Vaccination Against Red Sea Bream Iridoviral Disease in Red Sea Bream.

Nakajima, Kazuhiro; Maeno, Yukio; Kurita, Jun; Inui, Yasuo

doi:10.3147/jsfp.32.205

Cited by 73 publications

(40 citation statements)

References 7 publications

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“…On the other hand, PCR products were not obtained from the 10 surviving fish that were examined from the vaccinated group. The formalininactivated RSIV vaccine has been shown previously to confer high levels of protection against RSIV disease in red sea bream Pagrus major (Nakajima et al 1997), yellowtail Seriola quinqueradiata, amberjack S. dumerili, kelp grouper Epinephelus moara, striped jack Pseudocaranx dentex and spotted parrot fish Oplegnathus punctatus under laboratory conditions . Moreover, it has been shown that vaccination of fish with an RSIV vaccine triggers a specific immune response as indicated by an enhanced expression of major histocompatibility complex (MHC) class I and increased levels of serum neutralization antibodies (Caipang et al 2006).…”

Section: Resultsmentioning

confidence: 99%

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Histopathological and ultrastructural features of enlarged cells of humpback grouper Cromileptes altivelis challenged with Megalocytivirus (family Iridoviridae) after vaccination

Mahardika¹,

Haryanti²,

Muzaki‬³

et al. 2008

Dis. Aquat. Org.

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The genus Megalocytivirus in the family Iridoviridae encompasses isolates of red sea bream iridovirus (RSIV) and grouper sleepy disease iridovirus (GSDIV). In the present study, humpback grouper Cromileptes altivelis juveniles were challenged with GSDIV after vaccination with a commercial RSIV vaccine. The unvaccinated group (in duplicate) showed higher mortalities (59.3 to 66.7%) than the vaccination group (0% mortalitiy, in duplicate). Surviving fish in the vaccinated group displayed masses of enlarged cells in the spleen. Electron microscopy revealed that they contained hemosiderin granules within the cytoplasm. In contrast, moribund fish from the unvaccinated group exhibited large numbers of inclusion body-bearing cells (IBCs) in the spleen, while surviving fish displayed masses of enlarged cells in which a small number of GSDIV virions were assembled. KEY WORDS: Humpback grouper · Cromileptes altivelis · Commercial RSIV vaccine · Grouper sleepy disease iridovirus · Enlarged cells Resale or republication not permitted without written consent of the publisherDis Aquat Org 79: [163][164][165][166][167][168] 2008 Chua et , Matsuoka et al. 1996, Danayadol et al. 1997, Kasornchandra & Khongpradit 1997, Chou et al. 1998, Jung & Oh 2000, Chao et al. 2002, Sano et al. 2002, Sudthongkong et al. 2002b, Weng et al. 2002, Chen et al. 2003, GibsonKueh et al. 2003, Shi et al. 2004. A diagnostic feature of megalocytivirus disease is the formation of inclusion body-bearing cells (IBCs) as well as necrotized cells, both of which are observed as enlarged cells by light microscopy (Miyazaki 2007).Recently, a commercialized formalin-inactivated RSIV vaccine has been shown to be highly efficient in protecting vaccinated red sea bream Pagrus major and other cultured marine fishes against RSIV disease (RSIVD) (Nakajima et al. 1997. However, the ultrastructural features of fish vaccinated against megalocytivirus were not investigated.In order to compare histopathological changes in unvaccinated, surviving fish after challenge with GSDIV with changes in surviving fish after being vaccinated and challenged with GSDIV, we undertook vaccination trials using a commercialized formalininactivated RSIV vaccine 'Biken' (The Research Foundation for Microbial Diseases of Osaka University, Kagawa, Japan). The present study describes the results of the vaccination test and ultrastructural features of the enlarged cells that developed in surviving fish of the vaccinated and unvaccinated groups. MATERIALS AND METHODSExperimental fish. For vaccination experiments, a total of 120 humpback grouper (mean body weight, 5 g) were obtained from a fish breeder in Bali Island. Ten samples of these were examined by PCR assay using a primer set for SJNNV (striped jack nervous necrosis virus) (Nishizawa et al. 1994) and for Pst I restriction fragment of RSIV genomic DNA (Kurita et al. 1998), and all were negative for the presence of NNV (nervous necrosis virus: Betanodavirus) and megalocytivirus.Virus stock. The GSDIV stock used in the experiment...

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Section: Resultsmentioning

confidence: 99%

“…Recently, a commercialized formalin-inactivated RSIV vaccine has been shown to be highly efficient in protecting vaccinated red sea bream Pagrus major and other cultured marine fishes against RSIV disease (RSIVD) (Nakajima et al 1997. However, the ultrastructural features of fish vaccinated against megalocytivirus were not investigated.…”

Section: Introductionmentioning

confidence: 99%

Histopathological and ultrastructural features of enlarged cells of humpback grouper Cromileptes altivelis challenged with Megalocytivirus (family Iridoviridae) after vaccination

Mahardika¹,

Haryanti²,

Muzaki‬³

et al. 2008

Dis. Aquat. Org.

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“…It presents the highest identity to those of LCDV-1 and other iridoviruses among the putative gene products of LCDV-C. Homology analysis showed that the MCPs of LCDV-1 (33), CIV (16), TFV (14), FV3 (20), EHNV (22), BIV (3), RSIV (23), and ISKNV (13) had 87.6, 53.0, 51.1, 50.9, 50.7, 50.7, 49.0, and 49.2% identity to that of LCDV-C, respectively. Based on the multiple alignments of amino acid sequences of nine complete MCPs, a phylogenetic tree was constructed.…”

Section: Vol 78 2004mentioning

confidence: 99%

Complete Genome Sequence of Lymphocystis Disease Virus Isolated from China

Zhang

Xiao

Xie

et al. 2004

J Virol

125

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Lymphocystis diseases in fish throughout the world have been extensively described. Here we report the complete genome sequence of lymphocystis disease virus isolated in China (LCDV-C), an LCDV isolated from cultured flounder (Paralichthys olivaceus) with lymphocystis disease in China. The LCDV-C genome is 186,250 bp, with a base composition of 27.25% G؉C. Computer-assisted analysis revealed 240 potential open reading frames (ORFs) and 176 nonoverlapping putative viral genes, which encode polypeptides ranging from 40 to 1,193 amino acids. The percent coding density is 67%, and the average length of each ORF is 702 bp. A search of the GenBank database using the 176 individual putative genes revealed 103 homologues to the corresponding ORFs of LCDV-1 and 73 potential genes that were not found in LCDV-1 and other iridoviruses. Among the 73 genes, there are 8 genes that contain conserved domains of cellular genes and 65 novel genes that do not show any significant homology with the sequences in public databases. Although a certain extent of similarity between putative gene products of LCDV-C and corresponding proteins of LCDV-1 was revealed, no colinearity was detected when their ORF arrangements and coding strategies were compared to each other, suggesting that a high degree of genetic rearrangements between them has occurred. And a large number of tandem and overlapping repeated sequences were observed in the LCDV-C genome. The deduced amino acid sequence of the major capsid protein (MCP) presents the highest identity to those of LCDV-1 and other iridoviruses among the LCDV-C gene products. Furthermore, a phylogenetic tree was constructed based on the multiple alignments of nine MCP amino acid sequences. Interestingly, LCDV-C and LCDV-1 were clustered together, but their amino acid identity is much less than that in other clusters. The unexpected levels of divergence between their genomes in size, gene organization, and gene product identity suggest that LCDV-C and LCDV-1 shouldn't belong to a same species and that LCDV-C should be considered a species different from LCDV-1.

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“…However, control measures for this disease have not been established. Recently, we reported on the effectiveness of a vaccine against RSIVD under laboratory conditions (Nakajima et al 1997). Herein, w e describe the results regarding the effectiveness of the vaccine evaluated in a field trial.…”

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confidence: 99%