Preparation and Characterization of DNA Films Induced by UV Irradiation

Abstract

Large amounts of DNA‐enriched materials, such as salmon milts and shellfish gonads, are discarded as industrial waste. We have been able to convert the discarded DNA to a useful material by preparing novel DNA films by UV irradiation. When DNA films were irradiated with UV light, the molecular weight of DNA was greatly increased. The reaction was inhibited by addition of the radical scavenger galvinoxyl suggesting that the DNA polymerization with UV irradiation proceeded by a radical reaction. Although this UV‐irradiated DNA film was water‐insoluble and resistant to hydrolysis by nuclease, the structure of the DNA film in water was similar to non‐irradiated DNA and maintained B‐form structure. In addition, the UV‐irradiated DNA film could effectively accumulate and condense harmful DNA‐intercalating compounds, such as ethidium bromide and acridine orange, from diluted aqueous solutions. The binding constant and exclusion number of ethidium bromide for UV‐irradiated DNA were determined to be 6.8±0.3×104 M−1 and 1.6±0.2, respectively; these values are consisted with reported results for non‐irradiated DNA. The UV‐irradiated DNA films have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds.

Drug Binding and Releasing Characteristics of DNA/Lipid/PLGA Film

“…In recent years, strategies to apply deoxyribonucleic acid (DNA) as a biomaterial in tissue engineering have been developed and carried out [1][2][3][4][5] . Such immense interest in DNA has arisen because of the latter' s favorable properties as a biomaterial [6][7][8] .…”

“…However, native DNA alone is limited in its use as a carrier because of its water-soluble property 10) . To overcome this limitation, several techniques of forming water-insoluble DNA complexes have been reported 4,11,12) . Amongst which, Tanaka and Okahata 11) developed a technique to form DNA/cationic lipid complexes.…”

Bio-Inspired Membranes for Advanced Polymer Electrolyte Fuel Cells. Anhydrous Proton-Conducting Membrane via Molecular Self-Assembly

“…[33][34][35] Bio-inspired materials or natural biopolymers have attracted much attention for a industrial applications as a biocompatible material. [36][37][38][39][40][41][42][43][44][45][46] Although these materials are often discarded as industrial waste around the world, there is an increasing interest towards the application of the biomaterials in devices because they have well-controlled structures and superior conducting properties. The controlled hydrogen-bonded network in biomolecules, such as bioenergetic proteins, is essential for efficient energy transduction in living systems, 47,48 and these biomolecules may be efficient electrolytes with superior conducting properties.…”

Accumulation-Exclusion Combined System for the DNA-Binding Harmful Chemicals with Insolubilized DNA

“…The DNA microarray technique 3 is a good example using such special function of DNA. DNA-columns, DNA-gels, DNA-films, and a variety of DNA-containing composites [4][5][6][7][8][9][10][11][12][13][14][15][16][17] have been prepared by covalent linking [4][5][6][7][8][9][10][11][12][13] or conjugation with other polymers. [14][15][16][17] DNA materials have shown their potential utility as chemosensory devices for DNA binding species.…”

“…6 The formation mechanism was estimated to be based on intermolecular multipoint cross-linking induced by radical formation. This method possesses some advantages.…”