Human dietary deficiency is one the dismaying question due to consumption of modern readymade foods in today's hectic life pattern. Broad spectrums of population are enforcing them toward malnutrition or nutritional deficiency in developing or underdeveloped countries. Additional nutritious and balanced food sources must be made available to control this present scenario. However, conventional protein-rich resources would not compensate the demand of protein-rich food crops with increase in raising population. In this context, algae could be the potent higher proteinrich food supplements through carbon allocation to algal biomass generation without hampering food chain, and soil resources depletion. Realizing the wide-scale adoption, high protein yielding algal isolates with lowered nucleic acid content and higher chlorophyll content need to be explored considering its toxicity on community health. To this end, the diverse topographical locations of the Kangsabati River have been considered for isolation of broad spectrum of algal regimes to examine quantity of total protein, nucleic acid, and chlorophylls. The present study has depicted that algal isolate DK1 contains highest protein and chlorophyll content in the best on the 12 th day indulging 36% protein and 13% chlorophyll where total nucleic acid amount is very limiting 0.52%. Moreover, a DK1 alga has shown limited pathogenicity through hemolysin assay toward its acceptance for community usage as single-cell protein-rich food supplement and alterative nutraceutical.