Using nutritional and oxidative stress to increase content of healthbeneficial fatty acids in oleaginous and non-oleaginous yeasts

Pádrová, Karolína; Kolouchová, Irena; Řezanka, Tomáš; Čejková, Alena

doi:10.1515/chempap-2016-0060

Cited by 2 publications

(1 citation statement)

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“…Nutrient deficiencies, such as nitrogen or phosphate limitations, coupled with high carbon abundance have been shown to trigger lipid accumulation in oleaginous yeast and certain conventional yeast species [73,74]. Furthermore, oxidative stress can induce changes in the unsaturated fatty acid content as a protective response against ROS damage [75]. Although several biotechnologically relevant yeast species have been investigated for POA synthesis under various cultivation conditions, P. pastoris was not among the organisms studied [74].…”

Section: Analysis Of Growth Conditions and Nutrient Manipulationmentioning

confidence: 99%

Tuning Fatty Acid Profile and Yield in Pichia pastoris

Kobalter,

Voit,

Bekerle-Bogner

et al. 2023

Bioengineering

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Fatty acids have been supplied for diverse non-food, industrial applications from plant oils and animal fats for many decades. Due to the massively increasing world population demanding a nutritious diet and the thrive to provide feedstocks for industrial production lines in a sustainable way, i.e., independent from food supply chains, alternative fatty acid sources have massively gained in importance. Carbohydrate-rich side-streams of agricultural production, e.g., molasses, lignocellulosic waste, glycerol from biodiesel production, and even CO2, are considered and employed as carbon sources for the fermentative accumulation of fatty acids in selected microbial hosts. While certain fatty acid species are readily accumulated in native microbial metabolic routes, other fatty acid species are scarce, and host strains need to be metabolically engineered for their high-level production. We report the metabolic engineering of Pichia pastoris to produce palmitoleic acid from glucose and discuss the beneficial and detrimental engineering steps in detail. Fatty acid secretion was achieved through the deletion of fatty acyl-CoA synthetases and overexpression of the truncated E. coli thioesterase ‘TesA. The best strains secreted >1 g/L free fatty acids into the culture medium. Additionally, the introduction of C16-specific ∆9-desaturases and fatty acid synthases, coupled with improved cultivation conditions, increased the palmitoleic acid content from 5.5% to 22%.

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