Variability in the determination of ash free dry weight for periphyton communities: A call for a standard method

Ridley-Thomas, Christopher I; Austin, A. P.; Lucey, W. P.; Clark, Malcolm

doi:10.1016/0043-1354(89)90197-8

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…The organic matter content is commonly estimated as ash-free dry mass (AFDM). The methodologies for these analyses are well established, although, particularly in the case of chlorophyll, there is controversy about the best procedure (Nusch 1980;Sartory and Grobbelaar 1984;Ridley-Thomas et al 1989;Steinman and Lamberti 1996). Periphyton analyses are routinely made in environmental assessments, studies of stream communities and ecosystems and research on periphyton itself.…”

Section: Introductionmentioning

confidence: 99%

Patterns of periphyton chlorophyll and dry mass in a neotropical stream: a cheap and rapid analysis using a hand-held fluorometer

Moulton

Souza²,

Walter

et al. 2009

Mar. Freshwater Res.

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Periphyton distribution is affected by various physical, chemical and biological factors at different scales. In the present study, a cheap and rapid method of measurement was used to obtain the many samples necessary for spatial analysis of periphyton along an altitudinal gradient in a low-order stream in south-east Brazilian Atlantic rain-forest. Fluorescence and turbidity were measured using a hand-held fluorometer and calibrated to the chlorophyll and dry mass of periphyton. Periphyton on the horizontal and vertical surfaces of different boulders was examined and shading and water current were measured as covariables. The three upstream sites with higher abundances of potentially grazing and bioturbing shrimps and mayflies had significantly less periphyton dry mass than the three downstream sites. Chlorophyll was positively related to water current, but not to shading. Variabilities in the dry mass and chlorophyll among boulders within sites were not associated with the distribution of shrimps and mayflies. The in vivo measurement was cheap, rapid, sensitive and reasonably precise compared with standard methods. The necessary sacrifice of detail of pigments (different chlorophylls and pheophytin) and dry mass (organic and inorganic constituents) and probably precision was compensated for by the insights gained from the ability to obtain a large number of samples in a hierarchical design.

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Section: Introductionmentioning

confidence: 99%

Patterns of periphyton chlorophyll and dry mass in a neotropical stream: a cheap and rapid analysis using a hand-held fluorometer

Moulton

Souza²,

Walter

et al. 2009

Mar. Freshwater Res.

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“…The determined dry weigh is based on ash‐free basis: the watery content that was removed at 105°C and volatile content that was removed inside the furnace at 550°C. The difference between these two amounts is the reported dry weight 71 …”

Section: Methodsmentioning

confidence: 99%

“…69,70 For dry weight measurement, a sample of 50 mL of microalgae culture was filtered through cellulose acetate 0.45-μm membrane filters (Sartorius AG, Germany), then dried at 105 • C for 12 h and 550 • C for 6 h. 27,64 The determined dry weigh is based on ash-free basis: the watery content that was removed at 105 • C and volatile content that was removed inside the furnace at 550 • C. The difference between these two amounts is the reported dry weight. 71…”

Section: Microalgae Cultivationmentioning

confidence: 99%

Flocculation of Chlorella vulgaris with alum and pH adjustment

Mohseni

Zenooz

2021

Biotech and App Biochem

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Microalgae, a group of photosynthetic microorganisms, are a promising feedstock for biodiesel production, but their biomass retrieval is a challenging task.Flocculation is a feasible method for dewatering and harvesting microalgae biomass. In the current study, the effect of alum flocculation on Chlorella vulgaris biomass retrieval has been studied. Alum structural changes with pH were led to a full factorial design to address the effect of this chemical structure changes at different pH values. It is observed that the best flocculation efficiency could be achieved in the natural pH value of C. vulgaris growth medium (8.2) with less than 0.5 g/L flocculant addition, which would lead to the flocculation efficiency of more than 90%. An ensemble architecture of neural networks successfully employed for flocculation modeling.

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An assessment of recent trophic changes in Windermere South Basin (England) based on diatom remains and fossil pigments

Sabater¹,

Haworth

1995

J Paleolimnol

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Variability in the determination of ash free dry weight for periphyton communities: A call for a standard method

Cited by 5 publications

References 14 publications

Patterns of periphyton chlorophyll and dry mass in a neotropical stream: a cheap and rapid analysis using a hand-held fluorometer

Patterns of periphyton chlorophyll and dry mass in a neotropical stream: a cheap and rapid analysis using a hand-held fluorometer

Flocculation of Chlorella vulgaris with alum and pH adjustment

An assessment of recent trophic changes in Windermere South Basin (England) based on diatom remains and fossil pigments

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