Virulent Entomopathogenic Fungi against The Two-Spotted Spider Mite Tetranychus urticae and some Associated Predator Mites as Non Target Organisms

Hassan, Dalia; Rizk, Marguerite A.; Sobhy, Hassan M.; Mikhail, Wafai Z. A.; Nada, Maha

doi:10.21608/eajb.2017.12124

Cited by 7 publications

(7 citation statements)

References 30 publications

(41 reference statements)

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“…Regarding to the LT 90 , Bugeme et al (2009) reported values from 3.1 (ICIPE48) to 11.7 (ICIPE97) By other hand, the calculated LC 50 for the most pathogenic isolates (Ma110, Ma109 and Ma106) ranged from 1.1 × 10 8 to 1.2 × 10 8 conidia mL −1 . These values are similar to those reported by Hassan et al (2017), who documented LC 50 of 9.3 × 10 7 and 4.57 × 10 8 conidia mL −1 for M. anisopliae isolates on T. urticae. In contrast, the LC 50 values in the present study were relatively higher than the LC 50 values of 2.0 × 10 5 to 5.0 × 10 5 conidia mL −1 reported in previous studies by Elhakim et al (2020).…”

Section: Discussionsupporting

confidence: 91%

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

Basulto

Ruíz-Sánchez

Gómez

et al. 2022

Egypt J Biol Pest Control

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Background The two-spotted spider mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), is one of the most damaging mites in agriculture. Due to the concern for the intensive use of synthetic acaricides, entomopathogenic fungi represents a feasible alternative to T. urticae management. In the present study, 7 isolates of Metarhizium were characterized physiological and molecularly (based on the ITS1-5.8s-ITS2 rDNA) and evaluated for their acaricidal activity [mortality, mean and 90 lethal concentration (LC50: LC90) and mean and 90 lethal time (LT50: LT90)] against T. urticae under laboratory conditions. Results Sequencing of the ITS1-5.8s-ITS2 rDNA region indicated that the 7 isolates belong to M. anisopliae. The isolates Ma114 (3.7 ± 0.006 mm day−1), Ma109 (3.5 ± 0.009 mm day−1) and Ma106 (3.5 ± 0.006 mm day−1) had the highest radial growth rate and Ma114 (92.2 ± 0.86%) and Ma108 (94.4 ± 1.07%) had the highest germination percentage. All isolates were pathogenic to T. urticae, causing mortality that ranged from 45.3 to 85.3%. The LC50 and LC90 were 1.2 and 2.8, 1.1 and 2.5, and 1.2 and 2.8 × 108 conidia mL−1 for isolates Ma110, Ma109 and Ma106, respectively, while the LT50 and LT90 were 7.7 and 16.5, and 7.2 and 16.1 days for isolates M110 and Ma109, respectively. Conclusion The isolates Ma110 and Ma109 of M. anisopliae were moderately pathogenic and virulent against T. urticae.

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Section: Discussionsupporting

confidence: 91%

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

Basulto

Ruíz-Sánchez

Gómez

et al. 2022

Egypt J Biol Pest Control

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“…Several studies have shown that spider mites are susceptible to and affected by EPF. B. bassiana strains showed a high virulence for T. urticae eggs and adults (Hassan et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the entomopathogenic fungus, Beauveria bassiana and Lecanicillium muscarium against two main pests, Bemisia tabaci (Genn.) and Tetranychus urticae (Koch), under geothermal greenhouses of Southern Tunisia

Chouikhi

Assadi

Grissa-Lebdi

et al. 2022

Egypt J Biol Pest Control

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Background The geothermal greenhouses in Southern Tunisia are an important axis of agricultural development. This sector faces many abiotic and biotic constraints that could threat its sustainability. Thus, the heated greenhouses encounter destructive pests such as the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) and the two-spotted spider mite Tetranychus urticae (Koch) (Acari: Tetranychidae). Results This study aimed to assess the effect of the entomopathogenic fungi (EPF) Beauveria bassiana (strain ATCC and strain R444) and Lecanicillium muscarium strain Ve6 on simultaneous existence of T. urticae and B. tabaci in the host plants. The EPF had a significant effect on eggs and larvae of B. tabaci and on eggs and mobile forms of T. urticae in particular. The use of B. bassiana ATCC, B. bassiana R444 and L. muscarium strains Ve6 showed significant efficacies against B. tabaci larvae and eggs compared to untreated control. Indeed, the reduction percent of B. tabaci eggs varied between 42.65 and 58.52%. Thus, the efficacy against the number of B. tabaci larvae was in order to 65.04, 60.26 and 55.52% of B. bassiana strain ATCC, B. bassiana strain R444 and L. muscarium strain Ve6, respectively. In addition, these EPF were very effective on T. urticae eggs with a percentage reduction greater than 92.86%, whereas the percentage reduction in the T. urticae mobile forms varied between 95.11 and 98.52%. Conclusions Use of EPF will be an imperative to develop directed interventions at the integrated management of these two pests in protected and geothermal crops.

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“…Bugeme et al (2014) found the egg hatching rates of T. urticae to be 60.2%, 50.8%, 34.7%, 27.4%, 7 days after the application of 3x10 5 , 1x10 6 , 3x10 6 , and 1x10 7 conidia/ml concentrations of B. bassiana (ICIPE279) under laboratory conditions. Hassan et al (2017) investigated the effects of 10 6 , 10 7 , and 10 8 spore/ml doses of 4 different isolates (B1, B2, B3, B4) of B. bassiana on T. urticae eggs. 7 days after applying suspensions of B. bassiana isolates, the egg hatching rates of B1, B2, B3, and B4 isolates were 93.29, 81.67, 85.93, 80.2% for 10 6 spore/ml, 87.26, 58.75, 71.94, 36.38% for 10 7 spore/ml, 68.07, 33.9, 56.66, 25.2% for 10 8 spore/ml.…”

Section: Discussionmentioning

confidence: 99%

Effect of Native Beauveria bassiana Vuillemin Isolates on Egg Hatching of Tetranychus urticae Koch (Acari: Tetranychidae)

Uzun

2023

Black Sea Journal of Agriculture

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In this study, the effect of culture filtrates prepared at different doses of native Beauveria bassiana isolates (BIM-001, BY2, and GC8) on egg hatching of Tetranychus urticae Koch was determined. The adult females of T. urticae were transferred to bean leaves (4 cm) prepared according to the leaf disc method, as 10 individuals. After 24 hours, 20 eggs/leaf discs were prepared in each petri dish. Pure culture filtrates (1X) and other diluted doses (5X, 10X) were applied to leaf discs containing eggs for 10 seconds by spraying method. Observations were started 24 hours after the application and continued until the 7th day. Experiments were carried out with 5 replications for each dose of entomopathogen fungus isolates. The egg hatching of T. urticae was 19% at the pure culture filtrate dose of B. bassiana BIM-001 isolate (1X) 7 days after the application, and it was different and significant than the other isolates (P < 0.05). Egg hatching rates of T. urticae for BIM-001, BY2, and IGÇ isolates were determined between 19-38%, 32-48%, and 36-53%, respectively. These rates were found to be 31-38%, 43-48%, and 46-53% at 5X and 10X doses of BIM-001, BY2, and IGÇ isolates. There was no significant difference in egg hatching rates of pure culture filtrates of B. bassiana BY2 and IGÇ isolates (P ˃ 0.05).

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Virulent Entomopathogenic Fungi against The Two-Spotted Spider Mite Tetranychus urticae and some Associated Predator Mites as Non Target Organisms

Cited by 7 publications

References 30 publications

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

Physiological and molecular characterization of Metarhizium isolates and their acaricidal activity against Tetranychus urticae Koch (Trombidiformes: Tetranychidae)

Efficacy of the entomopathogenic fungus, Beauveria bassiana and Lecanicillium muscarium against two main pests, Bemisia tabaci (Genn.) and Tetranychus urticae (Koch), under geothermal greenhouses of Southern Tunisia

Effect of Native Beauveria bassiana Vuillemin Isolates on Egg Hatching of Tetranychus urticae Koch (Acari: Tetranychidae)

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