Uso do plasma rico em plaquetas no reparo de úlceras de córnea profundas induzidas em coelhos. Avaliação clínica e histomorfométrica

Donatti, Camila I.; Brandão, Cláudia Valéria Seullner; Ranzani, José Joaquim Titton; Perches, Cintia Sesso; Padovani, Carlos Roberto; Pellizzon, Cláudia Helena; Sereno, María Guadalupe

doi:10.1590/s0102-09352013000300029

Cited by 9 publications

(10 citation statements)

References 15 publications

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“…The first centrifugation was performed at 200 G for 10 minutes in a centrifuge refrigerated at 22 o C (Hermle Z326K, Hermle Labortechnik GmbH, Wehingen, Baden-Württemberg, Germany). After the first centrifugation, 50% of the supernatant was discarded and the remaining amount was placed in another tube for additional centrifugation at 400 G for 10 minutes (Donatti et al 2013). Finally, approximately two thirds of the supernatant was discarded and the remaining one third was homogenized and designated as PRP, consisting of minimum increase of four times and average final concentration of 1.511.392 platelets (variation of 1.140.210 to 2.247.850 platelets).…”

Section: Methodsmentioning

confidence: 99%

Autologous and homologous skin grafts treated with platelet-rich plasma (PRP): experimental study in rabbits

et al. 2018

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ISSN 0100-736X (Print) ISSN 1678-5150 (Online) RESUMO.-[Enxertos cutâneos autólogos e homólogos tratados com plasma rico em plaquetas (PRP): estudo experimental em coelhos.] Objetiva-se com o presente estudo comparar a reparação tecidual de defeitos cutâneos em coelhos, submetidos a enxertos autólogos e homólogos, tratados ou não com plasma rico em plaquetas (PRP). Para isso, foram selecionados nove coelhos e constituídos quatro grupos experimentais, designados como G1, no qual foi realizado enxerto autólogo tratado com PRP; G2, enxerto autólogo; G3, enxerto homólogo tratado com PRP; e G4, enxerto homólogo. Foram realizadas avaliações macroscópica e histomorfométrica, por meio das colorações de Hematoxilina/Eosina e Tricômio de Masson, incluindo quantificação de fibras colágenas, contagem de macrófagos, fibroblastos e vasos. O uso do enxerto autólogo com PRP influenciou positivamente na fase inicial do processo de reparação tecidual à avaliação macroscópica, caracterizada por coloração rósea e de aspecto cosmético. À avaliação histomorfométrica, não houve diferença estatística quanto ao número de macrófagos e fibroblastos entre os enxertos tratados ou não com o PRP, bem como quanto às contagens de vasos e a quantificação das fibras colágenas. Conclui-se que o PRP sob a promoveu influência positiva na fase inicial ou de "pega" do enxerto autólogo.ABSTRACT.-Kemper B., Brandão C.V.S.The aim of the present study was to compare tissue repair of skin defects in rabbits submitted to autologous and homologous grafts treated or not with platelet-rich plasma (PRP). We selected nine rabbits and constituted four groups, designated as G1, in which were performed autologous graft treated with PRP; G2, autologous graft only; G3, homologous graft treated with PRP; and G4, homologous graft only. Macroscopic and histomorphometric evaluation was realized. The histomorphometric evaluation was performed by Hematoxylin/Eosin and Masson´s Trichrome staining with quantification of collagen fibers, macrophages, fibroblasts and vessels. The autologous graft treated with PRP showed positive influence on the early stage of the tissue repair process at the macroscopic evaluation, characterized by rosy color and cosmetic appearance. At the histomorphometric evaluation, there was no statistical difference in the number of macrophages and fibroblasts between the treated grafts or not with the PRP, as well as the quantification of vessels and collagen fibers. It can be concluded that PRP promotes a positive influence on the initial phase or "take" of the graft.

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Section: Methodsmentioning

confidence: 99%

Autologous and homologous skin grafts treated with platelet-rich plasma (PRP): experimental study in rabbits

et al. 2018

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“…Na periferia da córnea, não houve diferenças entre os grupos para a variável. (-26,67; 65,37) 37,82 (-29,53; 90,32) 38,95 (-33,35; 74,08) P>0,05 tampão 5,72 (-25,30; 237,18) -4,26 (-36,15; 360,89) 4,05 (-23,86; 295,48) (Donatti, 2010). No PRP, as plaquetas permanecem intactas e, assim, podem aderir à superfície ocular, atuando de forma biológica e mecânica; também secretam os fatores de crescimento que atuam sobre a superfície ocular, acelerando a reparação epitelial, além de modular a inflamação local e a angiogênese (Alió et al, 2012).…”

Section: Resultsunclassified

“…Há na literatura apenas um estudo experimental em coelhos, no qual se preconizou o tratamento com colírio à base de PRP e tampão sólido de PRP associado à membrana aminiótica em úlcera de córnea induzida em coelhos, e o resultado foi que os animais tratados apresentaram menor opacidade corneal e cicatrização epitelial mais rápida em relação ao grupo controle (Donatti, 2010).…”

Section: Introductionunclassified

Uso de plasma rico em plaquetas em úlceras de córnea em cães

Merlini¹,

Fonzar²,

Perches³

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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RESUMOEste trabalho teve por objetivo avaliar, clínica e macroscopicamente, o tratamento adjuvante com plasma rico em plaquetas na forma de colírio ou tampão, em úlceras de córnea de cães atendidos no Serviço de Oftalmologia Veterinária. Foram analisados 20 olhos com diagnóstico de ceratite ulcerativa, distribuídos em dois grupos experimentais. O grupo colírio (GC) foi constituído por olhos tratados topicamente com colírio autólogo de plasma rico em plaquetas (PRP), e o grupo tampão (GT) por olhos submetidos ao tratamento à base de tampão sólido de PRP, associado ao recobrimento com terceira pálpebra para retenção deste. Os grupos foram avaliados, por meio de avaliação clínica, macroscópica e análise da redução do defeito epitelial, em diferentes momentos, aos três, cinco, dez, 15 e 30 dias, com exceção do terceiro dia no GT. O recobrimento da terceira pálpebra foi removido no quinto dia no GT. Em ambos os grupos, houve redução dos sinais de inflamação, melhora na sensibilidade ocular e adequada reparação do defeito epitelial. Todos os olhos do GT apresentaram completa cicatrização no quinto dia e 70% no GC, atingindo a totalidade no 10º dia. O PRP na forma de colírio ou tampão é uma excelente terapia adjuvante a ser instituída no tratamento clínico da úlcera de córnea em cães, pois atua na diminuição dos sinais inflamatórios, da dor ocular e auxilia potencialmente na cicatrização do defeito epitelial. , at different times, at three, five, ten, 15 and 30 days, except Palavras-chave: cão, defeito epitelial, fatores de crescimento, PRP ABSTRACT This study aims to clinically and macroscopically evaluate the adjuvant therapy with platelet-rich plasma in the form of eyedrops or clot, for corneal ulcers in dogs treated at the Veterinary Ophthalmology Service. We analyzed 20 eyes diagnosed with ulcerative keratitis, divided into two experimental groups. The eyedrop group (GC) was composed of eyes treated topically with eyedrops of autologous plateletrich plasma (PRP), and the clot group (GT) was composed of eyes treated with a platelet-rich clot and covered with a third eyelid for retention of the clot. The groups were evaluated by clinical and macroscopic analysis and by the analysis of epithelial defect reduction

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“…In one human study, PRP was used as eye drops, the healing was improved significantly, and complete healing was possible in most of the participant cases (11). The eyes' position in the body makes them continuously exposed to numerous agents and traumatic events (50). These circumstances make them react to any insult constrained and may lead to vision loss (51).…”

Section: Etiological Agentsmentioning

confidence: 99%

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

et al. 2021

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Background: Corneal ulcer could be a major source of distress in small animals, with many contributing agents. In recent years, few studies evaluated the efficacy of platelet-rich plasma (PRP) in healing corneal ulcers.Aim: This study aimed to assess the ability of subconjunctival injection of autologous PRP in the treatment of corneal ulcers in dogs and cats as well as estimate the expression of matrix metalloproteinase (MMP)-2, MMP-9, and oxidative stress biomarkers in these patients.Methods: A total number of 28 animals (16 cats and 12 dogs) were enrolled in this study. Each animal was subjected to clinical, neurologic, and ophthalmic examinations where the type of ulcer was documented. Tear samples were collected for evaluation of oxidative biomarkers and MMPs; conjunctival swabs were taken to identify the involved organism. PRP was prepared from each animal and given as subconjunctival injection; numbers of injections were done according to case response. Clinical follow-up was done and documented for each case.Results: In cat patients, female and Persian cats were most affected; unilateral and superficial ulcers were most recorded. In male dogs, unilateral, and superficial ulcers were most recorded. FHV-1 was most identified in cats, while Staphylococcus aureus was most identified in dogs. Numbers of injections needed to achieve healing were recorded, with 50% of dogs needing two injections with 1-week intervals and 50% of cats needed three injections with 1-week intervals. Alterations in both oxidative biomarkers and MMPs were recorded in affected animals.Conclusion: The use of autologous PRP as a subconjunctival injection in treating corneal ulcers in dogs and cats is effective. The number of injections is the case and corneal ulcer type-dependent.Clinical Significance: Autologous PRP as a subconjunctival injection in treating corneal ulcer is a relatively cheap, safe method and can be done in the clinical setting.

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Uso do plasma rico em plaquetas no reparo de úlceras de córnea profundas induzidas em coelhos. Avaliação clínica e histomorfométrica

Cited by 9 publications

References 15 publications

Autologous and homologous skin grafts treated with platelet-rich plasma (PRP): experimental study in rabbits

Autologous and homologous skin grafts treated with platelet-rich plasma (PRP): experimental study in rabbits

Uso de plasma rico em plaquetas em úlceras de córnea em cães

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

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