Utilization of Rice Husks and Groundnut Shells for Bioethanol Production

Rabah, Abdullahi Bako; Oyeleke, Solomon Bankole; Manga, S. B.; Gusau, Lawal Hassan

doi:10.7763/ijesd.2014.v5.511

Cited by 7 publications

(8 citation statements)

References 4 publications

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“…Saccharomyces cerevisiae are seen to be smooth creamish, white spherical shape, Grampositive, non-motile, catalase positive, the organisms was able to ferment glucose, fructose, sucrose, maltose and galactose, producing acid and gas. The result is in agreement with that of Rabah et al [19] who isolated Saccharomyces cerevisiae from palm wine [17] who isolated Saccharomyces cerevisiae from palm wine juice in sake-type fermentation, Moneke et al [30] reported the isolation of Saccharomyces cerevisiae from orchard soil and Tambuwal et al [29] reported the isolation of Saccharomyces cerevisiae from Hibiscus sadriffa (Zobo).…”

Section: Saccharomyces Cerevisiae Isolated From Locally Fermeneted Beverage ("Kunun-zaki") Samplessupporting

confidence: 89%

“…Samples of rotten oranges and locally fermented beverage (kunu-zaki) were collected from minor refuse dumps at the Muda Lawan orange market of Bauchi metropolis for the isolation of Zymomonas mobilis and Saccharomyces cerevisiae. The rotten orange samples were placed in clean sterile polythene bags and locally fermented beverage collected in sterile container and transported immediately to the laboratory for analysis, as described by reference [19].…”

Section: Collection and Processing Of Samplesmentioning

confidence: 99%

“…The amounts of sugar in the hydrolysed samples were determined by the use of the spectrophotometer at the wavelength of 491nm as described by Rabah et al [19]. Whereby 2cm 3 of the samples collected were each treated with 2cm 3 of DNS solution and heated in a water bath for 15 minutes to develop the reddish brown colouration and thus, equalled with 1cm 3 of 40% rochellet salt solution and allowed to cooled; then, measured at 491nm using the spectrophotometer.…”

Section: Determination Of Reducing Sugarmentioning

confidence: 99%

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Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Na’Allah

Iliyasu

Haruna

et al. 2021

MRJI

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Background of Study: Plant waste such as rice husk and groundnut shell are generated in large amounts, these waste presents a tremendous pollution to the environment. Worldwide, these wastes are often simply dumped into landfills and oceans or used as animal feeds. The recovery of food processing wastes as renewable energy sources represents a sustainable option for the substitution of fossil energy in order to minimize environmental damages and to meet energy demands of the growing population. Aim: To produce bioethanol from rice husk and groundnut shell using local strains of Zymomonas mobilis and Saccharomyces cerevisiae. Place and Duration of Study: Conducted at the Microbiology Laboratory of Abubakar Tafawa Balewa University Bauchi, Bauchi state, Nigeria, between April to June, 2021. Methods: Groundnut shell and Rice husk were collected from local milling center. The wastes were powdered, sieved and used as carbon source. Proximate composition of the subsrate was done and the total carbohydrate was determined by difference. The sum of the percentage moisture, ash, crude lipid, crude protein and crude fibre was subtracted from 100. Zymomonas mobilis and Saccharomyces cerevisiae were isolated from rotten sweet oranges and locally fermented beverage (‘kunun-zaki’) respectively by growing them on Malt Yeast Peptone Glucose Agar (MYPGA) after which they were further screened for their ability to tolerate ethanol and they serve as organisms for fermentation. The enzyme α- amylase was used for hydrolysis. The fermented substrates were distilled at 78oC and the distillate was collected as bioethanol in a conical flask. UV-VIS spectrophotometer was used to determine the absorbance of each concentration (0, 0.2, 0.4, 0.6 and 0.8cm3) of reducing sugar content of the hydrolysates and the bioethanol produced by developing a standard curve at a wavelength of 491nm and 588nm respectively. The concentration of reducing sugar and bioethanol was determined using a reference line from the Standard curve. Results: Proximate analysis done shows that rice husk have 70.09% carbohydrates while groundnut shell has 65.09% carbohydrates. Groundnut shell yielded the highest reducing sugar of 5.096%. Rice husk yielded the lowest quantity of reducing sugar with a total yield of 2.962%. Maximum concentration of bioethanol of 0.971% was produced from the combination of Saccharomyces cerevisiae and Zymomonas mobilis from groundnut shell. The lowest concentration of 0.121% of bioethanol was produced when Saccharomyces cerevisiae was used on rice husk hydrolysates. The synergistic relationship of Saccharomyces cerevisiae and Zymomonas mobilis yielded the maximum bioethanol when compared with the yield obtained when the organisms were used singly. Zymomonas mobilis produced highest bioethanol content when the organisms are used single. Conclusion: This study demonstrates the potentiality of local strains of Saccharomyces cerevisiae and Zymomonas mobilis isolated from rotten sweet orange and locally fermented beverage (‘kunun-zaki’) to produce bioethanol by fermenting the rice husk and groundnut shell hydrolysates.

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Section: Saccharomyces Cerevisiae Isolated From Locally Fermeneted Beverage ("Kunun-zaki") Samplessupporting

confidence: 89%

Section: Collection and Processing Of Samplesmentioning

confidence: 99%

Section: Determination Of Reducing Sugarmentioning

confidence: 99%

See 1 more Smart Citation

Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Na’Allah

Iliyasu

Haruna

et al. 2021

MRJI

View full text Add to dashboard Cite

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“…Then 1 ml of each biofuel samples were put into test tubes and treated with 2 mL of the chromium reagent. The mixture was allowed to stand for an hour and the absorbance measured at 588 nm using the Ultraviolet-Visible spectrophotometer [14].…”

Section: Determination Of Biofuel Qualitymentioning

confidence: 99%

Biohydrolysis of Banana and Plantain Peels for the Production of Biofuel

Jumare

Magashi

Rabah

et al. 2019

JENRR

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This study was carried out to assess the potentials of banana and plantain peel as feedstock for biofuel production. Fungi were isolated from spoiled banana, burkutu and spoiled bread using the standard microbiological method. The concentration of reducing sugar of the peels were measured using DNS calorimetry method and biofuel were measured using chromium (VI) reagent by Ultraviolet-Visible Spectrophotometer. Aspergillus niger, Saccharomyces cerevisiae and Mucor racemusus were isolated. A reducing sugar concentration of 59.12 mg/g and 56.62 mg/g was observed for the banana and plantain peels. The highest concentration was found to be 0.35 mg/L for banana peels and 0.10 mg/L for plantain. The IR characterization of the banana and plantain sample revealed an intense strong broad band of alcohol O-H and alkane C-H stretching. The GC-MS result revealed the presence of benzaldehyde in all the biomass while 2,3-butanediol was only detected in the plantain peels biomass. This study showed the potential of banana and plantain peels biomass for biofuel production.

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“…Ethanol is an alternative fuel derived from renewable biological resources. It's a good substitute for gasoline in spark ignition engines [3].…”

Section: Introductionmentioning

confidence: 99%

Production of Bioethanol from Sugarcane Bagasse Using Saccharomyces Cerevisiae

Gani

Abdulkadir

Usman

et al. 2018

BJI

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Ethanol is an alternative fuel derived from renewable biological resources. It's a good substitute for gasoline in spark ignition engines. In this study, the sugar cane bagasse was chemically pretreated with 1% NaOH at room temperature for 2 hours. Dilute acid H 2 SO 4 and Aspergillus niger was used to hydrolyse the biomass to sucrose. Fermentation of the hydrolysed sample was done using Saccharomyces cerevisiae. The fermented product was purified by distillation process at 78 o C, and the fraction was collected, and the ethanol was determined by measuring the specific gravity. The production of ethanol from sugar cane bagasse with Saccharomyces cerevisiae was determined after the inoculation into sample A 1 , A 2 and B 1 and B 2 and highest ethanol produced were from B 1 with 0.090 followed B 2 0.074, A 2 with 0.069% and D 0.116. The use of Saccharomyces cerevisiae gives a better yield. The result of this study can be of a better application in the large production of biofuel from sugar cane bagasse which is renewable and highly abundant, it is saving costs by Original Research Article

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Utilization of Rice Husks and Groundnut Shells for Bioethanol Production

Cited by 7 publications

References 4 publications

Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates

Biohydrolysis of Banana and Plantain Peels for the Production of Biofuel

Production of Bioethanol from Sugarcane Bagasse Using Saccharomyces Cerevisiae

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