Variability in intra-specific and monosporous isolates of Volvariella volvacea based on enzyme activity, ITS and RAPD

Ahlawat, O P; Gupta, Priyanka; Kamal, Shwet; Dhar, B. L.

doi:10.1007/s12088-010-0031-z

Cited by 4 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amount of protease was determined by the Folin phenol method described by Johnston et al (1998). Laccase (EC 1.10.3.2) assay was conducted according to the method described by Ahlawat et al (2010), and its activity was calculated in terms of the change in absorbance by 0.001 per min per mL of enzyme at 25°C. Lipase (EC 3.1.1.3) was determined by a base titration method described by Brockman (1981).…”

Section: Enzyme Assaymentioning

confidence: 99%

Effects of fermentation by Ganoderma lucidum and Saccharomyces cerevisiae on rape pollen morphology and its wall

et al. 2017

View full text Add to dashboard Cite

This study investigated the mechanism of wall rupture fermentation of the rape bee pollen by and. The enzymatic activities and broken-wall ratios were determined, and the results suggested the involvement of cellulase, protease, and pectinase in the wall rupture fermentation. Among the five substrate inducers, CMCase, protease, and pectinase had the highest enzymatic activities at 23.13 ± 1.09, 63.44 ± 1.35, and 118.61 ± 2.07 U/mL, respectively. The broken-wall ratios of and were 85.08 ± 3.92 and 88.31 ± 2.51%, respectively. The wall rupture of rape pollen was closely related to the enzymes of the fermenting species. The wall rupture process was determined through enzyme assay, light microscopy, and scanning electron microscopy, and the broken-wall ratios provide further information on the said mechanism. The process follows three steps, namely, removal of coating on the pollen surface, continuous opening of three germinal apertures to allow enzymatic breakdown of the intine, and release of contents from the degradation of the exine.

show abstract

Section: Enzyme Assaymentioning

confidence: 99%

Effects of fermentation by Ganoderma lucidum and Saccharomyces cerevisiae on rape pollen morphology and its wall

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Volvariella volvacea, commonly known as straw mushroom, has become one of the most important cultivated mushrooms in the world due to its pleasant taste, distinct flavour, high content of protein, and short cultivation period of approximately two weeks (Rajapakse, 2011;Thiribhuvanamala et al, 2012;Thakur and Singh, 2014). However, its yields are much lower as compared to other mushrooms (Ahlawat et al, 2010). It requires a high temperature range within 30 -37°C for hyphal development and fructification (Rajapakse, 2011;Roy et al, 2014;Thakur and Singh, 2014).…”

Section: Introductionmentioning

confidence: 99%

Physical quality, amino acid contents, and health risk assessment of straw mushroom (Volvariella volvacea) at different maturity stages

Mohd Joha,

Misran,

Mahmud

et al. 2021

IFRJ

View full text Add to dashboard Cite

Volvariella volvacea, also known as straw mushroom, is an edible mushroom, and a highly nutritious food source. In Malaysia, tremendous increase in the consumption of this mushroom is due to its characteristics; short cropping duration, distinct flavour, and pleasant taste. During harvesting, the maturity of V. volvacea is vital to obtain high quality produce. As the mature stage quickly succeeds the immature stage, farmers usually collect both the immature and mature stages at harvest. Thus, the objective of the present work was to evaluate the physical quality, nutrients, and health risk assessment of V. volvacea at both maturity stages. The mushrooms were cultivated on composted empty fruit bunch (EFB), and harvested after a week at immature (button) and mature (veil opening) stages. The absence of spores at the button stage could lower the metabolic activity after harvest, thus decelerating the deterioration rate. Button stage mushrooms showed significantly higher firmness and higher content of glutamic acid. In term of safety, both stages were within the dietary intake limit for heavy metals. In conclusion, the button stage could be suitable and practical for consumption and commercialisation.

show abstract

“…[7,9] were used in the present study. Similarly, 4 single spore isolates (OE-12-06, OE-12-22, OE-55-08 and OE-55-30), 2 each from parent strains, OE-12 and OE-55, respectively, isolated by following the standard method [8] and pre-screened for mycelial growth characteristics, extracellular lignocellulolytic enzymes activity profiles and genetic distinctness [10] were used in the present study. The cultures were multiplied and maintained on Malt Extract Agar (MEA) medium slants at 32 ± 2 and 18 ± 2°C, respectively in BOD incubator.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Volvariella volvacea Strains for Yield and Diseases/Insect-Pests Resistance Using Composted Substrate of Paddy Straw and Cotton Mill Wastes

Ahlawat¹,

Singh²,

Kumar³

2011

Indian J Microbiol

Self Cite

View full text Add to dashboard Cite

Out of the 3 parent strains and 4 single spore isolates of Volvariella volvacea evaluated, strain, OE-274 gave earliest yield in 11.25-11.50 days post-spawning in all 4 trials. The yield varied in different strains in different trials and it was highest in strain, OE-272 in trial 1, SSI, OE-55-08 in trial 2, and strain, OE-274 in trial 3 and 4. In overall average, highest yield was in strain, OE-272, closely followed by strain, OE-274. The number of fruiting bodies per q substrate also varied in different strains in different trials. Highest numbers were in strain, OE-272, SSIs, OE-55-08 and OE-12-22, and strain, OE-210 in trial 1, 2, 3 and 4, respectively. Highest fruiting body wt was in strain, OE-274 in all 4 trials. The yield during different weeks of cropping varied in different strains but invariably it was highest in first week, which accounted for 60-70% of the total yield. The fruiting bodies of strain, OE-274 were of bigger size, brownish, toughest and with least tendency of veil opening, while that of strain, OE-272 and SSI, OE-55-08 were whitish to grayish-white, oblong, medium size, delicate and lesser tendency of veil opening. The strain, OE-274 and SSI, OE-55-08 exhibited higher resistance against the growth of competitor moulds and infestations of insect-pests, while strain, OE-272 exhibited highest susceptibility to insect-pests infestation.

show abstract

Variability in intra-specific and monosporous isolates of Volvariella volvacea based on enzyme activity, ITS and RAPD

Cited by 4 publications

References 8 publications

Effects of fermentation by Ganoderma lucidum and Saccharomyces cerevisiae on rape pollen morphology and its wall

Effects of fermentation by Ganoderma lucidum and Saccharomyces cerevisiae on rape pollen morphology and its wall

Physical quality, amino acid contents, and health risk assessment of straw mushroom (Volvariella volvacea) at different maturity stages

Evaluation of Volvariella volvacea Strains for Yield and Diseases/Insect-Pests Resistance Using Composted Substrate of Paddy Straw and Cotton Mill Wastes

Contact Info

Product

Resources

About