In Vitro Proliferation and Cryoconservation of Banana and Plantain Elite Clones

Abstract

Agriculture and modern biotechnology are increasingly becoming interdependent, and many new techniques have brought new opportunities for enhancing production and marketing. Germplasm storage is an alternative for the conservation of plant genetic diversity, contributing to the improvement and maintenance of propagation programs for species of interest. In this work, banana corms were collected as plant material from relatively young commercial plantations of three different cultivars: 'Williams', Valery (AAA genome; Cavendish subgroup), and 'Barraganete' (AAB genome; Plantain subgroup). Their shoot tips were introduced into in vitro conditions, and subcultured monthly to obtain the required number of shoots. The shoots were subsequently rooted and stimulated to invigoration in order to extract apical meristems (0.8-1.0 mm), which were prepared for cryopreservion in liquid nitrogen (−196 °C) following preconditioning in PVS2 vitrification solution. Thereafter, the explants were rapidly thawed and then recovered and regenerated using two different methods -by Panis (2009) and Korneva et al. (2009) -consisting of two different sets of recovery and subsequent regeneration media. Statistical analysis of the results showed that the banana cultivar 'Williams' demonstrated higher survival and regeneration rates after cryopreservation using the Korneva method, whereas in cultivars 'Valery' and 'Barraganete', there were no significant differences between the tested methods. The 'Barraganete' cultivar had the lowest survival and regeneration rates, regardless of the applied method.

Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture

“…The number of proliferating buds increased by the fifth subculture with the number of buds more than doubled in Litete (11.2) while the number of buds for Libanga Likale increased less drastically (8.6). Reyes et al (2017) found in vitro proliferation rates of 1.95-2.20 in plantains, while Korneva et al (2013) found a 0.8 proliferation rate for plantain and 1.86 for banana. Our results are also in line with Dhed'a (1992), who showed that 10 μM BAP increased the proliferation rates in in vitro with 3.5 for the plantain cultivar Three Hand Planty (Musa AAB) with 18.6, 12.4 and 21.4 for the cooking banana cultivars Bluggoe, Cardaba and Saba (Musa ABB) and 7.1 for the dessert banana cutivar Yangambi Km5 (Musa AAA).…”

In Vitro Medium-Term Storage of Banana Cultivar ‘Barangan’ Using Alginate-Encapsulation Technique

“…In vitro storage with minimal growth by encapsulation using sodium alginate in Murashige and Skoogs Medium is necessary to reduce the constraints of banana preservation. Sodium alginate and calcium chloride are the best combinations for encapsulation explants because these ions are non-destructive, inexpensive, easy to use, and result in the high conversion of encapsulated embryos to plants (Sharma et al 2012a;Reyes et al 2017;Phanomchai et al 2022). It is also cost-effective for clonal propagation systems (Ahmad et al 2012).…”