Valorization of the antioxidant, enzyme inhibition and phytochemical propensities of Berberis calliobotrys Bien. ex Koehne: A multifunctional approach to probe for bioactive natural products

Khan, Shakeel Ahmad; Nazir, Mamona; Saleem, Hammad; Raiz, Naheed; Saleem, Muhammad; Anjum, Syed Muhammad Muneeb; Zengin, Gökhan; Mukhtar, Mahreen; Tousif, Muhammad Imran; Mahomoodally, Fawzi M.; Ahemad, Nafees

doi:10.1016/j.indcrop.2019.111693

Cited by 24 publications

(19 citation statements)

References 29 publications

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“…The increasing popularity to use plant‐derived nutraceuticals and functional foods requires exploring hidden sources rich in phenolics, which substantiate the significance of measuring total bioactive secondary metabolites or phenolic compounds of a plant to authenticate the utilization of even a wild plant in formulating new nutraceuticals and functional foods. The redox properties of phenolic rich herbs and formulations are responsible for antioxidant activity and to maintain human health [14–16] . Antioxidant properties of phenolics are attributed to the presence of at least one hydroxy group on aromatic ring, [17] which acts as an electron donor and thus directly involves in quenching free radicals [18] .…”

Section: Resultsmentioning

confidence: 99%

Investigation on the Phytochemical Composition, Antioxidant and Enzyme Inhibition Potential of Polygonum Plebeium R.Br: A Comprehensive Approach to Disclose New Nutraceutical and Functional Food Ingredients

Saleem

Shazmeen

Nazir

et al. 2021

Chemistry & Biodiversity

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The present work describes medicinal potential and secondary metabolic picture of the methanol extract (PP-M) of Polygonum plebeium R.Br. and its fractions; hexane (PP-H), ethyl acetate (PP-E) and water (PP-W). In total bioactive component estimation, highest contents of phenolic (89.38 � 0.27 mgGAE/g extract) and flavonoid (51.21 � 0.43 mgQE/g extract) were observed in PP-E, and the same fraction exhibited the highest antioxidant potential in DPPH (324.80 � 4.09 mgTE/g extract), ABTS (563.18 � 11.39 mgTE/g extract), CUPRAC (411.33 � 15.49 mgTE/g extract) and FRAC (369.54 � 1.70 mgTE/g extract) assays. In Phosphomolybdenum activity assay, PP-H and PP-E showed nearly similar potential, however, PP-H was the most active (13.54 � 0.24 mgEDTAE/g extract) in metal chelating activity assay. PP-W was the stronger inhibitor (4.03 � 0.05 mgGALAE/g extract) of the enzyme AChE, while PP-H was potent inhibitor of BChE (5.62 � 0.27 mg GALAE/g extract). Interestingly, PP-E was inactive against BChE. Against tyrosinase activity, PP-E was again the most active fraction with inhibitory value of 71.89 � 1.44 mg KAE/g extract, followed by the activity of PP-M and PP-W. Antidiabetic potential was almost equally distributed among PP-M, PP-H and PP-E. For mapping the chemodiversity of P. plebeium, PP-M was analyzed through UHPLC/MS, which led to the identification of more than 50 compounds. Flavonoids were the main components derived from isovitexin, kaempferol and luteolin however, gallic acid, protocatechuic acid, gingerols and lyoniresinol 9'-sulfate were among important bioactive phenols. These findings prompted to conclude that Polygonum plebeium can be a significant source to offer new ingredient for nutraceuticals and functional foods.

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Section: Resultsmentioning

confidence: 99%

Investigation on the Phytochemical Composition, Antioxidant and Enzyme Inhibition Potential of Polygonum Plebeium R.Br: A Comprehensive Approach to Disclose New Nutraceutical and Functional Food Ingredients

Saleem

Shazmeen

Nazir

et al. 2021

Chemistry & Biodiversity

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“…ex Koehne. 21 With an increasing demand for natural antioxidants, more plants that are suitable for development as natural antioxidants need to be identified.…”

Section: Introductionmentioning

confidence: 99%

Terpenoid composition and antioxidant activity of extracts from four chemotypes of Cinnamomum camphora and their main antioxidant agents

Luo

Tian

Zheng

et al. 2021

Biofuels Bioprod Bioref

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Cinnamomum camphora (L.) J. Presl can produce an abundance of terpenoids, and has four main chemotypes: linalool, eucalyptol, camphor, and borneol. Antioxidant activity and the chemical composition of extracts from the four chemotypes were investigated during spring (May), summer (July), and autumn (November) with the aim of developing natural antioxidants using suitable chemotypes of C. camphora in suitable seasons, together with their main terpenoids. The main antioxidant terpenoids were identified. The four chemotype extracts showed strong scavenging activity against 2,2-diphenyl-1picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radicals as well as O 2 -• and •OH in July, due to the fact that their terpenoid content was at its highest during this month. Among the four chemotypes, the strongest scavenging activity was mainly detected in the linalool chemotype extracts, which had the highest terpenoid content. Among the ten main terpenoids in C. camphora extracts, ocimene, linalool, β-pinene, longifolene and eucalyptol showed stronger scavenging activity against DPPH free radicals, and ocimene exhibited the strongest scavenging activity. Extracts from the four chemotypes of C. camphora and their main terpenoids have the potential for development as natural antioxidants, with summer as the best season, linalool chemotypes as the optimum choice, and ocimene as the optimum compound.

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“…Interestingly, our findings showed that both crude and anthocyanin-rich extracts of Calafate fruits were able to reduce in vitro viability and migration of AGS (gastric cancer) and G415 (gallbladder cancer) human cell lines. Several studies have reported that many species of Berberis have cardiovascular and hepatoprotective effects as well as antimicrobial and anti-cancerous activities (Adamus et al 2019;Alamgeer et al 2017;Behravan et al 2019;Boudjlida et al 2019;Khan et al 2019;Srivastava et al 2015). These effects have been explained mainly for their high contents of alkaloids such as berberine, which are the main secondary metabolite in berberis species (Hussain et al 1981) with proved therapeutic effects in many fields of medicine (Srivastava et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, Khan et al (2019) pointed out that, among plant secondary metabolites, phenolic compounds have received considerable attention, being the most studied natural product class for pharmaceutical and dietary concerns owing to their significant antiallergic, antimicrobial, anti-inflammatory, and antioxidant activities. Plant phenolics have radical scavenging and metal chelating potential, being useful to the treatment of worldwide diseases like cardiovascular disorders and cancer (Mocan et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Anthocyanin-Rich Extracts of Calafate (Berberis microphylla G. Forst.) Fruits Decrease In Vitro Viability and Migration of Human Gastric and Gallbladder Cancer Cell Lines

Calderón-Reyes

Pezoa

Leal

et al. 2020

J Soil Sci Plant Nutr

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Currently, gastric cancer (GC) and gallbladder cancer (GBC) constitute important causes of human deaths related to cancer worldwide. In the last years, several researches are focused on the role of dietary compounds in preventing cancers. The consumption of fruits with high antioxidants, mainly anthocyanins, represents a good option to reduce the risk of chronic human diseases. Calafate (Berberis microphylla G. Forst.) berries, recognized by their remarkable antioxidant properties and high content of anthocyanins, appear as a new alternative to treat degenerative diseases of public interest. The present work was aimed to evaluate the impact of crude and anthocyanin-rich extracts from Calafate fruits on in vitro cell viability and migration capacity of gastric (AGC) and gallbladder (G415) human cancer cell lines, as related with their antioxidant properties. Crude and anthocyanin-rich extracts were obtained from fruits of Calafate grown under field conditions in the south of Chile. Antioxidants, phenols, anthocyanins, and anthocyanidins were determined. In vitro cell viability and migration of AGS and G415 human cancer cell lines at different concentrations of extracts (25-800 μg mL −1) were determined. Anthocyanin-rich extracts of Calafate berries showed comparable antioxidant activity (up to 1200 μg Trolox eq. g −1 DW), slightly lower total phenolic content (12%), but higher total anthocyanin content (25%) compared to the crude extract. The major anthocyanidin molecule detected in both extracts was delphinidin, followed by malvidin, and low concentrations of petunidin, cyanidin, and peonidin. As expected, all of these compounds were detected in higher levels in anthocyanin-rich extracts (up to 2-fold). Noteworthy, our study revealed that Calafate fruit extracts strongly decrease in vitro viability and migration capacity of gastric carcinoma (AGC model) and gallbladder carcinoma (G415 model) human cell lines; however, the anthocyanin-rich extract displayed higher inhibitory effects (up to 70%) compared to crude extracts. These findings allow suggesting that the in vitro antiproliferative potential of Calafate fruit extract is strongly related to the anthocyanin concentration, especially delphinidin.

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Valorization of the antioxidant, enzyme inhibition and phytochemical propensities of Berberis calliobotrys Bien. ex Koehne: A multifunctional approach to probe for bioactive natural products

Cited by 24 publications

References 29 publications

Investigation on the Phytochemical Composition, Antioxidant and Enzyme Inhibition Potential of Polygonum Plebeium R.Br: A Comprehensive Approach to Disclose New Nutraceutical and Functional Food Ingredients

Investigation on the Phytochemical Composition, Antioxidant and Enzyme Inhibition Potential of Polygonum Plebeium R.Br: A Comprehensive Approach to Disclose New Nutraceutical and Functional Food Ingredients

Terpenoid composition and antioxidant activity of extracts from four chemotypes of Cinnamomum camphora and their main antioxidant agents

Anthocyanin-Rich Extracts of Calafate (Berberis microphylla G. Forst.) Fruits Decrease In Vitro Viability and Migration of Human Gastric and Gallbladder Cancer Cell Lines

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