Value of a spectroscopic "fecalogram" in determining the etiology of steatorrhea.

Peuchant, Evelyne; Salles, Christine; Jensen, Raymond

doi:10.1093/clinchem/34.1.5

Cited by 28 publications

(3 citation statements)

References 5 publications

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“…Although our results are comparable to those of other groups (7‐10) we determined other optimal wavelength combinations. A different stool matrix related to different eating habits or different analytical instruments (that is, with other detector characteristic curves) are possible reasons for these differing wavelength combinations.…”

Section: Discussionsupporting

confidence: 81%

Simultaneous Determination of Fecal Fat, Nitrogen, and Water by Near‐Infrared Reflectance Spectroscopy

Neumeister,

Henker,

Kaltenborn

et al. 1997

J. pediatr. gastroenterol. nutr.

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BackgroundDeterminations of fecal fat and nitrogen reveal evidence of malabsorption and assist in estimating the efficacy of pancreatic enzyme treatment. Seventy‐two‐hour stool collection, with chemical analysis of fecal fat, and Kjeldahl's method for measurement of fecal nitrogen are generally accepted as standard methods for making these determinations. However, these traditional methods are expensive, timeconsuming, and cumbersome. This study evaluated the efficiency and validity of an alternative method, using nearinfrared reflectance spectroscopy (NIRS) and compared results with those of the standard methods.MethodsNear‐infrared reflectance spectroscopy is a secondary method: The instrument first has to be calibrated with samples analyzed by the standard method. Sixty‐three stool samples with known fat content (range 4.79‐292.5 mg/g), 24 samples with known nitrogen content (range 5.36‐19.38 mg/g), and 24 samples with known water concentration (range 60.1‐82.22%) served for calibration. A further 69 samples were analyzed to validate the procedure.ResultsThere was a satisfactory correlation between the measurements produced by near‐infrared reflectance spectroscopy and those produced by standard methods: fat r = 0.97; nitrogen r = 0.94; water r = 0.96.ConclusionsNear‐infrared reflectance spectroscopy appears to be a reliable, simple, and rapid method of measuring different fecal components‐as precise and accurate as the standard methods. Stool samples should be analyzed immediately after collecting or stored only a few days before analyzing.

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Section: Discussionsupporting

confidence: 81%

Simultaneous Determination of Fecal Fat, Nitrogen, and Water by Near‐Infrared Reflectance Spectroscopy

Neumeister,

Henker,

Kaltenborn

et al. 1997

J. pediatr. gastroenterol. nutr.

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“…On the pooled stool collection, total fat concentration was determined by the method of Van de Kamer, modified according to Jeejeboy et al (11). Fecal nitrogen content was assayed by the nearinfrared reflectance analysis (NIRA) method (360 InfrAlyzer; Bran + Lubbe Technicon Instruments, Roma, Italy), according to Peuchant et al (12). Fecal energy content was derived from wet stool weight using the regression equation of Murphy et al (13).…”

Section: Methodsmentioning

confidence: 99%

High‐ Versus Low‐Lipase Acid‐Resistant Enzyme Preparations in Cystic Fibrosis: A Crossover Randomized Clinical Trial

Lancellotti,

Cabrini,

Zanolla

et al. 1996

J. pediatr. gastroenterol. nutr.

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SummaryHigh‐strength pancreatic enzyme preparations have recently come into widespread use in some countries for treatment of pancreatic insufficiency in cystic fibrosis. However, the therapeutic equivalence of these preparations to the standard acid‐resistant microsphere preparations, under the same lipase dosage, has not been demonstrated by appropriate clinical trials; they are also considered responsible for severe colonic stricture. In a randomized crossover study, 20 adolescent or adult cystic fibrosis patients were treated in hospital with both low‐lipase (A) and high‐lipase (B) enteric‐coated microsphere preparations. The fat excretion coefficient, evaluated over two 72‐h fat balance periods (measured fat intake, 1.43 to 3 g/kg/day according to age), was the main response variable, secondary variables being stool wet and dry weight, fecal nitrogen output, and energy loss. With both preparations, patients were given a daily dose of 1,500‐2,000 lipase BP U/g fat ingested, distributed across four meals. The low‐strength preparation was divided into three doses during each meal, while the high‐strength preparation was taken as a single dose in the middle of each meal. The considerable variability of results did not provide conclusive evidence of equivalence or significant differences between the two preparations in terms of steatorrhea and other variables. However, mean differences between the two treatments and their 95% confidence intervals showed less satisfactory results with the high‐lipase preparation. A high‐strength preparation is thought to release relatively less enzyme activity in the small intestine, forcing patients to increase their dosage and possibly creating a dangerous enzyme hyperconcentration in the large intestine. For this reason, the occasional occurrence of colonic stricture should be borne in mind, as must the possible scope for division of dosage during each meal.

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“…The measurement of cytochrome-c oxidase using near-IR has been reported (4). Although transcutaneous applications have not yet been demonstrated, near-IR has been used for the quantitation of serum lipids (5) and fats in human samples (6). Given the success of pulse oximetry, it is possible that this technology can be used to monitor lipids, fats, and other analytes.…”

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confidence: 99%

Contributions of Analytical Chemistry to the Clinical Laboratory

Skogerboe¹

1988

Anal. Chem.

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Value of a spectroscopic "fecalogram" in determining the etiology of steatorrhea.

Cited by 28 publications

References 5 publications

Simultaneous Determination of Fecal Fat, Nitrogen, and Water by Near‐Infrared Reflectance Spectroscopy

Simultaneous Determination of Fecal Fat, Nitrogen, and Water by Near‐Infrared Reflectance Spectroscopy

High‐ Versus Low‐Lipase Acid‐Resistant Enzyme Preparations in Cystic Fibrosis: A Crossover Randomized Clinical Trial

Contributions of Analytical Chemistry to the Clinical Laboratory

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