Usefulness of the murine model to study the immune response against Histoplasma capsulatum infection

Sahaza, Jorge H.; Pérez‐Torres, Armando; Zenteno, Edgar; Taylor, Mária Lucía

doi:10.1016/j.cimid.2014.03.002

Cited by 13 publications

(13 citation statements)

References 106 publications

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“…Experimental studies have also found differences in the virulence of the pathogen, gene expression, and pathogenesis of disease between H . capsulatum from different phylogenetic clades 35–37 . Despite this, associations between genetic populations and phenotypic features were not detected (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Novel clinical and dual infection by Histoplasma capsulatum genotypes in HIV patients from Northeastern, Brazil

Damasceno¹,

Teixeira

Barker

et al. 2019

Sci Rep

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Histoplasmosis is a worldwide-distributed deep mycosis that affects healthy and immunocompromised hosts. Severe and disseminated disease is especially common in HIV-infected patients. At least 11 phylogenetic species are recognized and the majority of diversity is found in Latin America. The northeastern region of Brazil has one of the highest HIV/AIDS prevalence in Latin America and Ceará State has one of the highest death rates due to histoplasmosis in the world, where the mortality rate varies between 33–42%. The phylogenetic distribution and population genetic structure of 51 clinical isolates from Northeast Brazil was studied. For that morphological characteristics, exoantigens profile, and fungal mating types were evaluated. The genotypes were deduced by a MSLT in order to define local population structure of this fungal pathogen. In addition, the relationships of H . capsulatum genotypes with clinically relevant phenotypes and clinical aspects were investigated. The results suggest two cryptic species, herein named population Northeast BR1 and population Northeast BR2. These populations are recombining, exhibit a high level of haplotype diversity, and contain different ratios of mating types MAT1-1 and MAT1-2 . However, differences in phenotypes or clinical aspects were not observed within these new cryptic species. A HIV patient can be co-infected by two or more genotypes from Northeast BR1 and/or Northeast BR2, which may have significant impact on disease progression due to the impaired immune response. We hypothesize that co-infections could be the result of multiple exposure events and may indicate higher risk of disseminated histoplasmosis, especially in HIV infected patients.

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Section: Discussionmentioning

confidence: 99%

Novel clinical and dual infection by Histoplasma capsulatum genotypes in HIV patients from Northeastern, Brazil

Damasceno¹,

Teixeira

Barker

et al. 2019

Sci Rep

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“…This pathogen is the etiologic agent of histoplasmosis, a respiratory and systemic mycosis with different clinical presentations, depending on the host’s susceptibility, fungal virulence, and other factors associated with the infection process. 1…”

Section: Introductionmentioning

confidence: 99%

“…Thus, because H. capsulatum conidia and hyphal fragments infect the host through the upper respiratory airway, the nose with the nasal mucosa (NM), including the nasal-associated lymphoid tissue (NALT) and the cervical lymph nodes (CLN), may be engaged during the first steps of the fungal morphotype’s transition, capture, and dissemination, which likely occur through the constitutive DCs, located strategically in the NM and the CLN. 1 This study aimed at determining the roles played by the NALT, the NM, and the CLN as the initial anatomic sites for H. capsulatum M-to-Y morphotype transition and primary dissemination during the early stages of H. capsulatum intranasal infection of two different mammalian hosts. Pioneering strategies were implemented using bats (natural wild hosts) and mice (laboratory hosts) as experimental models to identify each fungal morphotype in the host´s infected tissues.…”

Section: Introductionmentioning

confidence: 99%

Dimorphism and Dissemination of Histoplasma capsulatum in the Upper Respiratory Tract after Intranasal Infection of Bats and Mice with Mycelial Propagules

Suárez-Alvarez

Sahaza

Berzunza-Cruz

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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. This article describes, for the first time, the role of the nasal mucosa (NM) as the initial site for the Histoplasma capsulatum mycelial-to-yeast transition. The results highlight that yeasts may arrive to the cervical lymph nodes (CLN) via phagocytes. Bats and mice were intranasally infected with H. capsulatum mycelial propagules and they were killed 10, 20, and 40 minutes and 1, 2, and 3 hours after infection. The NM and the CLN were monitored for fungal presence . Yeasts compatible with H. capsulatum were detected within the NM and the CLN dendritic cells (DCs) 2–3 hours postinfection, using immunohistochemistry. Histoplasma capsulatum was re-isolated by culturing at 28°C from the CLN of both mammalian hosts 2–3 hours postinfection. Reverse transcription-polymerase chain reaction assays were designed to identify fungal dimorphism, using mycelial-specific ( MS8 ) and yeast-specific ( YPS3 ) gene expression. This strategy supported fast fungal dimorphism in vivo, which began in the NM 1 hour postinfection (a time point when MS8 and YPS3 genes were expressed) and it was completed at 3 hours (a time point when only the YPS3 transcripts were detected) in both bats and mice. The presence of intracellular yeasts in the nasal-associated lymphoid tissue (NALT), in the NM nonassociated with the NALT, and within the interdigitating DCs of the CLN suggests early fungal dissemination via the lymph vessels.

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“…The distribution of parasitic wasps is almost nationwide, distributed in northern China, namely in the Jilin, Hebei, Shanxi and Henan Provinces [ 35 ]. Parasitoids have been broadly studied in China [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Population dynamics and species composition of maize field parasitoids attacking aphids in northeastern China

et al. 2020

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Maize, Zea mays L., is the most abundant field crop in China. Aphids are the most economically damaging pest on maize, particularly in the maize agri-ecosystems of Jilin Province, northeastern China. Parasitic wasps are important natural enemies of aphids, but limited information exists about their species composition, richness and seasonal dynamics in northeastern China. In this study, the population dynamics of maize aphids and parasitoid wasps were assessed in relation to each other during the summer seasons of two consecutive years, 2018 and 2019. We selected maize fields in the Changchun, Songyuan, Huinan and Gongzhuling areas of Jilin Province. Four species of aphids were recorded from these maize fields: Rhopalosiphum padi (L), Rhopalosiphum maidis (Fitch), Aphis gossypii Glover and Macrosiphum miscanthi (Takahashi). The dominant species in each of the four areas were R. maids (Filch) and R. padi in Changchun, R. padi in Songyuan, A. gossypii and R. padi in Huinan, and A.gossypii and R. padi in Gongzhuling. We delineated a species complex made up of primary parasitoids and hyperparasitoids associated with maize aphids. The primary parasitoids Lysiphlebus testaceipes, Binodoxys communis and Aphelinus albipodus together formed approximately 85.3% of the parasitoid complex. Pachyneuron aphidis, Phaenoglyphis villosa, Syrphophagus taeniatus and Asaphes suspensus made up the hyperparasitoids. Of the primary parasitoids, L. testaceipes was the dominant species (81.31%). Of the hyperparasitoid group, P. villosa was the dominant species (68.42%). Parasitism rates followed the fluctuation of the aphid population. The highest parasitic rate was observed during the peak period of cotton aphids. In this paper, the occurrence dynamics and dominant species of aphids and the dynamics of parasitic natural enemies of aphids in maize fields in Jilin Province are, for the first time, systematically reported. This study provides important information for the establishment and promotion of aphid biological control in maize fields.

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Usefulness of the murine model to study the immune response against Histoplasma capsulatum infection

Cited by 13 publications

References 106 publications

Novel clinical and dual infection by Histoplasma capsulatum genotypes in HIV patients from Northeastern, Brazil

Novel clinical and dual infection by Histoplasma capsulatum genotypes in HIV patients from Northeastern, Brazil

Dimorphism and Dissemination of Histoplasma capsulatum in the Upper Respiratory Tract after Intranasal Infection of Bats and Mice with Mycelial Propagules

Population dynamics and species composition of maize field parasitoids attacking aphids in northeastern China

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