Using an Electrical Potential to Reversibly Switch Surfaces between Two States for Dynamically Controlling Cell Adhesion

Ng, Cheuk Chi Albert; Magenau, Astrid; Ngalim, Siti Hawa; Ciampi, Simone; Chockalingham, Muthukumar; Harper, Jason B.; Gaus, Katharina; Gooding, J. Justin

doi:10.1002/anie.201202118

Cited by 124 publications

(82 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Electrical potentials have been more widely used to alter biointerface properties; their effects range from reorganisation of the molecular conformation of molecules on the surface 85,98,109 to the induction of chemical reactions that modulate the presence of surface chemical groups. 110,[120][121][122] These materials rely on the presence of an electrically conducting substrate material.…”

Section: External Stimulimentioning

confidence: 99%

“…By dynamically changing the presentation of RGD based peptides on the surface, the adhesion of osteoblasts, 74,87 fibroblasts, 86,108,110,113,123,127 endothelial 109 and HeLa cells 88,112 has been reported. In several cases, cell adhesiveness of the surface could be changed repeatedly and reversibly.…”

Section: Biological Responsementioning

confidence: 99%

“…In several cases, cell adhesiveness of the surface could be changed repeatedly and reversibly. [86][87][88]109,111,112,127 In analogy to the pNIPAM based thermo-responsive surfaces that were designed to harvest cell sheets, 136,137 many of these cell-adhesion modulating surfaces were put forward as alternative routes to harvest cells. Notable other applications focus on the ability to spatially control the adhesion of cells to enable co-cultures of different cell types in predefined patterns 81 .…”

Section: Biological Responsementioning

confidence: 99%

“…As this comprises signals that are readily controlled by a human or machine, it is the most prevalent way that surface responses have been triggered to date. Figure 3 shows a representation of external stimuli; artificial changes in temperature, 91,92,107 the application of an electrical potential, 85,[108][109][110] the application of light 87,88,111,112 and mechanical forces 93,113 have all been used to trigger a dynamic change in either chemical or physical surface properties to affect the behaviour of cells. In addition, biomolecules that are naturally present in a biological environment -enzymes 74,76 and carbohydrates 89 for example -have been artificially added to systems to trigger a response of the biointerface.…”

Section: External Stimulimentioning

confidence: 99%

“…To control the display of a peptide (GRGDS) on a surface, the same electrochemically controlled mechanism was employed in a mixed SAM that contained peptide conjugates and molecules with charged endgroups (sulfates and tertiary amines). 109 In the absence of an electrical potential, the charged molecules were extended and shielded the peptides from cells cultured on these surfaces, preventing cell adhesion. Application of an electrical potential caused the charged molecules to reorient towards the surface, making the peptide available for cellular interaction and promoting the adhesion of endothelial and differentiated HL60 cells.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Regulation of Stem Cell Fate by Nanomaterial Substrates

Mashinchian

Turner

Dalby

et al. 2015

Nanomedicine (Lond.)

View full text Add to dashboard Cite

Major design aspects for novel biomaterials are driven by the desire to mimic more varied and complex properties of a natural cellular environment with man-made materials. The development of stimulus responsive materials makes considerable contributions to the effort to incorporate dynamic and reversible elements into a biomaterial. This is particularly challenging for cell-material interactions that occur at an interface (biointerfaces); however, the design of responsive biointerfaces also presents opportunities in a variety of applications in biomedical research and regenerative medicine. This review will identify the requirements imposed on a responsive biointerface and use recent examples to demonstrate how some of these requirements have been met. Finally, the next steps in the development of more complex biomaterial interfaces, including multiple stimuli responsive surfaces, surfaces of 3D objects and interactive biointerfaces will be discussed.

show abstract

Section: External Stimulimentioning

confidence: 99%

Section: Biological Responsementioning

confidence: 99%

Section: Biological Responsementioning

confidence: 99%

Section: External Stimulimentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Regulation of Stem Cell Fate by Nanomaterial Substrates

Mashinchian

Turner

Dalby

et al. 2015

Nanomedicine (Lond.)

View full text Add to dashboard Cite

show abstract

Stimuli‐Responsive Surfaces in Biomedical Applications

Pranzetti¹,

Preece²,

Mendes³

2013

Intelligent Stimuli‐Responsive Materials

View full text Add to dashboard Cite

Nature provides mechanisms that are able to dynamically control specific and nonspecific interactions between cells and biological surfaces [1,2]. Scientists have long tried to reproduce these dynamic biological events and have recently made an important step in that direction by creating artificial stimuli-responsive surfaces [3][4][5][6][7]. These smart substrates present modulatory surface properties that are able to respond to external chemical/biochemical [8][9][10][11][12], thermal [13][14][15], electrical [16][17][18][19][20], and optical stimuli [21][22][23][24][25][26][27][28][29][30][31]. Due to their dynamic nature such substrates are very appealing for applications in the biomedical field [32]. Progress to date has led to control over biomolecule activity [33] and immobilization of a diverse array of proteins, including enzymes [34] and antibodies [35]. These prior achievements have encouraged researchers to take the challenge of using dynamic surfaces to modulate larger and more complex systems, such as bacteria [36] and mammalian cells [37].Achieving control over surface properties could provide new insights in the understanding of cell behavior and can offer distinct benefits with regard to the development of medical devices. For instance, the modulation of cell attachment and detachment could lead to the prevention of unwanted bacteria fouling on implants, reducing the risk of infections and rejection [38][39][40][41][42]. Furthermore, dynamic surfaces able to present on demand regulatory signals to a cell could provide unprecedented opportunities in studies of cell responses in real-time. Cells in tissues adhere to and interact with their extracellular environment via specialized cell-cell and cell-extracellular

show abstract