Simultaneous and Sensitive Measurement of Anabasine, Nicotine, and Nicotine Metabolites in Human Urine by Liquid Chromatography–Tandem Mass Spectrometry

Abstract

Methods:We developed a sensitive method for analysis of nicotine and five major nicotine metabolites, including cotinine, trans-3-hydroxycotinine, nicotine-N-oxide, cotinine-N-oxide, and nornicotine, in human urine by liquid chromatography coupled with a TSQ Quantum triple quadrupole tandem mass spectrometer (LC/ MS/MS). Urine samples to which deuterium-labeled internal standards had been added were extracted with a simple solid-phase extraction procedure. Anabasine, a minor tobacco alkaloid, was also included. Results: The quantification limits of the method were 0.1-0.2 g/L, except for nicotine (1 g/L). Cotinine-Noxide, trans-3-hydroxycotinine, nicotine, and anabasine in urine were almost completely recovered by the solidphase extraction, whereas the mean extraction recoveries of nicotine-N-oxide, cotinine, and nornicotine were 51.4%, 78.6%, and 78.8%, respectively. This procedure provided a linearity of three to four orders of magnitude for the target analytes: 0.2-400 g/L for nicotine-N-

High-Throughput Simultaneous Analysis of Five Urinary Metabolites of Areca Nut and Tobacco Alkaloids by Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry with On-Line Solid-Phase Extraction

“…Tuomi et al (29) and Xu et al (30) described LC-MS/MS methods involving an off-line (manual) SPE cleanup for nicotine and cotinine and had detection limits of 1 to 10 ng/mL and 0.1 to 1 ng/mL, respectively. Similarly, the method described by Heavner et al (31) involved also an off-line SPE and had detection limits of 4.4 and 3.7 ng/mL for nicotine and cotinine, respectively.…”

Measurement of cotinine in urine by liquid chromatography tandem mass spectrometry

“…12,16,18 The measurement of cotinine is not an urgent investigation. In our centre, we run the cotinine assay weekly to assess the smoking status of patients who are being considered for cardiothoracic transplantation while attending the assessment clinics.…”

The Chemical Components of Electronic Cigarette Cartridges and Refill Fluids: Review of Analytical Methods

“…A number of reports [35][36][37][38][39][40][41] on determination of nicotine and related alkaloids by LC-MS/MS in different matrices (especially in biological fluids, like urine, plasma, saliva) have been published. However, these methods can be also suitable for e-liquids and aerosol from ECs, but their applicability to these matrices has to be verified.…”