Regulation of Stem Cell Fate by Nanomaterial Substrates

Mashinchian, Omid; Turner, Lesley-Anne; Dalby, Matthew J.; Laurent, Sophie; Shokrgozar, Mohammad Ali; Bonakdar, Shahin; Imani, Mohammad; Mahmoudi, Morteza

doi:10.2217/nnm.14.225

Cited by 65 publications

(45 citation statements)

References 320 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the fact that surface topography and elasticity affect cell fate [26][27][28] 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 chondrocytes imprinted substrates ( figure 5-a2). The same results were also previously reported for rabbit stem cells 19 .…”

Section: Discussionmentioning

confidence: 99%

Cell-Imprinted Substrates Modulate Differentiation, Redifferentiation, and Transdifferentiation

Bonakdar

Mahmoudi

Montazeri

et al. 2016

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Differentiation of stem cells into mature cells through the use of physical approaches is of great interest. Here, we prepared smart nanoenvironments by cell-imprinted substrates based on chondrocytes, tenocytes, and semifibroblasts as templates and demonstrated their potential for differentiation, redifferentiation, and transdifferentiation. Analysis of shape and upregulation/downregulation of specific genes of stem cells, which were seeded on these cell-imprinted substrates, confirmed that imprinted substrates have the capability to induce specific shapes and molecular characteristics of the cell types that were used as templates for cell-imprinting. Interestingly, immunofluorescent staining of a specific protein in chondrocytes (i.e., collagen type II) confirmed that adipose-derived stem cells, semifibroblasts, and tenocytes can acquire the chondrocyte phenotype after a 14 day culture on chondrocyte-imprinted substrates. In summary, we propose that common polystyrene tissue culture plates can be replaced by this imprinting technique as an effective and promising way to regulate any cell phenotype in vitro with significant potential applications in regenerative medicine and cell-based therapies.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell-Imprinted Substrates Modulate Differentiation, Redifferentiation, and Transdifferentiation

Bonakdar

Mahmoudi

Montazeri

et al. 2016

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because adherent cells attach loosely to the uncharged polystyrene plate, its surface must be modified by plasma, for example. However, its rigidity is not ideal for stem cell growth, often inducing random and spontaneous differentiation (Mashinchian et al 2015). In our work, PCL nano-/microfibers had good physicomechanical properties compared to TCP, and this accounted for significant keratinocyte differentiation and higher proliferative activity of ASCs on the PCL matrix.…”

Section: Discussionmentioning

confidence: 67%

“…In the present study, it seemed that ASCs might have considerable impact on epithelialization, development of skin appendages and organization of the dermis and eventually accelerate wound healing compared to the application of PCL matrices devoid of ASCs after implantation. Mashinchian et al (2015) have introduced chemical, physicomechanical and topographical factors as a triangle of control of stem cell fate. Although chemical factors are potent regulators of cell behavior, their effects are limited because they are thought to be only one component of the 'triangle' of cell control.…”

Section: Discussionmentioning

confidence: 99%

A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study

et al. 2016

View full text Add to dashboard Cite

Polycaprolactone (PCL) is used as a material of choice for surgical sutures, wound dressings, contraceptives, fixation devices and dentistry in paramedical sciences. In addition, adipose-derived stem cells (ASCs) have been shown to be effective in the treatment of acute and chronic wounds. This study aimed to evaluate the effect of electrospun PCL fibers on keratinocyte differentiation of ASCs and wound healing. PCL solution was electrospun and characterized. Isolated and characterized ASCs were differentiated into keratinocyte-like cells on a tissue culture plate (TCP) and PCL matrices and compared. PCL nano-/microfibers cultured with ASCs (test group) or alone (control) were implanted as a dermal substitute for wound healing. There were significant increases in the proliferation rate and expression level of cytokeratin 14, filaggrin and involucrin in cells cultured on PCL matrices compared to TCP (p < 0.05). After histological and immunological evaluation of the reconstituted skin, a thick epidermal layer with several skin appendages was evidently observed in the ASC/PCL group, whereas no real and mature epidermis was formed, especially in the central area of the healing wound in the pure PCL group on day 14. Pure PCL, if possessing suitable properties including good adhesiveness, high proliferative capability, inductive elasticity and stiffness for migration and differentiation, could drive the keratinocyte differentiation of ASCs and act as an efficient dermal equivalent to promote wound healing.

show abstract

“…Current micro and nanofabrication techniques can be implemented to generate 2D and 3D structures possessing the topography and surface features to support more physiologically relevant 3D stem cell cultures [103,104]. Among such fabrication techniques is electrospinning.…”

Section: D Substrates For Psc Culturementioning

confidence: 99%

Commercializing Electrospun Scaffolds For Pluripotent Stem Cell-Based Tissue Engineering Applications

Mohtaram¹,

Karamzadeh

Shafieyan³

et al. 2017

Electrospinning

View full text Add to dashboard Cite

Tissue engineering, the process of combining bioactive scaffolds often with cells to produce replacements for damaged organs, represents an enormous market opportunity. This review critically evaluates the commercialization potential of electrospun scaffolds for applications in stem cell biology, including tissue engineering. First, it provides an overview of pluripotent stem cells (PSCs) and their defining properties, pluripotency and the ability to self-renew. These cells serve as an important tool for engineering tissues, including for clinical applications. Next, we review the technique of electrospinning and its promise for fabricating cell culture substrates and scaffolds for directing tissue formation from stem cells and compare these scaffolds to existing technologies, such as hydrogels. We address the associated market for electrospun scaffolds for PSCs and its potential for growth along with highlighting the importance of 3D cell culture substrates for PSCs by analyzing the net capital invested in this market and the associated growth rate. This review finishes by detailing the current state of commercializing electrospun scaffolds along with pathways for translating these scaffolds from research laboratories into successful start-up companies and the associated challenges with this process.

show abstract

Regulation of Stem Cell Fate by Nanomaterial Substrates

Cited by 65 publications

References 320 publications

Cell-Imprinted Substrates Modulate Differentiation, Redifferentiation, and Transdifferentiation

Cell-Imprinted Substrates Modulate Differentiation, Redifferentiation, and Transdifferentiation

A dermal equivalent developed from adipose-derived stem cells and electrospun polycaprolactone matrix: an in vitro and in vivo study

Commercializing Electrospun Scaffolds For Pluripotent Stem Cell-Based Tissue Engineering Applications

Contact Info

Product

Resources

About