Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry

Abstract

A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L−1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coefficients, above 0.995. Linear ranges of the analytes, in mg L−1, were of 0.018–18 (gallic acid), 0.146–19 (vanillic acid), 0.13–17 (caffeic acid), 0.016–16 (ferulic acid), and 0.008–17 (p-coumaric acid), respectively. Limits of detection ranged from 1.6 to 97 μg·L−1 and precision varied in 1.73–3.78% interval. Concentrations of 19 ± 0.51 mg·L−1 and 7.8 ± 2.5 mg·L−1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample. Gallic, ferulic, and p-coumaric acids were not detected. Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.

Evaluation of Opuntia ficus-indica potential as a natural coadjuvant for vinasse treatment

“…Phenol is a recalcitrant compound (Othman et al 2008) and the melanoidins are considered the most recalcitrant dye (Johnson et al 2019). Likewise, phenolic and polyphenolic compounds in vinasses can inhibit microorganisms (Freitas et al 2018;Ao et al 2020).…”

Molecularly Imprinted Polymers-Coated CdTe Quantum Dots for Highly Sensitive and Selective Fluorescent Determination of Ferulic Acid

“…Accordingly, the developed method was sufficiently practical for selective quantification of FA. To further investigate the developed performance, the results with other methods were compared [5–7, 9–11, 24]. As shown in Table 1, different methods have different superiorities and disadvantages.…”

“…It is therefore necessary to quantify FA [4]. For the sensitive determination of FA, several methods have been developed including high-performance liquid chromatography (HPLC) [5, 6], electrochemical techniques [7, 8], Raman spectroscopy [9], chemiluminescence [10], and fluorescence [11]. However, some of these methods need professional operators and/or sophisticated instruments.…”

Wastewater as a resource for pest control: An overview

“…The vinasse, a liquid residue from alcoholic fermentation, contains various compounds such as alcohols, aldehydes, phenols, and acids (Couallier et al 2006;Freitas et al 2018;Fuess et al 2018). In some cases, these compounds are undesirable.…”