochlorite (2.5 %), sucrose (10 %), and a control (no pretreatment). Embryos were placed in a cysteine solution (0.5 %) to prevent oxidation, then immersed in tetrazolium solutions with concentrations of 0.035 %, 0.075 %, and 0.1 % for a period of 6, 9, and 12 hours in darkness. The results of the viability test were validated with seed germination, using the wet paper towel method in darkness. The best viability percentages were found with the application of sodium hypochlorite (NaClO 2.5 %), with a high correlation with the germination percentage. The use of pretreatments improved the efficiency of the viability test and allowed the use of low concentrations of the reagent (0.035 %), giving the farmer a quick and less expensive alternative to determine germination capacity.</p>
Las bacterias diazotróficas pueden estimular el crecimiento del cultivo del tomate (Lycopersicon esculentum Mill.) mediante la síntesis de hormonas, fijación de N y producción de sideróforos, u otros procesos. Las bacterias diazotróficas predominantes en los agroecosistemas pueden ser biofertilizantes potenciales. El objetivo del estudio fue cuantificar y caracterizar la población de bacterias diazotróficas rizosféricas en muestras de suelo rizosférico (SR) y bacterias diazotróficas endófitas en muestras de raíces (R) y material foliar (MF), mediante pruebas fenotípicas y moleculares. El estudio fue exploratorio y con un muestreo aleatorio, con 18 muestras SR, raíces (R) y MF de cultivos de tomate en seis fincas. Las diferencias en el número más probable (NMP) de bacterias diazotróficas no fueron significativas entre los sitios de las muestras de MF y R. La media de NFb mostró diferencias altamente significativas en Azotobacter sp. y Azospirillum sp. entre las muestras de SR, R y MF, con un NMP mayor y menor en SR y MF. Esto se relaciona con la caracterización fenotípica y bioquímica de SR donde se identificaron 14 aislados como Azotobacter sp. y siete como Azospirillum sp. Los géneros Burkholderia y Gluconacetobacter no mostraron diferencias significativas en el número de aislados entre muestras de SR y R, pero sí respecto a MF, con la población celular menor. Las diferencias entre las fincas no fueron significativas en las variables del suelo relacionadas con la disimilitud de NMP. La caracterización molecular permitió identificar A. chroococcum, A. nigricans, A. vinelandii, A. brasilense, B. glumae y G. azotocaptans/G. johannae en muestras de SR; B. glumae, G. azotocaptans/G. johannae en muestras de R y G. azotocaptans/G. johannae en muestras de MF. Palabras claves: diazotróficas, endófitas, rizosféricas, Lycopersicon esculentum Norte de Santander.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.