Using Biferon-B for the prevention of mastitis in cows

Abstract

The developed method for the prevention of mastitis in lactating cows by intramuscular injection of Biferon-B at doses of 5.0–10.0–20.0 ml, provided a preventive effect in 25.0–75.0 % of animals; the best effect was achieved with Biferon-B at a dose of 5.0 ml. The use of Biferon-B in clinically healthy lactating animals was accompanied by a decrease in blood levels of stab neutrophils by 58.8–65.0 %, circulating immune complexes by 23.4–62.6 %, with a higher content of segmented neutrophils by 4.8–7.8 %, monocytes by 5.6–57.1 %, lymphocytes by 4.9–12.3 %, total immunoglobulins by 5.7–14.3 %, bactericidal and lysozyme activity of blood serum by 6.4–23.1 % and 4.3–13.7 %, respectively. The phagocytic activity of neutrophils decreased by 5.6–10.9 %. With lower indicators of the intoxication index dropped by 12.8–19.1 %, the content of average molecular peptides by 2.4–22.6 %, nitric oxide by 24.5–45.4 %, MDA by 2.8–36.7 %; catalase activity increased by 2.7–12.8 % and GPx by 10.4–29.7 %.

Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics

“…However, we managed to find two works dedicated to in vivo mastitis treatments. Shabunin et al (2020) [31] evaluated the effect of Biferon-B (the drug, exhibiting antiviral and immunostimulant activity, which presents a mixture of bovine recombinant a-and y-interferons with species specificity) on the prevention of mastitis in cows by intramuscular injection of Biferon-B. Biferon-B was able to increase lysozyme activity by 4.3-13.7%.…”

“…This increase was less than in our case (20.5%). The methodology reported led to preventive effect in 25.0-75.0% of the cows [31]. Unfortunately, the drug showed an alteration effect on immunological cells such as neutrophils (~59-65%), monocytes (~6-57%), and lymphocytes (~5-12%) [31].…”

“…The methodology reported led to preventive effect in 25.0-75.0% of the cows [31]. Unfortunately, the drug showed an alteration effect on immunological cells such as neutrophils (~59-65%), monocytes (~6-57%), and lymphocytes (~5-12%) [31]. Gao et al (2017) [13] showed that the baicalin bioactive flavonoid binds to lysozyme increasing its activity against S. aureus isolated from mice with mastitis.…”