Vagococcus martis sp. nov., isolated from the small intestine of a marten, Martes flavigula

Abstract

A novel coccus-shaped, Gram-stain-positive, non-motile and facultative aerobic bacterium, designated strain D7T301T, was isolated from the small intestine of a marten, Martes flavigula, which was killed on the road in Pocheon-si, Gyeonggi-do, Republic of Korea. Grown on a tryptic soy yeast agar plate, colonies had a creamy colour and irregular form. The new isolate formed a monophyletic clade with Vagococcus penaei CD276T on a phylogenetic consensus tree based on the 16S rRNA gene sequence. The isolate grew optimally at 37 °C and pH 7 in the presence of 0.5 % (w/v) NaCl. The isolate was catalase- and oxidase-negative. The cell-wall peptidoglycan was type A4α l-Lys-d-Asp. The major cellular fatty acids were C16 : 0, C14 : 0, and C16 : 1ω9c. The predominant respiratory quinone was menaquinone MK-7 (85.1 %). The DNA G+C content based on genome sequencing was 33.8 mol%. The average nucleotide identity value obtained from comparative genomic analysis between strain D7T301T and V. penaei CIP 109914T was 72.6 %. On the basis of the phenotypic, phylogenetic, biochemical, chemotaxonomic, and genotypic analyses, Vagococcusmartis is proposed as a novel species of the genus Vagococcus. The type strain is D7T301T (=KCTC 21069T=JCM 31178T).

Lactobacillus hegangensis sp. nov., Lactobacillus suibinensis sp. nov., Lactobacillus daqingensis sp. nov., Lactobacillus yichunensis sp. nov., Lactobacillus mulanensis sp. nov., Lactobacillus achengensis sp. nov., Lactobacillus wuchangensis sp. nov., Lactobacillus gannanensis sp. nov., Lactobacillus binensis sp. nov. and Lactobacillus angrenensis sp. nov., isolated from Chinese traditional pickle and yogurt

“…Tolerance to tellurite and pyruvate utilization were determined as described by Miyashita et al [33]. The cell-wall peptidoglycan amino acids were extracted according to the method of Hasegawa et al and developed as described by Tak et al [34, 35]. Distinctive features among strains 73-4 T , 247-3 T , 143-4(a) T , 33-1 T , 143-6 T , 247-4 T , 17-4 T , 143-1 T , 735-2 T , M1530-1 T and phylogenetically related species are shown in Tables 6–10, and more features are presented in the species description and Table S10.…”

Proposal of Lactobacillus kosoi Chiou et al. 2018 as a later heterotypic synonym of Lactobacillus micheneri McFrederick et al. 2018, elevation of Lactobacillus plantarum subsp. argentoratensis to the species level as Lactobacillus argentoratensis sp. nov., and Lactobacillus zhaodongensis sp. nov., isolated from traditional Chinese pickle and the intestinal tract of a honey bee (Apis mellifera)

“…Tolerance to tellurite and pyruvate utilization were determined as described by Miyashita et al [9]. The cell-wall peptidoglycan amino acids were extracted according to the method of Hasegawa et al and developed as described by Tak et al [56,57]. Distinctive features between strain 1206-1 T and type strains of phylogenetically related species are shown in Table S5.…”

Vagococcus xieshaowenii sp. nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content

“…Since its division from the genus Lactococcus in 1989 [1], the genus Vagococcus has expanded to include 14 validly published species, including Vagococcus fluvialis [1–3], Vagococcus salmoninarum [4], Vagococcus lutrae [5], Vagococcus fessus [6], Vagococcus carniphilus [7], Vagococcus elongatus [8], Vagococcus penaei [9], Vagococcus acidifermentans [10], Vagococcus entomophilus [11], Vagococcus humatus [12], Vagococcus martis [13], Vagococcus teuberi [14], Vagococcus bubulae and Vagococcus vulneris [15]. The cell characteristics of members in the genus Vagococcus are: Gram-stain-positive; coccus-shaped; and C 16 : 0 and C 18 : 1 ω9 c as the major cellular fatty acids.…”

“…MK-7 was the predominant quinone (68.8 %) in strain CF-49 T , lower than the level in V. martis (85.1 %), the only established species in the genus with available information about its respiratory quinone composition [13].…”