Utilization of coulometric array detection in analysis of beverages and plant extracts

Beňová, Blanka; Hájek, Tomáš

doi:10.1016/j.proche.2009.12.015

Cited by 9 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In comparison with other reports that employed HPLC-ECD [ 35 , 47 , 48 ] for phenolic acid detection, LOD observed in this work for p-coumaric acid was lower. Such trend was also verified when CoulArray was employed for polyphenols detection [ 47 , 51 ] with LODs estimated in the present study for vanillic and caffeic acids being similar or lower than previously reported. Use of PAD for cleaning and reactivation of GCE surface probably promoted such increase of sensitivity in relation to other methods, mainly when phenolic acids with high predisposition to adsorption and polymerization, as vanillic and p-coumaric acids, on the electrode surface.…”

Section: Resultssupporting

confidence: 91%

Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Freitas

Silva

Beluomini

et al. 2018

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L−1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coefficients, above 0.995. Linear ranges of the analytes, in mg L−1, were of 0.018–18 (gallic acid), 0.146–19 (vanillic acid), 0.13–17 (caffeic acid), 0.016–16 (ferulic acid), and 0.008–17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 μg·L−1 and precision varied in 1.73–3.78% interval. Concentrations of 19 ± 0.51 mg·L−1 and 7.8 ± 2.5 mg·L−1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.

show abstract

Section: Resultssupporting

confidence: 91%

Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Freitas

Silva

Beluomini

et al. 2018

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

show abstract

“…This is the first report about the quantification of the total content of monomers and dimers of proanthocyanidins in rhizomes of Japanese, Bohemian and giant knotweed. Other authors reported only the contents of separate monomers ((+)-catechin and (−)-epicatechin) [ 7 , 8 , 23 ] and B-type dimers (specified as “procyanidin dimer B”) [ 7 , 8 ] in the rhizomes of Japanese [ 7 , 8 , 23 ] and giant [ 7 , 8 ] knotweed. The total contents of monomers and dimers expressed per dry mass of the plant material (DM) for all STSs were the highest in Japanese knotweed rhizomes (5.80 kg/t DM) and the lowest in Bohemian knotweed rhizomes (2.61 kg/t DM), while it was 4.53 kg/t DM in gi ant knotweed rhizomes ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Flavan-3-ols and Proanthocyanidins in Japanese, Bohemian and Giant Knotweed

Bensa

Glavnik

Vovk

2021

Plants

View full text Add to dashboard Cite

Flavan-3-ols and proanthocyanidins of invasive alien plants Japanese knotweed (Fallopia japonica Houtt.), giant knotweed (Fallopia sachalinensis F. Schmidt) and Bohemian knotweed (Fallopia × bohemica (Chrtek & Chrtkova) J.P. Bailey) were investigated using high performance thin-layer chromatography (HPTLC) coupled to densitometry, image analysis and mass spectrometry (HPTLC–MS/MS). (+)-Catechin, (−)-epicatechin, (−)-epicatechin gallate and procyanidin B2 were found in rhizomes of these three species, and for the first time in Bohemian knotweed. (−)-Epicatechin gallate, procyanidin B1, procyanidin B2 and procyanidin C1 were found in giant knotweed rhizomes for the first time. Rhizomes of Bohemian and giant knotweed have the same chemical profiles of proanthocyanidins with respect to the degree of polymerization and with respect to gallates. Japanese and Bohemian knotweed have equal chromatographic fingerprint profiles with the additional peak not present in giant knotweed. Within the individual species giant knotweed rhizomes and leaves have the most similar fingerprints, while the fingerprints of Japanese and Bohemian knotweed rhizomes have additional peaks not found in leaves. Rhizomes of all three species proved to be a rich source of proanthocyanidins, with the highest content in Japanese and the lowest in Bohemian knotweed (based on the total peak areas). The contents of monomers in Japanese, Bohemian and giant knotweed rhizomes were 2.99 kg/t of dry mass (DM), 1.52 kg/t DM, 2.36 kg/t DM, respectively, while the contents of dimers were 2.81 kg/t DM, 1.09 kg/t DM, 2.17 kg/t DM, respectively. All B-type proanthocyanidins from monomers to decamers (monomers—flavan-3-ols, dimers, trimers, tetramers, pentamers, hexamers, heptamers, octamers, nonamers and decamers) and some of their gallates (monomer gallates, dimer gallates, dimer digallates, trimer gallates, tetramer gallates, pentamer gallates and hexamer gallates) were identified in rhizomes of Bohemian knotweed and giant knotweed. Pentamer gallates, hexamers, hexamer gallates, nonamers and decamers were identified for the first time in this study in Bohemian and giant knotweed rhizomes.

show abstract

“…When the obtained LODs using amperometric detection were compared with previously published data, again the obtained values in this work were better or very similar to those reported (Zima et al ., ; Gao et al ., ). In the coulometric detection mode, our LOD values also are lower than those reported in previous studies (Jandera et al ., ; Kahoun et al ., ; Benova and Hájek, ). The very good LODs obtained for the three detection modes used in this work should be noted, especially using the coulometric detection.…”

Section: Resultsmentioning

confidence: 99%

“…Methods based on HPLC also have been developed for the determination of these compounds. Reverse‐phase (RP) HPLC usually has been employed for the analysis of complex mixtures of phenolic compounds (Schwarz et al ., ; Viñas et al ., ; Larcher et al ., ; Villa et al ., ; Zima et al ., ; Ali et al ., ; Kahoun et al ., ; Muñoz‐Muñoz et al ., ; Benova and Hájek, ; Gao et al ., ; Turek and Stintzing, ; Haddad et al ., ). Although the UV detector is the more common mode (Villa et al ., ; Ali et al ., ; Muñoz‐Muñoz et al ., ; Haddad et al ., ) other detection techniques, such as fluorimetry (Viñas et al ., ) and UV spectrophotometry combined with MS (Turek and Stintzing, ), also have been used.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, using this detection method it is possible to achieve high sensitivity, especially in the coulometric mode. Because of these interesting advantages, electrochemical detection has been used for the determination of phenolic compounds in plants, herbal products, beverages and food (Schwarz et al ., ; Jandera et al ., ; Larcher et al ., ; Zima et al ., ; Kahoun et al ., ; Benova and Hájek, ; Gao et al ., ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitive and Selective Determination of Phenolic Compounds from Aromatic Plants Using an Electrochemical Detection Coupled with HPLC Method

et al. 2014

View full text Add to dashboard Cite

show abstract

Utilization of coulometric array detection in analysis of beverages and plant extracts

Cited by 9 publications

References 15 publications

Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Flavan-3-ols and Proanthocyanidins in Japanese, Bohemian and Giant Knotweed

Sensitive and Selective Determination of Phenolic Compounds from Aromatic Plants Using an Electrochemical Detection Coupled with HPLC Method

Contact Info

Product

Resources

About