Utilização de três métodos imuno-histoquímicos na detecção de aspergilose e zigomicose em animais

Galiza, Glauco José Nogueira de; Tochetto, Camila; Rosa, Fernanda A.; Panziera, Welden; Silva, Taiara M. da; Caprioli, Rafaela Albuquerque; Kommers, Gláucia D.

doi:10.1590/s0100-736x2014000700005

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…Nesse estudo, a PCR demonstrou elevada sensibilidade (80%) e especificidade (100%) na detecção do DNA desse agente micótico em amostras de lesões teciduais, proporcionando uma ferramenta rápida para o diagnóstico de C. lamprauges. O diagnóstico definitivo também pode ser obtido pela identificação de antígenos de C. lamprauges pela IHQ, técnica que se mostrou muito útil na detecção e confirmação dos casos de zigomicose nas espécies ovina, bovina, suína e canina (Galiza et al, 2014), fato atribuído à sua capacidade de fornecer um diagnóstico rápido e preciso, em comparação aos cultivos micológicos, que normalmente requerem maior tempo e experiência do micologista (Ubiali et al, 2013). Desta forma, os exames IHQ, PCR ou ambos possibilitaram determinar C. lamprauges como o agente etiológico da doença nos dois surtos aqui relatados.…”

Section: Históricounclassified

Surtos de conidiobolomicose ovina por Conidiobolus lamprauges no Estado da Bahia, Nordeste do Brasil

Peixoto¹,

Lima²,

Farias³

et al. 2017

Braz. J. Vet. Med.

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Section: Históricounclassified

Surtos de conidiobolomicose ovina por Conidiobolus lamprauges no Estado da Bahia, Nordeste do Brasil

Peixoto¹,

Lima²,

Farias³

et al. 2017

Braz. J. Vet. Med.

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“…The immunohistochemistry technique was performed as described by Galiza et al (2014), with some modifications: we used the labeled streptavidin biotin (LSAB) alkaline phosphatase technique (Dako Corporation, USA). The histological sections with 4 μm fixed on silanized slides were dewaxed and hydrated.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Nunes

Silva²,

Oliveira³

et al. 2015

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate occurrence of Toxoplasma gondii in sheep slaughtered in the state of Alagoas, Brazil, by means of different diagnosis techniques. Serum samples and tissues from 100 slaughtered sheep were used. To detect antibodies, the indirect immunofluorescence antibody test (IFAT) was used, and tissues from seropositive animals (cut-off ≥1:64) were submitted to Polymerase Chain Reaction (PCR) and immunohistochemistry (IHC). To assess the concordance between the direct techniques, the kappa test was used. In the IFAT, it was observed that 14% (14/100) of the ovine samples were serum-positive. In the PCR, 21.43% (3/14) of the animals were positive and in IHC, it was observed that 7.14% (1/14) were positively stained for T. gondii in cerebral tissue. Histopathologically, the predominant finding was the presence of mononuclear cell infiltrate in the heart and a perivascular cuff in the cerebrum and cerebellum. The concordance between the direct diagnosis techniques was moderate (k=0.44). Thus, it is important to use different direct techniques in diagnosing toxoplasmosis in naturally infected sheep.

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“…hyphae [11]. Therefore, as in this case, the confirmation of the etiology can be made by IHC using anti-P. insidiosum specific antibody, which has been utilized reliably since 1988 [3] and is well established by different authors [4][5][6]11].…”

Section: Discussionmentioning

confidence: 91%

Segmental Enteritis Associated with Pythium insidiosum Infection in a Horse

Silva

Flores

Lamego

et al. 2020

Acta Scientiae. Vet.

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Background: Pythiosis is an infectious disease caused by the oomycete Pythium insidiosum, with higher occurrence in wetlands and hot climate regions. This microorganism develops its cycle in aquatic plants, and most cases happen because of the contact of animals or people with water containing the motile zoospores (infectious form). Horses are the principal species affected and develop principally cutaneous and subcutaneous lesions, but the gastrointestinal tract is seldom affected. Humans develop various forms of pythiosis, such as a vascular form. The objectives of the current study are to describe an unusual case of intestinal pythiosis, its clinical signs, aspects of pathogenesis, and diagnosis.Case: A 13-year-old Crioula mare, from Santa Maria, RS, Brazil, presented with reduced food and water intake, apathy, restlessness, rolling, nasal reflux, firm abdomen upon palpation, and tachypnea during 2 days. The horses of this farm were fed native pasture and horse feed, and they had access to a nearby pond. Two days following the start of the clinical signs, the horse died and was necropsied on the farm. During necropsy, there was around 400 mL of reddish effusion in the abdominal cavity (modified transudate). A 15 cm segment of jejunum was firm upon palpation and had a severe transmural thickening. The wall of the affected area was up to 3 cm in thickness and firm, with small yellowish and irregular masses that stood out and looked friable, interpreted as kunkers. Microscopically, the yellowish masses (kunkers) were characterized by dense accumulations of intact and degenerate eosinophils (eosinophilic necrosis). Within these kunkers, and also on their periphery, there were multiple negatively stained hyphal profiles. Hyphae were also seen on the wall of small arteries inside the kunkers. These hyphae had nearly parallel walls and were occasionally septate, with a diameter of about 10 μm. The hyphae stained black with Grocott’s methenamine silver stain (GMS) and were positive on immunohistochemistry (IHC) using specific anti-P. insidiosum polyclonal antibody.Discussion: Colic is the principal clinical presentation in horses suffering from gastrointestinal tract disorders. In spite of the presence of colic in this horse, intestinal pythiosis was not clinically suspected in this case, principally because of its rare occurrence in horses. Even though it is persistent to observe horses ingesting water with suspected contamination by P. insidiosum, the cutaneous form of pythiosis is a lot more common than the intestinal form. This is the only case of intestinal pythiosis in a horse in 52 years of routine diagnosis in our laboratory. It is suspected that this horse got infected by drinking contaminated water from the nearby pond and microlesions in the intestinal mucosa due to plant material or some unknown pathogen may have favored zoospore adhesion, encysting and starting the colonization of the tissue by emitting a germ tube. A macroscopic observation of kunkers in the intestine is rare, but when observed it is highly indicative of pythiosis. The immunohistochemistry technique using anti-P. insidiosum antibody, in accordance with the literature, validates the diagnosis of pythiosis. In this case, the presence of hyphae in the blood vessel wall inside the kunkers propose that this finding may be involved in the spread of the lesion but needs more detailed studies.

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Utilização de três métodos imuno-histoquímicos na detecção de aspergilose e zigomicose em animais

Cited by 6 publications

References 20 publications

Surtos de conidiobolomicose ovina por Conidiobolus lamprauges no Estado da Bahia, Nordeste do Brasil

Surtos de conidiobolomicose ovina por Conidiobolus lamprauges no Estado da Bahia, Nordeste do Brasil

Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Segmental Enteritis Associated with Pythium insidiosum Infection in a Horse

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