2017
DOI: 10.1371/journal.pone.0175130
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Validation of two multiplex platforms to quantify circulating markers of inflammation and endothelial injury in severe infection

Abstract: Biomarkers can prognosticate outcome and enable risk-stratification. In severe infection, focusing on multiple markers reflecting pathophysiological mechanisms of organ injury could enhance management and pathway-directed therapeutics. Limited data exist on the performance of multiplex biomarker platforms. Our goal was to compare endothelial and immune activation biomarkers in severe pediatric infections using two multiplex platforms. Frozen plasma from 410 children presenting to the Jinja Regional Hospital in… Show more

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Cited by 64 publications
(50 citation statements)
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“…The clinical samples were centrifuged at the hospital, and serum was stored at -20°C. Commercial ELISAs were used to measure biomarker levels (ANGPT1, ANGPT2, sICAM1, sTREM1, CHI3L1, PF4, CXCL10, sFLT1, sTEK, VEGF: R&D Systems; vWF: antibody from Dako, standard from American Diagnostica) (38). All assays were conducted in duplicate.…”
Section: Methodsmentioning
confidence: 99%
“…The clinical samples were centrifuged at the hospital, and serum was stored at -20°C. Commercial ELISAs were used to measure biomarker levels (ANGPT1, ANGPT2, sICAM1, sTREM1, CHI3L1, PF4, CXCL10, sFLT1, sTEK, VEGF: R&D Systems; vWF: antibody from Dako, standard from American Diagnostica) (38). All assays were conducted in duplicate.…”
Section: Methodsmentioning
confidence: 99%
“…Magnetic Luminex® Assays was a magnetic bead-based antibody microarray founded upon the sandwich immunoassay principle, which can be used to facilitate the simultaneous quantitation of up to 100 soluble analytes in a single sample [29]. APRIL, MCP-1 and L-selectin concentrations were determined by the Luminex magnetic bead cytokines panel based on the competition principle (R&D Systems, Inc. Minneapolis, MN, USA).…”
Section: Luminex Assaysmentioning
confidence: 99%
“…Eleven of 13 biomarkers of endothelial or immune activation 6− 13 used in our previously reported study in febrile adults 14 were quanti ed using the multiplex Luminex® platform (Luminex, Austin TX) with custom-developed reagents (R&D Systems, Minneapolis, MN) 39 in the 2084 children with available plasma who had been regularly discharged from hospital or had died (see Supplementary Information). sTREM-1 and sFlt-1, which ranked rst and second in the analysis of the derivation cohort, sTNFR1, which ranked second after sTREM-1 in the previously reported study in febrile adults in Tanzania, 14 and Ang-2, which was the biomarker used for sample size considerations of the current study, were subsequently quanti ed using identical methods in the remaining 376 children with available plasma who were transferred or absconded.…”
Section: Quanti Cation Of Biomarkersmentioning
confidence: 99%