Simultaneous Determination of Fecal Fat, Nitrogen, and Water by Near‐Infrared Reflectance Spectroscopy

Abstract

BackgroundDeterminations of fecal fat and nitrogen reveal evidence of malabsorption and assist in estimating the efficacy of pancreatic enzyme treatment. Seventy‐two‐hour stool collection, with chemical analysis of fecal fat, and Kjeldahl's method for measurement of fecal nitrogen are generally accepted as standard methods for making these determinations. However, these traditional methods are expensive, timeconsuming, and cumbersome. This study evaluated the efficiency and validity of an alternative method, using nearinfrared reflectance spectroscopy (NIRS) and compared results with those of the standard methods.MethodsNear‐infrared reflectance spectroscopy is a secondary method: The instrument first has to be calibrated with samples analyzed by the standard method. Sixty‐three stool samples with known fat content (range 4.79‐292.5 mg/g), 24 samples with known nitrogen content (range 5.36‐19.38 mg/g), and 24 samples with known water concentration (range 60.1‐82.22%) served for calibration. A further 69 samples were analyzed to validate the procedure.ResultsThere was a satisfactory correlation between the measurements produced by near‐infrared reflectance spectroscopy and those produced by standard methods: fat r = 0.97; nitrogen r = 0.94; water r = 0.96.ConclusionsNear‐infrared reflectance spectroscopy appears to be a reliable, simple, and rapid method of measuring different fecal components‐as precise and accurate as the standard methods. Stool samples should be analyzed immediately after collecting or stored only a few days before analyzing.