UV-Irradiated DNA Matrixes Selectively Bind Endocrine Disruptors with a Planar Structure

Abstract

DNA has unique chemical properties and is readily purified from salmon milts and shellfish gonads. DNA has few commercial uses and is generally discarded as an industrial waste. Recently, we prepared water-insoluble and nuclease-resistant DNA-films by UV irradiation. The DNA-films removed DNA-intercalating compounds. Here, we immobilized double-stranded DNA onto porous glass beads by UV irradiation and prepared DNA-immobilized glass bead columns. The DNA-immobilized columns effectively accumulated more DNA-intercalating materials than the DNA-films. The DNA-immobilized columns bound endocrine disruptors with a planar structure, such as dioxin-derivatives, polychlorobiphenyl (PCB)-derivatives, and benzo[a]pyrene. Bisphenol A and diethylstilbestrol, which lack a planar structure, did not bind to the DNA-immobilized columns. These results suggest that DNA-immobilized glass bead columns could selectively remove chemical compounds with a planar structure by intercalation into the double-stranded DNA. DNA-immobilized glass beads and DNA-films prepared by UV irradiation have the potential to serve as useful biomaterials for medical, engineering, and environmental applications.

DNA-Modified Polysulphone Microspheres for Endocrine Disruptors and Heavy Metal Ions Removal

“…This explains why the value of the octanol-water distribution coefficient had such an important effect on the removal ratio (Zhao et al 2004c). In addition, it has been reported that double-stranded DNA has a bias towards the selective removal and accumulation of endocrine disruptors with a planar structure (Yamada et al 2002). Both BP and DBF have planar structures, whereas that of DBD is not planar.…”

“…As a result, the utilization of DNA as a functional material is limited. Water-insoluble DNA film can be prepared by UV irradiation (Yamada et al 2002) while insolubilized DNA may be immobilized onto porous glass beads (Yamada et al 2000). Both DNA films and DNA-immobilized glass beads are capable of removing compounds which are intercalated into DNA, such as endocrine disruptors and heavy metal ions.…”

“…Several studies have reported the removal of endocrine disruptors via different methods (Yamada et al 2000(Yamada et al , 2002Yoon et al 2003;Kang and Kondo 2002). Some kinds of heavy metal ions can also be regarded as endocrine disruptors.…”

“…Some kinds of heavy metal ions can also be regarded as endocrine disruptors. A wide variety of adsorbents has been reported in the literature for heavy metal ion adsorption (Yamada et al 2000(Yamada et al , 2002Bassi et al 2000;Denizli et al 2002;Arpa et al 2002).…”

Bio-Inspired Membranes for Advanced Polymer Electrolyte Fuel Cells. Anhydrous Proton-Conducting Membrane via Molecular Self-Assembly

“…[33][34][35] Bio-inspired materials or natural biopolymers have attracted much attention for a industrial applications as a biocompatible material. [36][37][38][39][40][41][42][43][44][45][46] Although these materials are often discarded as industrial waste around the world, there is an increasing interest towards the application of the biomaterials in devices because they have well-controlled structures and superior conducting properties. The controlled hydrogen-bonded network in biomolecules, such as bioenergetic proteins, is essential for efficient energy transduction in living systems, 47,48 and these biomolecules may be efficient electrolytes with superior conducting properties.…”