Development of Spectrophotometric Method for Quantitative Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

Sharma, Hemendra Kumar; Jain, Nayan

doi:10.4172/2153-2435.1000126

Cited by 14 publications

(7 citation statements)

References 9 publications

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“…• A simple, rapid and precise reversed-phase liquid chromatographic method is developed for simultaneous determination of Atorvastatin, Ezetimibe and Fenofibrate in their ternary mixture of commercial pharmaceutical preparations. This HPLC method is successfully applied to the simultaneous quantitative analysis of the drugs in tablets [42].…”

Section: Applications Of This Methods In Clinical Samplesmentioning

confidence: 99%

Recent Trends in Analytical Techniques for the Development of Pharmaceutical Drugs

Ravali¹

2011

JBABM

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Growing interest in the evolution of Pharmaceutical Drugs had made to bring certain analytical techniques into limelight. Many such techniques are used to extract, purify and characterize (structurally and molecularly), newly produced vital drugs. This review gives information regarding emerging trends in using various analytical techniques like HPLC, HPTLC, LC-MS/MS, etc.In development of newly producing drugs. The proposed methods were statistically equivalent and showed satisfactory results.

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Section: Applications Of This Methods In Clinical Samplesmentioning

confidence: 99%

Recent Trends in Analytical Techniques for the Development of Pharmaceutical Drugs

Ravali¹

2011

JBABM

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“…Few methods are available for the simultaneous analysis of OLM and AML combination. These methods include spectrophotometry [35][36][37][38][39][40][41][42], HPLC [36,43,44] and TLC [44]. These methods suffered from lower sensitivity and selectivity (e.g.…”

Section: Introductionmentioning

confidence: 99%

Multivariate Versus Classical Univariate Calibration Methods for Spectrofluorimetric Data: Application to Simultaneous Determination of Olmesartan Medoxamil and Amlodipine Besylate in their Combined Dosage Form

Darwish

Backeit

2012

J Fluoresc

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Olmesartan medoxamil (OLM, an angiotensin II receptor blocker) and amlodipine besylate (AML, a dihydropyridine calcium channel blocker), are co-formulated in a single-dose combination for the treatment of hypertensive patients whose blood pressure is not adequately controlled on either component monotherapy. In this work, four multivariate and two univariate calibration methods were applied for simultaneous spectrofluorimetric determination of OLM and AML in their combined pharmaceutical tablets in all ratios approved by FDA. The four multivariate methods are partial least squares (PLS), genetic algorithm PLS (GA-PLS), principal component ANN (PC-ANN) and GA-ANN. The two proposed univariate calibration methods are, direct spectrofluorimetric method for OLM and isoabsorpitive method for determination of total concentration of OLM and AML and hence AML by subtraction. The results showed the superiority of multivariate calibration methods over univariate ones for the analysis of the binary mixture. The optimum assay conditions were established and the proposed multivariate calibration methods were successfully applied for the assay of the two drugs in validation set and combined pharmaceutical tablets with excellent recoveries. No interference was observed from common pharmaceutical additives. The results were favorably compared with those obtained by a reference spectrophotometric method.

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“…HCZ is official in IP 2 and USP 3 . Analytical methods that have been reported for the estimation of HCZ in bulk drug and their formulation include UV spectrophotometric and HPLC as single or in combination of AML 50,51 OLM [58][59][60][61][62][63] , nebivolol hydrochloride 64,66 , Eprosartan 66 , Telmisartan 67 and UV method has been reported for estimation of all three drugs 68 . However, no RP-HPLC method has yet been reported for simultaneous estimation of AML, OLM and HCZ in tablet dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Rp-HPLC Method for Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

Jain¹

2014

Int. J. Res. Ayurveda Pharm.

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A new, simple, accurate, precise and reproducible reverse phase high performance liquid chromatography method was developed and fully validated for the simultaneous estimation of amlodipine besylate, olmesartan medoxamil and hydrochlorothiazide in tablet dosage form isocratically using acetonitrile: methanol: phosphate buffer pH-3.0 (48:12:40 % v/v/v) as mobile phase and Prontosil C-18 column (4.6 x 250 mm, 5 μ particle size) as stationary phase and chromatogram was recorded at 232 nm at a flow rate of 1.2 mL/min. The calibration curves obtained were linear (r 2 = 0.9998) over the concentration range of 5-25 µg/ml, 5-25 µg/ml and 5-25 µg/ml for amlodipine besylate, olmesartan medoxamil and hydrochlorothiazide respectively. A run time of 7.0 minutes for each sample made it possible to analyze more than 200 samples per day. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the method can be used for routine monitoring of amlodipine besylate, olmesartan medoxamil and hydrochlorothiazide in industry in the assay of bulk drug and dosage form.

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Development of Spectrophotometric Method for Quantitative Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

Cited by 14 publications

References 9 publications

Recent Trends in Analytical Techniques for the Development of Pharmaceutical Drugs

Recent Trends in Analytical Techniques for the Development of Pharmaceutical Drugs

Multivariate Versus Classical Univariate Calibration Methods for Spectrofluorimetric Data: Application to Simultaneous Determination of Olmesartan Medoxamil and Amlodipine Besylate in their Combined Dosage Form

Development and Validation of Rp-HPLC Method for Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

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