Using a Biological Control Method For Controlling Red Spider Mite

Abstract

The journal of Toxicology and pest control is one of the series issued twice by the Egyptian Academic Journal of Biological Sciences, and is devoted to publication of original papers related to the interaction between insects and their environment. The goal of the journal is to advance the scientific understanding of mechanisms of toxicity. Emphasis will be placed on toxic effects observed at relevant exposures, which have direct impact on safety evaluation and risk assessment. The journal therefore welcomes papers on biology ranging from molecular and cell biology, biochemistry and physiology to ecology and environment, also systematics, microbiology, toxicology, hydrobiology, radiobiology and biotechnology. www.eajbs.eg.net Provided for non-commercial research and education use. Not for reproduction, distribution or commercial use.The study revealed the pathogenicity of entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin, Metarhizium anisopliae (Metschnikoff) against egg and adult stages of red spider mite Tetranychus urticae (Koch). Results showed that M. anisopliae was more pathogenic against egg stage than B. bassiana, while B. bassiana was more pathogenic against adult stage. The results of Scanning Electron Microscope (SEM) described the development cycle of B. bassiana on T. urticae.

Virulent Entomopathogenic Fungi against The Two-Spotted Spider Mite Tetranychus urticae and some Associated Predator Mites as Non Target Organisms

“…The cotton pads were moistened daily to avoid disc dryness, and to prevent mite escape. Mulberry leaves were changed by fresh one from time to time when necessary (Hassan, 2008;Sewify et al, 2015).…”

“…All the six tested entomopathogenic fungi were cultured on autoclaved sabouraud dextrose agar with yeast extract (SDAY) media (4 % Dextrose , 0.1 % peptone + 1.5 % Agar + 0.2 % yeast extract dissolved 70 % RH -C, 60 °1 ± edia were then incubated at 25 1L of Distilled Water). The m in and in darkness for 10-14 days to obtain conidia (Uma Devi et al, 2005;Sewify et al, 2015).…”

“…The spores were counted in the suspension using a heamocytometer (Neubauer improved HBG, Germany 0.100 mm 2 x 0.0025 mm 2 ) under a phasecontrast microscope. Five concentrations of each isolate were prepared: 10 6 , 5x10 6 , 10 7 , 5 x 10 7 , and 10 8 spore/ml, as well as the control (Distilled Water of 0.01% Tween 80) and were used on the same day after preparation and shaken before use (Hassan, 2008;Sewify et al, 2015).…”

“…Adult female were allowed to lay eggs freely for 24 hours to deposit egg then adults were removed from the disks and deposited egg were counted. The eggs were sprayed using direct spray technique (Abo-Shabana, 1980;Hassan, 2008;Sewify et al, 2015) by a glass atomizer at 30cm high with 2ml spore suspension for each treatment and 2ml sterilized distilled water of 0.01% Tween 80 as control.…”

“…Ten fertilized adult female of T. urticae were placed on a single leaf-disk of mulberry (2.5cm in diameter) and were kept on moist cotton wool in fiber dishes; each dish contained 5 disks as replicates. The direct spray technique was applied directly to the body surface of the mites according to Abo-Shabana (1980) and Sewify et al (2015).…”