Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

Farghali, Haithem A.; AbdElKader, Naglaa A.; AbuBakr, Huda O.; Ramadan, Eman; Khattab, Marwa S.; Salem, Noha Y.; Emam, Ibrahim A.

doi:10.3389/fvets.2021.641265

Cited by 27 publications

(33 citation statements)

References 81 publications

(147 reference statements)

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“…Among the orthobiologic blood products, particularly platelet products are already frequently used in clinical practice. In canine medicine, PRP or platelet concentrates have been used to treat several conditions, including osteoarthritis [ 17 , 18 , 19 , 20 ], lumbosacral stenosis [ 21 ], wounds [ 22 , 23 ], corneal ulcera [ 24 ], and aural hematoma [ 25 ]. While clinical benefits were shown, a major disadvantage of platelet concentrate is its limited long-term storage, as it cannot be frozen [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Hagen

Holland²,

Brandt³

et al. 2022

Animals

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Platelet lysate (PL) is an attractive platelet-based therapeutic tool and has shown promise as xeno-free replacement for fetal bovine serum (FBS) in human and equine mesenchymal stromal cell (MSC) culture. Here, we established a scalable buffy-coat-based protocol for canine PL (cPL) production (n = 12). The cPL was tested in canine adipose MSC (n = 5) culture compared to FBS. For further comparison, equine adipose MSC (n = 5) were cultured with analogous equine PL (ePL) or FBS. During canine blood processing, platelet and transforming growth factor-β1 concentrations increased (p < 0.05 and p < 0.001), while white blood cell concentrations decreased (p < 0.05). However, while equine MSC showed good results when cultured with 10% ePL, canine MSC cultured with 2.5% or 10% cPL changed their morphology and showed decreased metabolic activity (p < 0.05). Apoptosis and necrosis in canine MSC were increased with 2.5% cPL (p < 0.05). Surprisingly, passage 5 canine MSC showed less genetic aberrations after culture with 10% cPL than with FBS. Our data reveal that using analogous canine and equine biologicals does not entail the same results. The buffy-coat-based cPL was not adequate for canine MSC culture, but may still be useful for therapeutic applications.

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Section: Introductionmentioning

confidence: 99%

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Hagen

Holland²,

Brandt³

et al. 2022

Animals

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“…As plaquetas aderem na lesão, liberam citocinas e fatores de crescimento que estimulam a mitose de fibroblastos e, consequentemente, produção de colágeno (Merlini et al, 2014). O PRP tem fatores de crescimento, citocinas e integrinas (Farghali et al, 2021). É uma forma de tratamento não imunológica, econômica e pode ser aplicada logo após a coleta.…”

Section: Terapiasunclassified

“…O estudo de Farghali e colaboradores (2021) revelou que número de aplicações de PRP é dependente da espécie e morfologia da úlcera. A média de aplicações nos cães foi de duas injeções subconjuntivais com diferença de uma semana cada, já em felinos aplicadas três (Farghali et al, 2021). Já no trabalho de Merlini e colaboradores (2014) foi dividido em dois grupos, um utilizou tratamento tópico com colírio de PRP (autógeno) e outro tampão com recobrimento da terceira pálpebra.…”

Section: Terapiasunclassified

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Causas e correções da úlcera de córnea em animais de companhia – Revisão de literatura

Marcon

Sapin

2021

RSD

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Recentemente, a oftalmologia tem crescido progressivamente na medicina veterinária, resultando em novas descobertas das causas e dos tratamentos. A úlcera de córnea é o rompimento da membrana epitelial e o aparecimento do estroma, que é corado através da fluoresceína sódica, usado para diagnóstico da enfermidade. Tem diversos fatores condicionantes para a ocorrência. Esta revisão bibliográfica tem por objetivo reforçar aos estudantes e clínicos veterinários as causas de úlcera de córnea, a terapia mais indicada para cada caso e listar os possíveis tratamentos e defini-los. O presente estudo envolve técnicas de tratamento de úlcera de córnea. Foram utilizados artigos em bases de dados no período de 2006 a 2021, além de consultas em livros de oftalmologia veterinária. O animal com úlcera de córnea deve passar por exame clínico completo, específico e complementares, recomenda-se realizar cultura e antibiograma para adequar a terapia. Cabe ao clínico investigar os fatores que predispõe e o melhor tratamento, da mesma forma, o cirurgião deve determinar a melhor técnica conforme indicações e habilidades próprias. Novos tratamentos precisam de mais estudos sobre aplicabilidade. Mas os tratamentos cirúrgicos geralmente continuam sendo seguros e eficazes principalmente nos casos graves.

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“…In veterinary medicine, PRPs are mainly used in the treatment of musculoskeletal, ophthalmological and orthopedic pathologies [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Platelet-Rich Plasma for the Treatment of Degenerative Lumbosacral Stenosis: A Study with Retired Working Dogs

Hernández-Guerra

Carrillo

Sopena

et al. 2021

Animals

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Traditionally, canine degenerative lumbosacral stenosis (DLS) has been defined as a multifactorial syndrome characterized by lumbosacral pain triggered by the compression of the nerve rootlets of the cauda equina. There is still no consensus on the treatment of this condition, probably because there are a plethora of possible causes. In addition to compression, inflammation is a very important factor in the physiopathology of the disorder. Platelet-rich plasma (PRP) consists of an increased concentration of autologous platelets suspended in a small amount of plasma. Platelets are a source of several growth factors. Growth factors were shown to help in wound healing and biological processes, such as chemotaxis, neovascularization and synthesis of extracellular matrix, and growth factors were used to improve soft tissue healing and bone regeneration. PRP also facilitates the restoration of the structural integrity of the affected anatomy. Fourteen dogs diagnosed with DLS were treated with three epidural injections of PRP on days 0, 15 and 45. All dogs showed clinical improvement 3 months after the initial treatment. Gait was also objectively assessed by means of the use of force platform analysis before and after treatment, showing significant improvement. The results show that PRP may provide a good alternative to other nonsurgical treatments, such as prednisolone epidural injection.

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Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

Cited by 27 publications

References 81 publications

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same

Causas e correções da úlcera de córnea em animais de companhia – Revisão de literatura

Platelet-Rich Plasma for the Treatment of Degenerative Lumbosacral Stenosis: A Study with Retired Working Dogs

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