Vacuolar Acid Hydrolysis as a Physiological Mechanism for Sucrose Breakdown

Echeverrı́a, Ed; Burns, J. C.

doi:10.1104/pp.90.2.530

Cited by 50 publications

(39 citation statements)

References 12 publications

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“…2; 2013 (Echeverria & Burns, 1989). It was also possible that acids were used for alcoholic fermentation in harvested citrus (Echeverria & Burns, 1989). The results showed that the decreases in TA and citric acid content were faster during storage on-tree than at 25°C.…”

Section: Changes In Ta Juice Ph and Organic Acids Contentsmentioning

confidence: 59%

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Influence of Storage Conditions on Physico-Chemical and Biochemical of Two Tangerine Cultivars

Roongruangsri

Rattanapanone²,

Leksawasdi³

et al. 2013

JAS

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The influences of on-tree, off-tree and cold storage on physico-chemical and biochemical were studied and quite similar between thetwo tangerine cultivars, 'Sai Num Phueng' and 'See Thong'. In both cultivars, the percentage of weight loss increased and the moisture content of the peel (%) decreased at higher temperature and longer duration. Low temperature storage at 5°C reduced the losses of fruit weight and moisture content of the peel and preserved the external quality better than 25°C storage. During on-tree and off-tree storage at 25 and 5°C, peel chlorophyll content decreased and peel carotenoid content increased. Titratable acidity (TA), citric acid, isocitric acid and vitamin C contents decreased and juice pH increased. Malic acid content stayed relatively constant. Total soluble solids (TSS), TSS/TA ratio and sucrose content continually increased. Glucose and fructose contents slightly increased. The peel chlorophyll content, TA and citric acid content decreased faster during storage on-tree than at 25°C. The peel carotenoid, TSS and sucrose contents increased greater during storage on-tree than at 25°C. The delayed declining of peel chlorophyll, TA, and citric acid contents and the slow increases of the peel carotenoid, TSS and sucrose contents were observed at 5°C storage in comparison to 25°C. The activities of mitochondrial citrate synthase, cytosolic aconitase and cytosolic NADP-isocitrate dehydrogenase (NADP-IDH) were lower and decreased faster during storage at 25°C in comparison to storage on-tree. The activities of these enzymes, which related to citric acid metabolism, were slowed down which resulted in delaying the decrease of citric acid content during storage at 5°C in comparison to 25°C.

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Section: Changes In Ta Juice Ph and Organic Acids Contentsmentioning

confidence: 59%

“…5, No. 2; 2013 (Echeverria & Burns, 1989). It was also possible that acids were used for alcoholic fermentation in harvested citrus (Echeverria & Burns, 1989).…”

Section: Changes In Ta Juice Ph and Organic Acids Contentsmentioning

confidence: 99%

Influence of Storage Conditions on Physico-Chemical and Biochemical of Two Tangerine Cultivars

Roongruangsri

Rattanapanone²,

Leksawasdi³

et al. 2013

JAS

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“…Second, even if sucrose were hydrolyzed and rapidly resynthesized during transport, percentages of "'C-hexoses indicate that the process occurs to approximately the same degree throughout the vascular, nonvascular, and sink tissues examined. In addition, activities of acid invertases in these tissues drop to barely detectable levels at this stage of development (23), and pH along the transport path is not likely to be low enough for rapid action of the nonenzymatic sucrose hydrolysis reported in acid limes (5). The suggestion that sucrose hydrolysis in this system is more closely related to storage than transport is also supported by the observation that instances of most pronounced formation of "'C-hexoses occurred in the final storage sites (juice sacs) rather than en route.…”

Section: Sucrose Hydrolysismentioning

confidence: 99%

Postphloem, Nonvascular Transfer in Citrus

Koch¹,

Avigne²

1990

Plant Physiol.

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“…Vacuoles also function as a depository of xenobiotic and toxic compounds, minimizing toxicity in the cytoplasm where sensitive biochemical processes take place. Secondary metabolites and proteins involved in plant defense against pathogens and herbivores are also kept in the vacuole and released in response to attack and cellular damage (5). Vacuoles play a critical role in pH regulation as well, with its own pH typically around 5-6 because of action of H + -ATPases and pyrophosphatase that pump protons into the lumen (6).…”

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confidence: 99%

Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis

Zhang

Zhao

Tang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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The central vacuole in a plant cell occupies the majority of the cellular volume and plays a key role in turgor regulation. The vacuolar membrane (tonoplast) contains a large number of transporters that mediate fluxes of solutes and water, thereby adjusting cell turgor in response to developmental and environmental signals. We report that two tonoplast Detoxification efflux carrier (DTX)/Multidrug and Toxic Compound Extrusion (MATE) transporters, DTX33 and DTX35, function as chloride channels essential for turgor regulation in Arabidopsis. Ectopic expression of each transporter in Nicotiana benthamiana mesophyll cells elicited a large voltage-dependent inward chloride current across the tonoplast, showing that DTX33 and DTX35 each constitute a functional channel. Both channels are highly expressed in Arabidopsis tissues, including root hairs and guard cells that experience rapid turgor changes during root-hair elongation and stomatal movements. Disruption of these two genes, either in single or double mutants, resulted in shorter root hairs and smaller stomatal aperture, with double mutants showing more severe defects, suggesting that these two channels function additively to facilitate anion influx into the vacuole during cell expansion. In addition, dtx35 single mutant showed lower fertility as a result of a defect in pollen-tube growth. Indeed, patch-clamp recording of isolated vacuoles indicated that the inward chloride channel activity across the tonoplast was impaired in the double mutant. Because MATE proteins are widely known transporters of organic compounds, finding MATE members as chloride channels expands the functional definition of this large family of transporters.vacuole | root hair | stomata | drought

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Vacuolar Acid Hydrolysis as a Physiological Mechanism for Sucrose Breakdown

Cited by 50 publications

References 12 publications

Influence of Storage Conditions on Physico-Chemical and Biochemical of Two Tangerine Cultivars

Influence of Storage Conditions on Physico-Chemical and Biochemical of Two Tangerine Cultivars

Postphloem, Nonvascular Transfer in Citrus

Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis

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