Validation of a reference measurement procedure for the assessment of selenomethionine in nutritional supplements

Sannac, Sébastien; Pannier, Florence; Oster, Caroline; Labarraque, Guillaume; Fisicaro, Paola; Potin‐Gautier, Martine

doi:10.1039/b808663j

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…Beyond any doubt, procedures based on the hyphenation of gas or liquid chromatography with species-specic or unspecic isotope dilutioninductively coupled plasma mass spectrometryprovide accurate and precise quantication results; [14][15][16][17][18][19] however, they are still too demanding to be implemented in routine analysis. Simplication and miniaturization have been approached in several studies with an emphasis mainly on the sample pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Determination of SeMet and Se(iv) in biofortified yeast by ion-pair reversed phase liquid chromatography-hydride generation-microwave induced nitrogen plasma atomic emission spectrometry (HPLC-HG-MP-AES)

Barrientos

Wróbel

Guzmán

et al. 2016

J. Anal. At. Spectrom.

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Selenium biofortified yeast is the most common dietary Se supplement in human nutrition and in farm animals. Therefore, the production and routine quality control of commercial products are highly demanded. In this work, a simple and cost-effective procedure is proposed for the determination of SeMet and Se(IV) in hydrolyzed yeast, consisting of ion-pair reversed phase separation, post-column hydride generation and Se quantification by atomic emission spectrometry with microwave plasma sustained by nitrogen (HPLC-HG-MP-AES). Freeze-dried biomass was hydrolyzed with methanesulfonic acid; chromatographic separation was performed with a mobile phase containing 0.08% v/v heptafluorobutyric and methanol (92 : 8) at a flow rate of 1 mL min À1 ; the column effluent was on-line mixed with an alkaline solution of potassium persulfate (K 2 S 2 O 8 6% m/v, NaOH 3% m/v), passed through a reaction coil submerged in a water bath at 60 C, and then 10 M hydrochloric acid was added prior to hydride generation in the MP-AES multimode sample introduction system (NaBH 4 2% m/v, NaOH 0.3% m/v). The total chromatographic run was accomplished in 5 min and the evaluated on-column quantification limits were 59 ng Se mL À1 for Se(IV) and 0.52 mg mL À1 for SeMet. The procedure was tested using standardized Seleno Excell® high selenium yeast and then applied for the analysis of Saccharomyces cerevisiae biofortified under different fermentation and exposure conditions. The procedure was capable of detecting differences in selenium concentration among cultures and the results were consistent with those obtained while coupling HPLC separation directly to ICP-MS detection.

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Section: Introductionmentioning

confidence: 99%

Determination of SeMet and Se(iv) in biofortified yeast by ion-pair reversed phase liquid chromatography-hydride generation-microwave induced nitrogen plasma atomic emission spectrometry (HPLC-HG-MP-AES)

Barrientos

Wróbel

Guzmán

et al. 2016

J. Anal. At. Spectrom.

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show abstract

“…Finally, Sannac and co-workers evaluated the uncertainty budgets in the determination of selenomethionine in nutritional supplements 109 and methylmercury in fish products. 110 In both studies it was observed that the factors linked to the measurement, i.e.…”

Section: Uncertainty Propagation Studiesmentioning

confidence: 99%

Recent advances in isotope dilution analysis for elemental speciation

Rodríguez-González

Alonso

2010

J. Anal. At. Spectrom.

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“…A metrological protocol, developed in order to enhance traceability in speciation analysis, the need for which has been demonstrated in inter-laboratory speciation studies, was applied to the determination of SeMet and tSe in two sample types, SELM-1 yeast CRM (NRCC) and yeast based pharmaceutical tablets used in an interlaboratory evaluation exercise, CCQM-P86. 141 Two extraction protocols, enzymatic hydrolysis and a sequential extraction followed by pooling the extracts, were evaluated for their capability to leach out tSe and SeMet from the samples. Speciation analysis was performed by IC-ICP-MS. Isotopically enriched SeMet, with 76 Se, was used for ssIDMS in extracts as well as to determine the tSe contained in the supernatants.…”

Section: Metrology and Crmsmentioning

confidence: 99%