Validation of an assay for the determination of cotinine and 3-hydroxycotinine in human saliva using automated solid-phase extraction and liquid chromatography with tandem mass spectrometric detection

Bentley, M.C.; Abrar, Mohammed; Kelk, Mark; Cook, Jeremy J.; Phillips, K.

doi:10.1016/s0378-4347(98)00494-0

Cited by 64 publications

(41 citation statements)

References 15 publications

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“…Determination of nicotine metabolism/pharmacokinetics provides a useful tool for estimating uptake of nicotine and tobacco-related toxicants, for understanding the pharmacologic effects of nicotine and nicotine addiction, and for optimizing nicotine dependency treatment. Metabolites of nicotine, such as cotinine, have been used as biomarkers of nicotine and tobacco smoke exposure (1)(2)(3)(4). Serum nicotine and urinary/saliva cotinine have been used to guide the dose of nicotine replacement therapy (5,6 ).…”

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confidence: 99%

Simultaneous and Sensitive Measurement of Anabasine, Nicotine, and Nicotine Metabolites in Human Urine by Liquid Chromatography–Tandem Mass Spectrometry

2004

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Methods:We developed a sensitive method for analysis of nicotine and five major nicotine metabolites, including cotinine, trans-3-hydroxycotinine, nicotine-N-oxide, cotinine-N-oxide, and nornicotine, in human urine by liquid chromatography coupled with a TSQ Quantum triple quadrupole tandem mass spectrometer (LC/ MS/MS). Urine samples to which deuterium-labeled internal standards had been added were extracted with a simple solid-phase extraction procedure. Anabasine, a minor tobacco alkaloid, was also included. Results: The quantification limits of the method were 0.1-0.2 g/L, except for nicotine (1 g/L). Cotinine-Noxide, trans-3-hydroxycotinine, nicotine, and anabasine in urine were almost completely recovered by the solidphase extraction, whereas the mean extraction recoveries of nicotine-N-oxide, cotinine, and nornicotine were 51.4%, 78.6%, and 78.8%, respectively. This procedure provided a linearity of three to four orders of magnitude for the target analytes: 0.2-400 g/L for nicotine-N-

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confidence: 99%

Simultaneous and Sensitive Measurement of Anabasine, Nicotine, and Nicotine Metabolites in Human Urine by Liquid Chromatography–Tandem Mass Spectrometry

2004

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“…It is now recognized that liquid chromatography (LC)-tandem mass spectrometry (MS-MS) is the methodology of choice for the analysis of these compounds in biological fluids, such as serum/ plasma (6-12), urine (8,(13)(14)(15)(16), saliva (17), teeth (18), and meconium (19). The majority of published methods use atmospheric pressure chemical ionization (APCI) (6,7,9,14,17) or electrospray ionization (ESI) (8,10,11,16).…”

Section: Introductionmentioning

confidence: 99%

“…The majority of published methods use atmospheric pressure chemical ionization (APCI) (6,7,9,14,17) or electrospray ionization (ESI) (8,10,11,16). The method we describe uses dualmode solid phase extraction (SPE) for sample preparation, hydrophilic interaction chromatography (HILIC) for LC separation, and positive ESI and multiple reaction monitoring (MRM) for mass spectrometric detection to simultaneously determine nicotine and the four related analytes in serum, plasma, or urine.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS

Yue

Kushnir

Urry

et al. 2009

Methods in Molecular Biology

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We describe a method for the quantitative analysis of nicotine, cotinine, trans-3'-hydroxy cotinine, nornicotine, and anabasine in urine, serum, and plasma using liquid chromatography-tandem mass spectrometry. A mix of deuterium-labeled internal standards (IS) is added to a specimen aliquot. The aliquot is extracted using mixed-mode solid phase extraction and eluted into an autosampler vial for injection into an LC-MS-MS system. An Atlantis silica column is used for LC separation in hydrophilic interaction mode. Tandem mass spectrometry detection is performed in positive ion mode with electrospray ionization and two multiple reaction monitoring (MRM) transitions monitored for each analyte and IS.

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“…Several methods were published including radioimmunoassay [6], enzyme-linked immunoassay [7], gas chromatography (GC) [8,9] or gas chromatography coupled to mass spectrometry (GC/MS) [10][11][12][13][14][15], high-performance liquid chromatography (HPLC) [16][17][18], or LC/MS/MS [19][20][21]. These techniques generally require liquid-liquid extraction (LLE) or solid phase extraction (SPE) for biological specimen clean-up and concentration prior to chromatography.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of nicotine, cotinine, norcotinine, and trans-3′-hydroxycotinine in human oral fluid using solid phase extraction and gas chromatography–mass spectrometry

Kim

Darwin

Huestis

2005

Journal of Chromatography B

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Validation of an assay for the determination of cotinine and 3-hydroxycotinine in human saliva using automated solid-phase extraction and liquid chromatography with tandem mass spectrometric detection

Cited by 64 publications

References 15 publications

Simultaneous and Sensitive Measurement of Anabasine, Nicotine, and Nicotine Metabolites in Human Urine by Liquid Chromatography–Tandem Mass Spectrometry

Simultaneous and Sensitive Measurement of Anabasine, Nicotine, and Nicotine Metabolites in Human Urine by Liquid Chromatography–Tandem Mass Spectrometry

Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS

Simultaneous determination of nicotine, cotinine, norcotinine, and trans-3′-hydroxycotinine in human oral fluid using solid phase extraction and gas chromatography–mass spectrometry

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