UV Spectrophotometric Determination of Hydrochlorothiazide and Olmesartan Medoxomil in Pharmaceutical Formulation

Abstract

A simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of hydrochlorothiazide and olmesartan medoxomil in combined dosage form by UV spectrophotometric method. UV spectrophotometric method includes simultaneous equation method (Method I) 271.5 nm and 257.0 nm λ max of both the drugs were selected, absorbance Ratio method (Method II) 261.5 nm an isoabsorptive wavelength and 257.0 nm were selected for estimation of hydrochlorothiazide and olmesartan medoxomil respectively and Three wavelength method (Method III), two wavelengths were selected such that hydrochlorothiazide give same absorbances (263.8 and 278.4 nm) at two selected wavelength while third wavelength (316.5 nm) was such that olmesartan gives nearly zero absorbance. The two drugs follow Beer's law over the concentration range of 5-25 µg/mL. The % recoveries of the both the drugs were found to be nearly 100 % representing the accuracy of the proposed methods. Validation of the proposed methods was carried out for its accuracy, precision, specificity and ruggedness according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of hydrochlorothiazide and olmesartan medoximil in combined dosage form.

Low-Viscosity Hydroxypropylcellulose (HPC) Grades SL and SSL: Versatile Pharmaceutical Polymers for Dissolution Enhancement, Controlled Release, and Pharmaceutical Processing

“…These samples were then transferred to Costar clear 96-well plates and analyzed using a UV detector (SpectraMax M2, Molecular Devices, Pennsylvania, USA). Drug concentrations in the dissolution samples were determined based upon linear concentration-absorbance plots generated at 285, 250, 271, and 265 nm for CBZ, PHT, HCTZ, and CPM, respectively (24)(25)(26)(27).…”

Development of Spectrophotometric Method for Quantitative Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

“…Amlodipine besylate is official in IP [2] and BP [3] and Hydrochlorthiazide is official in IP [2], BP [3] and USP [4]. Literature survey revealed that various methods such as UV [9,10,15,16,19,20], HPLC [11,17], HPTLC [12,18], LC-MS/MS [13] and UPLC [14] are available in single and combination with other drugs. However, no spectrophotometric method has yet been reported for simultaneous estimation of amlodipine besylate, olmesartan medoxomil and hydrochlorthiazide in tablet dosage forms.…”

Definition of System Suitability Test Limits on the Basis of Robustness Test Results

“…The numerous analytical methods dealt with assay of HCT, OLM, PRL, and AMD available [15][16][17][18][19][20] are impaired by inefficient or timeconsuming procedures and lack of statistical evaluation of significant variables. No HPLC method has been developed for simultaneous analysis of the four drugs so far.…”