Validated HPLC Method for the Determination of Gliquidone in Rat Plasma

Sridevi, S.; Diwan, Prakash V.

doi:10.1211/146080800128736097

Cited by 9 publications

(6 citation statements)

References 5 publications

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“…Gliquidone is official in BP (Brithish Pharmacopeia, 2016). Literature survey revealed that quantitation of gliquidone has been achieved by UV spectrophotometry (Arayne, Sultana, Mirza, 2006), LC-MS (Maurer et al, 2002) and HPLC in dosage form and biological fluids (Arayne et al, 2010a;Arayne et al, 2010b;Guo et al, 1992;Sridevi, Diwan, 2000). Recently, spectrofluoremetric normal and derivative synchronous methods have been published for the determination of gliquidone in drug substance and drug products (El-Ghobashy et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Forced degradation of gliquidone and development of validated stability-indicating HPLC and TLC methods

Elghobashy

Yehia

Helmy

et al. 2018

Braz. J. Pharm. Sci.

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Forced degradation studies of gliquidone were conducted under different stress conditions. Three degradates were observed upon using HPLC and TLC and elucidated by LC-MS and IR. HPLC method was performed on C 18 column using methanol-water (85:15 v/v) pH 3.5 as a mobile phase with isocratic mode at 1 mL.min-1 and detection at 225 nm. HPLC analysis was applied in range of 0.5-20 µg.mL-1 (r =1) with limit of detection (LOD) 0.177 µg.mL-1. TLC method was based on the separation of gliquidone from degradation products on silica gel TLC F 254 plates using chloroform-cyclohexaneglacial acetic acid (6:3:1v/v) as a developing system with relative retardation 1.15±0.01. Densitometric measurements were achieved in range of 2-20 µg /band at 254 nm (r = 0.9999) with LOD of 0.26 µg /band. Least squares regression analysis was applied to provide mathematical estimates of the degree of linearity. The analysis revealed a linear calibration for HPLC where a binomial relationship for TLC. Stability testing and methods validation have been evaluated according to International Conference on Harmonization guidelines. Moreover, the proposed methods were applied for the analysis of tablets and the results obtained were statistically compared with those of pharmacopeial method revealing no significant difference about accuracy and precision.

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Section: Introductionmentioning

confidence: 99%

Forced degradation of gliquidone and development of validated stability-indicating HPLC and TLC methods

Elghobashy

Yehia

Helmy

et al. 2018

Braz. J. Pharm. Sci.

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“…8 A literature survey revealed that the quantification of gliquidone is achieved by ultraviolet (UV) spectrophotometry, 9 as well as high-performance liquid chromatography (HPLC). 10,11 Our research group earlier reported methods for the simultaneous determination of rosiglitazone and glimepiride by HPLC, and now this method has been developed and used for interaction studies, 12 while enalapril and statins have been used as commercial tablets and human serums. 13 Earlier we reported on the interaction of losartan with gliquidone, as well as pioglitazone and enalapril with H 2 receptor antagonist in combined pharmaceutical dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of in vitro interaction studies of enalapril with hypoglycemic agents by LC-UV

Sultana¹,

Sattar²,

Arayne³

2013

RRMC

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Abstract:The coadministration of antihypertensive and antidiabetic drugs is common, as both of these ailments are synergistic to each other and often occur together. In the present paper, we describe in vitro drug interactions of enalapril, an antihypertensive drug, with the hypoglycemic agents, metformin, glibenclamide, and glimepiride. These studies were carried out using an isocratic reversed phase high-performance liquid chromatographic method using a C18 column with ultraviolet detection at 230 nm. The system was operated at room temperature using a mobile phase consisting of methanol:water (70:30) adjusted to pH 2.5 with o-phosphoric acid with a flow rate of 1 mL minute −1. The assay was reproducible, linear (concentration range of 2.5-100 µg mL ) with a correlation coefficient of 0.9999 and an accuracy rate of 98%-102%. The results from the reversed phase high-performance liquid chromatographic method clearly indicated that the availability of enalapril was unaffected by the simultaneous administration of hypoglycemic agents. Hence, the two drugs can be safely administered with one another.

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“…Literature survey revealed that quantification of gliquidone has been achieved by UV spectrophotometry (11), atmospheric pressure chemical ionization liquid chromatographic-mass spectrometry (APCI-LC-MS), LC-MS (12), and high-performance liquid chromatography (HPLC) (13)(14)(15). Numerous workers have reported determination of H 1 -receptor antagonists by different techniques.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Gliquidone, Fexofenadine, Buclizine, and Levocetirizine in Dosage Formulation and Human Serum by RP-HPLC

Arayne

Sultana

Mirza

et al. 2010

Journal of Chromatographic Science

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In the present paper, a simultaneous method has been developed and validated for estimation of gliquidone in the presence of H(1)-receptor antagonists (fexofenadine hydrochloride, buclizine hydrochloride, and levocetirizine dihydrochloride) using reversed-phase high-performance liquid chromatographic technique. A good chromatographic separation between these drugs was achieved using a mobile phase containing methanol-water (80:20 v/v) at pH 3.5 with a flow rate of 1.0 mL/min; and detection was performed at 230 nm with a UV detector. Validation of the method was performed in terms of linearity, accuracy, precision, and limit of detection and quantification. The linearity of the calibration curves for gliquidone, fexofenadine hydrochloride, buclizine hydrochloride, and levocetirizine dihydrochloride were found to be 0.338-50 microg/mL (r = 0.9964), 5-50 microg/mL (r = 0.9956), 0.325-50 microg/mL (r = 0.9967), and 0.553-50 microg/mL (r = 0.9950), respectively. There was no significant difference between the amount of drug spiked in serum and the amount recovered, and serum did not interfere in simultaneous estimation. Thus, the proposed method is suitable for the simultaneous analysis of active ingredients in tablet dosage forms and human serum.

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Validated HPLC Method for the Determination of Gliquidone in Rat Plasma

Cited by 9 publications

References 5 publications

Forced degradation of gliquidone and development of validated stability-indicating HPLC and TLC methods

Forced degradation of gliquidone and development of validated stability-indicating HPLC and TLC methods

Monitoring of in vitro interaction studies of enalapril with hypoglycemic agents by LC-UV

Simultaneous Determination of Gliquidone, Fexofenadine, Buclizine, and Levocetirizine in Dosage Formulation and Human Serum by RP-HPLC

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