Lactobacillus hegangensis sp. nov., Lactobacillus suibinensis sp. nov., Lactobacillus daqingensis sp. nov., Lactobacillus yichunensis sp. nov., Lactobacillus mulanensis sp. nov., Lactobacillus achengensis sp. nov., Lactobacillus wuchangensis sp. nov., Lactobacillus gannanensis sp. nov., Lactobacillus binensis sp. nov. and Lactobacillus angrenensis sp. nov., isolated from Chinese traditional pickle and yogurt

Abstract

Fourteen Gram-stain-positive bacterial strains were isolated from Chinese traditional pickle and yogurt. The strains were characterized using a polyphasic taxonomic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, fatty acid methyl ester analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), in silico DNA-DNA hybridization (isDDH) and an analysis of phenotypic features. The data demonstrated that the 14 strains represented ten novel species belonging to the genus Lactobacillus, strains 73-4 T , 247-3 T , 143-4(a) T , 33-1 T , 143-6 T , 247-4 T , 17-4 T , 143-1 T , 735-2 T and M1530-1 T were designated as the type strains. Strains 73-4 T and 247-3 T were phylogenetically related to the type strains of Lactobacillus camelliae and Lactobacillus jixianensis, having 97.0-98.9 % 16S rRNA gene sequence similarities, 83.9-87.2 % pheS gene sequence similarities and 86.8-93.3 % rpoA gene sequence similarities. Strains 143-4(a) T and 33-1 T were phylogenetically related to the type strains of Lactobacillus rhamnosus, Lactobacillus paracasei and Lactobacillus casei, having 93.6-96.5 % 16S rRNA gene sequence similarities, 73.9-77.2 % pheS gene sequence similarities and 76.1-77.6 % rpoA gene sequence similarities. Strains 143-6 T , 247-4 T , 17-4 T and 143-1 T were phylogenetically related to the type strains of Lactobacillus concavus, Lactobacillus dextrinicus and Lactobacillus bayanensis, exhibiting 95.5-99.9 % 16S rRNA gene sequence similarities, 76.5-83.1 % pheS gene sequence similarities and 83.6-98.3 % rpoA gene sequence similarities. Strain 735-2 T was phylogenetically related to the type strains of Lactobacillus zhaoyuanensis, Lactobacillus jiayinensis and Lactobacillus coryniformis, having 98.2-99.1 % 16S rRNA gene sequence similarities, 82.8-84.1 % pheS gene sequence similarities and 93.0-93.9 % rpoA gene sequence similarities. Strain M1530-1 T was phylogenetically related to the type strains of Lactobacillus suantsaiihabitans and Lactobacillus brevis, having 99.5 and 99.0 % 16S rRNA gene sequence similarities, 90.3 and 81.7 % pheS gene sequence similarities and 97.7 and 91.1 % rpoA gene sequence similarities. The ANI and isDDH values between strains 73-4 T , 247-3 T , 143-4(a) T , 33-1 T , 143-6 T , 247-4 T , 17-4 T , 143-1 T , 735-2 T , M1530-1 T and type strains of phylogenetically related species were less than 86.8 % and 33.9 % respectively, confirming that they represent ten novel species within the genus Lactobacillus. Based upon the data of polyphasic characterization obtained in the present study, ten novel species,

Lacticaseibacillus absianus sp. nov., isolated from the cecum of a mini-pig

“…Strain YH-lac23 T produced two types of lactic acid: d-(3 %) and l-lactate (97 %). This is similar to strain L. porcinae KCTC 21027 T [35]; however, L. daqingensis JCM 33273 T [34] and L. manihotivorans KCTC 21010 T [36] can only produce l-lactate. The dominant whole-cell fatty acids (mean values >10 % of whole cellular fatty acids) of the isolate were C 18 : 1 ω9c (55.8 %) and C 16 : 0 (26.2 %).…”

“…The dominant whole-cell fatty acids (mean values >10 % of whole cellular fatty acids) of the isolate were C 18 : 1 ω9c (55.8 %) and C 16 : 0 (26.2 %). The same dominant cellular fatty acids were also detected in L. daqingensis JCM 33273 T , although at different percentages [34]. The closely related strains L. porcinae KCTC 21027 T and L. manihotivorans KCTC 21010 T also contained summed feature 7 as the major cellular fatty acid, different from the isolate.…”

“…API 50CHL tests revealed that the isolate was positive for amygdalin, lactose and starch, but negative for d -ribose, which is different from the reference strain L. daqingensis JCM 33273 T [34]. The isolate was positive for d -fructose, d -tagatose methyl α- d -glucoside, melibiose, raffinose, glycogen and turanose, which is different from the reference strain L.…”

A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae

“…L. achengensis grows between 10 and 37°C but not at 5 and 45°C and metabolises several pentoses and a broad spectrum of disaccharides [338]. The genome size of the type strain is 2.64 Mbp.…”

New combinations, synonymy and emendations can only be proposed based on names that were previously validly published

“…The names of the following 11 new combinations were derived from basonyms that were not validly published at the time the paper was submitted for publication. The corresponding basonyms were published in articles in the same issue of the IJSEM on pages preceding those of the Zheng et al article [3, 4]. The following comb.…”