Production of Bioethanol from Sugarcane Bagasse Using Saccharomyces Cerevisiae

Abstract

Ethanol is an alternative fuel derived from renewable biological resources. It's a good substitute for gasoline in spark ignition engines. In this study, the sugar cane bagasse was chemically pretreated with 1% NaOH at room temperature for 2 hours. Dilute acid H 2 SO 4 and Aspergillus niger was used to hydrolyse the biomass to sucrose. Fermentation of the hydrolysed sample was done using Saccharomyces cerevisiae. The fermented product was purified by distillation process at 78 o C, and the fraction was collected, and the ethanol was determined by measuring the specific gravity. The production of ethanol from sugar cane bagasse with Saccharomyces cerevisiae was determined after the inoculation into sample A 1 , A 2 and B 1 and B 2 and highest ethanol produced were from B 1 with 0.090 followed B 2 0.074, A 2 with 0.069% and D 0.116. The use of Saccharomyces cerevisiae gives a better yield. The result of this study can be of a better application in the large production of biofuel from sugar cane bagasse which is renewable and highly abundant, it is saving costs by Original Research Article

Degradation of lignocellulosic substrates by Pleurotus ostreatus and Lentinus squarrosulus

“…[37]; Shankarappa, et al [38]; Gani, et al [39]. Gani, et al [39] reported high reducing sugar when sugarcane bagasse was pretreated with alkaline and acid.…”

“…[37]; Shankarappa, et al [38]; Gani, et al [39]. Gani, et al [39] reported high reducing sugar when sugarcane bagasse was pretreated with alkaline and acid. The ability of Pleurotus ostreatus and Lentinus squarrosulus to produce lignocellulolytic enzymes that can breakdown cellulose, hemicellulose, and lignin to simple sugar could be responsible for high amount of reducing sugar recorded in degraded rice straw Jonathan and Akinfemi [36]; Belal [40]; Nurika, et al [41].…”

Pembuatan Bioetanol Dari Tebu

“…Maka pengolahan tebu menjadi bioetanol (C2H5OH) sebagai bahan bakar alternatif sangat berguna dan membantu untuk mengatasi kekurangan bahan bakar yang semakin menipis, ditambah kebutuhan akan bahan bakar semakin meningkat dikarenakan jumlah transportasi yang terus meningkat baik dari transportasi umum maupun pribadi sehingga perlu diteliti bahan bakar yang dapat diperbaharui untuk menggantikan bahan bakar dari minyak bumi [4]. Untuk itu dilakukan penelitian dari tebu menjadi bahan bakar bioetanol (C2H5OH) yang sangat penting untuk diteliti guna mendapatkan bahan bakar terbarukan yang dapat membantu mengatasi permasalahan dari minyak bumi [5].…”

Potential Utilization of Sugarcane Bagasse as Raw Material for Bioethanol Production

“…Sugarcane bagasse, which becomes the excess burden in many sugar cane mills in Indonesia has the potential to be a renewable resource for alternative energy that being environmental friendly to substitute crude oil [1], through the production of second generation of bioethanol [2]. Bioethanol stems from ethanol compound that obtained from biomass engineers (plant) that contains sugar, glucose, and cellulose through the biological process utilizing microbial activity [3], [4].…”